Synovial mesenchymal stem cells promote healing after meniscal repair in microminipigs

Nakagawa, Yusuke; Muneta, Takeshi; Kondo, Shimpei; Mizuno, Mitsuru; Takakuda, Kazuo; Ichinose, Shizuko; Tabuchi, Takashi; Koga, Hideyuki; Tsuji, Kunikazu; Sekiya, Ichiro

doi:10.1016/j.joca.2015.02.008

Cited by 113 publications

(104 citation statements)

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“…Previously, it has been suggested that the potential of synovial MSCs can be affected by the presence of cartilage injury and the period between the onset of pain and date of surgery [28]. However, in this study, there was no significant difference in growth potential despite varied time frame between onset of pain and date of surgery for both the knee and the hip joints for our patients.…”

Section: Discussioncontrasting

confidence: 66%

Isolation and Characterization of Synovial Mesenchymal Stem Cell Derived from Hip Joints: A Comparative Analysis with a Matched Control Knee Group

Hatakeyama

Uchida

Utsunomiya

et al. 2017

Stem Cells International

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Purpose. To determine the characteristics of MSCs from hip and compare them to MSCs from knee. Methods. Synovial tissues were obtained from both the knee and the hip joints in 8 patients who underwent both hip and knee arthroscopies on the same day. MSCs were isolated from the knee and hip synovial samples. The capacities of MSCs were compared between both groups. Results. The number of cells per unit weight at passage 0 of synovium from the knee was significantly higher than that from the hip (P < 0.05). While it was possible to observe the growth of colonies in all the knee synovial fluid samples, it was impossible to culture cells from any of the hip samples. In adipogenesis experiments, the frequency of Oil Red-O-positive colonies and the gene expression of adipsin were significantly higher in knee than in hip. In osteogenesis experiments, the expression of COL1A1 and ALPP was significantly less in the knee synovium than in the hip synovium. Conclusions. MSCs obtained from hip joint have self-renewal and multilineage differentiation potentials. However, in matched donors, adipogenesis and osteogenesis potentials of MSCs from the knees are superior to those from the hips. Knee synovium may be a better source of MSC for potential use in hip diseases.

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Section: Discussioncontrasting

confidence: 66%

Isolation and Characterization of Synovial Mesenchymal Stem Cell Derived from Hip Joints: A Comparative Analysis with a Matched Control Knee Group

Hatakeyama

Uchida

Utsunomiya

et al. 2017

Stem Cells International

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“…Mesenchymal stem cells derived from synovial membrane (synovial MSCs) are a candidate cell source for regenerative medicine of cartilage [1–3] and meniscus [4–6] due to their great chondrogenic ability [7, 8]. In clinical practice, the transplantation of autologous synovial MSCs for ten patients with a symptomatic single cartilage lesion in the knee joints was shown to be effective in terms of magnetic resonance imaging (MRI) and patient-oriented outcome scoring [9].…”

Section: Introductionmentioning

confidence: 99%

Yields and chondrogenic potential of primary synovial mesenchymal stem cells are comparable between rheumatoid arthritis and osteoarthritis patients

et al. 2017

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BackgroundMesenchymal stem cells derived from the synovial membrane (synovial MSCs) are a candidate cell source for regenerative medicine of cartilage and menisci due to their high chondrogenic ability. Regenerative medicine can be expected for RA patients with the inflammation well-controlled as well as OA patients and transplantation of synovial MSCs would also be a possible therapeutic treatment. Some properties of synovial MSCs vary dependent on the diseases patients have, and whether or not the pathological condition of RA affects the chondrogenesis of synovial MSCs remains controversial. The purpose of this study was to compare the properties of primary synovial MSCs between RA and OA patients.MethodsHuman synovial tissue was harvested during total knee arthroplasty from the knee joints of eight patients with RA and OA respectively. Synovial nucleated cells were cultured for 14 days. Total cell yields, surface markers, and differentiation potentials were analyzed for primary synovial MSCs.ResultsNucleated cell number per 1 mg synovium was 8.4 ± 3.9 thousand in RA and 8.0 ± 0.9 thousand in OA. Total cell number after 14-day culture/1 mg synovium was 0.7 ± 0.4 million in RA and 0.5 ± 0.3 million in OA, showing no significant difference between in RA and OA. Cells after 14-day culture were mostly positive for CD44, CD73, CD90, CD105, negative for CD45 both in RA and OA. There was no significant difference for the cartilage pellet weight and sGAG content per pellet between in RA and OA. Both oil red O-positive colony rate and alizarin red-positive colony rate were similar in RA and OA.ConclusionsYields, surface markers and chondrogenic potential of primary synovial MSCs in RA were comparable to those in OA. Synovium derived from RA patients can be the cell source of MSCs for cartilage and meniscus regeneration.Electronic supplementary materialThe online version of this article (doi:10.1186/s13287-017-0572-8) contains supplementary material, which is available to authorized users.

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“…While MSCs have been employed in many of the cellbased meniscal repair studies, 37,38 meniscus-derived cells could be a good cell source because these cells showed strong proliferative and fibrocartilaginous differentiation capacities, as reported in previous studies. 29,30 Also, regarding the method of cell isolation, we isolated MFCs from small pieces of meniscal tissue without collagenase, using a previously published method.…”

Section: Discussionmentioning

confidence: 80%

In Vitro Repair of Meniscal Radial Tear Using Aligned Electrospun Nanofibrous Scaffold

Shimomura

Bean

Lin

et al. 2015

Tissue Engineering Part A

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Synovial mesenchymal stem cells promote healing after meniscal repair in microminipigs

Abstract: Transplantation of synovial MSCs promoted healing after meniscal repair with induction of synovium into the longitudinal tear in the avascular zone of meniscus in pigs.

Cited by 113 publications

References 38 publications

Isolation and Characterization of Synovial Mesenchymal Stem Cell Derived from Hip Joints: A Comparative Analysis with a Matched Control Knee Group

Isolation and Characterization of Synovial Mesenchymal Stem Cell Derived from Hip Joints: A Comparative Analysis with a Matched Control Knee Group

Yields and chondrogenic potential of primary synovial mesenchymal stem cells are comparable between rheumatoid arthritis and osteoarthritis patients

In Vitro Repair of Meniscal Radial Tear Using Aligned Electrospun Nanofibrous Scaffold

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