Syntaxins 13 and 7 Function at Distinct Steps During Phagocytosis

Collins, Richard F.; Schreiber, Alan D.; Grinstein, Sergio; Trimble, William S.

doi:10.4049/jimmunol.169.6.3250

Cited by 84 publications

(67 citation statements)

References 33 publications

(27 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The identification of trans-complexes between V0 domains in adjacent membranes showing the same dependence on inhibitors and activators as the fusion process itself led to the proposal that, after SNARE mediated docking, these transcomplexes promote the mixing of the lipid bilayers by virtue of the highly hydrophobic proteolipid subunits (50). Interestingly, our results confirmed that the SNARE protein syntaxin 7 accumulates in late phagosomes (51), supporting the notion that this enrichment occurs specifically in detergent-insoluble parts of the membrane. The role of the lipid environment as regulator of V-ATPase activity was addressed by Lafoucade and colleagues who concluded that the DRMs association increased the pump activity by promoting V1-V0 coupling (52).…”

Section: Discussionsupporting

confidence: 77%

Proteomic Characterization of Phagosomal Membrane Microdomains During Phagolysosome Biogenesis and Evolution

Goyette

Boulais

Carruthers

et al. 2012

Molecular & Cellular Proteomics

View full text Add to dashboard Cite

After their formation at the cell surface, phagosomes become fully functional through a complex maturation process involving sequential interactions with various intracellular organelles. In the last decade, series of data indicated that some of the phagosome functional properties occur in specialized membrane microdomains. The molecules associated with membrane microdomains, as well as the organization of these structures during phagolysosome biogenesis are largely unknown. In this study, we combined proteomics and bioinformatics analyses to characterize the dynamic association of proteins to maturing phagosomes. Our data indicate that groups of proteins shuffle from detergent-soluble to detergent-resistant membrane microdomains during maturation, supporting a model in which the modulation of the phagosome functional properties involves an important reorganization of the phagosome proteome by the coordinated spatial segregation of proteins.

show abstract

Section: Discussionsupporting

confidence: 77%

Proteomic Characterization of Phagosomal Membrane Microdomains During Phagolysosome Biogenesis and Evolution

Goyette

Boulais

Carruthers

et al. 2012

Molecular & Cellular Proteomics

View full text Add to dashboard Cite

show abstract

“…VAMP-8 has been reported to form complex with Syn-7, Syn-8, and Vti1b, which mediates homotypic fusion of early and late endosomes (15,17,18). As reported previously, Syn-7 was recruited to phagosomes in macrophages (31). Interestingly, this same set of Syn-7-containing Q-SNAREs (Syn-7-Syn-8-Vti1b) also partners with VAMP-7 for mediating heterotypic fusion of the late endosomes and lysosomes (18).…”

Section: Discussionmentioning

confidence: 59%

Vesicle-Associated Membrane Protein-8/Endobrevin Negatively Regulates Phagocytosis of Bacteria in Dendritic Cells

Cai

Wang

et al. 2008

The Journal of Immunology

View full text Add to dashboard Cite

Phagocytosis is a specialized mechanism used by mammalian cells, particularly the cells of the immune system, such as dendritic cells (DC) and macrophages, to protect the host against infection. The process involves a complex cascade of pathways, from the ligation of surface receptors of phagocytes with components of the microorganism’s surface, formation of phagosomes and subsequently phagolysosomes, to the eventual presentation of foreign Ags. Vesicle-associated membrane protein (VAMP)-8/endobrevin has been shown previously to function in the endocytic pathways. Our results showed that VAMP-8 colocalized with lysosome-associated membrane protein-2, and a significant amount of VAMP-8 was recruited to the phagosomes during bacterial ingestion. However, overexpression of VAMP-8 significantly inhibited phagocytosis in DC. We also found that the phagocytic activity of VAMP-8−/− DC was significantly higher than wild-type VAMP-8+/+ DC, thus further confirming that VAMP-8 negatively regulates phagocytosis in immature DC.

show abstract

“…Candidates are, for example, STX7 which has been found to reside in late endosomes and to participate in the process of phagocytosis [20]; alternatively or in addition STX18, which has been implicated in endoplasmic reticulum-mediated phagocytosis in J774 macrophages [21]. Furthermore, STX4 and STX6, which are regulated in LPS activated RAW264.7 macrophages, have been described to regulate the exocytosis of TNF [22,23] in these cells.…”

Section: Discussionmentioning

confidence: 99%

Syntaxin 11 is required for NK and CD8⁺ T‐cell cytotoxicity and neutrophil degranulation

et al. 2012

View full text Add to dashboard Cite

Syntaxin 11 (STX11) controls vesicular trafficking and is a key player in exocytosis. SinceStx11 mutations are causally associated with a familial hemophagocytic lymphohistiocytosis, we wanted to clarify whether STX11 is functionally important for key immune cell populations. This was studied in primary cells obtained from newly generated Stx11 −/− mice. Our data revealed that STX11 is not only widely expressed in different immune cells, but also induced upon LPS or IFN-γ treatment. However, Stx11 deficiency does not affect macrophage phagocytic function and cytokine secretion, mast cell activation, or antigen presentation by DCs. Instead, STX11 selectively controls lymphocyte cytotoxicity in NK and activated CD8 + T cells and degranulation in neutrophils. Stx11 −/− NK cells and CTLs show impaired degranulation, despite a comparable activation, maturation and expression of the complex-forming partners MUNC18-2 and VTI1B. In addition, Stx11 −/− CTLs and NK cells produce abnormal levels of IFN-γ. Since functional reconstitution rescues the defective phenotype of Stx11 −/− CTLs, we suggest a direct, specific and key role of STX11 in controlling lymphocyte cytotoxicity, cytokine production and secretion. Finally, we show that these mice are a very useful tool for dissecting the role of STX11 in vesicular trafficking and secretion. Keywords: CTL r Cytokines r Exocytosis r N-ethylmaleimide-sensitive factor attachment protein receptorSee accompanying Commentary by Lopez and Voskoboinik.Additional supporting information may be found in the online version of this article at the publisher's web-site IntroductionSyntaxin 11 (STX11) belongs to the family of N-ethylmaleimidesensitive factor attachment protein receptor (SNARE) proteins Correspondence: Prof. Silvia Bulfone-Paus e-mail: sbulfone@fz-borstel.de and is involved in intracellular membrane trafficking events by interacting with other family members or by associating with membranes by palmitoylation of cysteine residues [1][2][3]. STX11 is expressed in a wide variety of cells including human monocytes/macrophages, neutrophils, B cells, NK cells and CD8 + T cells [2][3][4][5][6][7][8][9]. STX11 is enriched in immune tissues such as thymus, spleen C 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim www.eji-journal.eu Eur. J. Immunol. 2013. 43: 194-208 Immunomodulation 195 Values represent the mean ± SEM percentage of cell subsets in the spleen (n = 8) and lymph nodes (WT n = 7; Stx11 −/− n = 6) and are the summary of three experiments performed.and lymph nodes, but lower levels of protein are detectable also in heart, kidney and liver [2]. STX11 localizes mainly in the vesicular tubular clusters, an organelle complex between the endoplasmic reticulum and the Golgi, and displays, in addition, a spotted staining pattern throughout the cell periphery [2,9]. While STX11 has been recognized to act as a negative regulator of both phagocytosis and intracellular trafficking between endosomes, lysosomes and the outer membrane in macrophages [6,9], in human NK cells and CTLs, ST...

show abstract

Syntaxins 13 and 7 Function at Distinct Steps During Phagocytosis

Cited by 84 publications

References 33 publications

Proteomic Characterization of Phagosomal Membrane Microdomains During Phagolysosome Biogenesis and Evolution

Proteomic Characterization of Phagosomal Membrane Microdomains During Phagolysosome Biogenesis and Evolution

Vesicle-Associated Membrane Protein-8/Endobrevin Negatively Regulates Phagocytosis of Bacteria in Dendritic Cells

Syntaxin 11 is required for NK and CD8⁺ T‐cell cytotoxicity and neutrophil degranulation

Contact Info

Product

Resources

About

Syntaxins 13 and 7 Function at Distinct Steps During Phagocytosis

Cited by 84 publications

References 33 publications

Proteomic Characterization of Phagosomal Membrane Microdomains During Phagolysosome Biogenesis and Evolution

Proteomic Characterization of Phagosomal Membrane Microdomains During Phagolysosome Biogenesis and Evolution

Vesicle-Associated Membrane Protein-8/Endobrevin Negatively Regulates Phagocytosis of Bacteria in Dendritic Cells

Syntaxin 11 is required for NK and CD8+ T‐cell cytotoxicity and neutrophil degranulation

Contact Info

Product

Resources

About

Syntaxin 11 is required for NK and CD8⁺ T‐cell cytotoxicity and neutrophil degranulation