Synovial Gene Signatures Associated with the Development of Rheumatoid Arthritis in At Risk Individuals: a Prospective Study

Abstract

Background: Previous work has shown subtle infiltration of synovial T cells in the absence of overt synovial inflammation in individuals at risk of developing rheumatoid arthritis (RA). Objective: To study the molecular changes in synovium preceding arthritis development in at risk individuals. Materials and methods: We included sixty-seven individuals with arthralgia who were IgM rheumatoid factor (RF) and/or anti-citrullinated protein antibody (ACPA) positive and without any evidence of arthritis. All individuals underwent mini-arthroscopic synovial tissue sampling of a knee joint at baseline and were followed prospectively. An explorative genome-wide transcriptional profiling study was performed on synovial tissue using Agilent arrays (discovery cohort). Survival analysis was used to identify transcripts associated with arthritis after follow up. Expression levels of differentially expressed genes were validated using quantitative real-time PCR (qPCR). Immunohistochemistry was used to study gene candidates at the protein level in situ. Results: In the discovery cohort, 6 of the 13 at risk individuals developed RA after a median follow-up time of 20 months (IQR 2 – 44; pre-RA). The 7 individuals who did not develop RA had a median follow-up time of 85 months (IQR 69 – 86). Using a False Discovery Rate of <5% we found increased expression of 3,151 transcripts correlating with a higher risk of arthritis development, whereas increased expression of 2,437 transcripts correlated with a lower risk. Gene set enrichment analysis revealed that synovial biopsies of pre-RA individuals display higher expression of genes involved in several immune response-related pathways compared with biopsies of individuals who did not develop RA. In contrast, lower expression was observed for genes involved in extracellular matrix receptor interaction, Wnt-mediated signal transduction and lipid metabolism. Two-way hierarchical cluster analysis of 27 genes measured by qPCR classified the synovial biopsies of 61 individuals into two groups, where pre-RA individuals (n=16) showed a preference to cluster together. Synovial tissue from pre-RA individuals were more likely to show podoplanin positive cells and lower lipid staining compared with synovial tissue from individuals who did not develop RA. Conclusion: Molecular changes can be detected in synovial tissues before clinical onset of arthritis. Alterations in the immune response genes and lipid metabolism are associated with development of arthritis.

Translational Research Studies Unraveling the Origins of Psoriatic Arthritis: Moving Beyond Skin and Joints

“…Similar prospective follow-up studies could be applied to other target tissues, but tissues like bone and entheses are in general more challenging to collect in a prospective study. Studying synovial biopsies over time is challenging but possible, even in the absence of arthritis (122)(123)(124)(125)(126)(127)(128). When possible, it is preferred to study serial paired tissue samples of various tissues in the same patients.…”

Bridging Insights From Lymph Node and Synovium Studies in Early Rheumatoid Arthritis

“…This is in line with a prospective study which compared synovial biopsies of RArisk individuals who later developed RA after follow up, with those who did not. Using immunohistochemistry analyses no overt immune cell infiltration was found in the synovium of RA-risk individuals who later developed disease (14,15) while gene expression profiling points toward an activated stromal cell gene signature with increased podoplanin and CXCL12 levels and decreased lipid droplets (16). These studies provide strong evidence for a causal role for FLS in driving disease pathogenesis at an early stage.…”