Synthèse d'acide désoxyribonucléique dans le corps parabasal de Trypanosoma mega

Steinert, G.; Firket, H; Steinert, Maurice

doi:10.1016/0014-4827(58)90117-4

Cited by 65 publications

(16 citation statements)

References 4 publications

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“…In longitudinal sections, this inclusion showed a lamellar, almost spiral-like structure that was not separated by a membrane from the surrounding electron transparent space of the vacuole. At the same time, Steinert et al (1958) demonstrated that the kinetoplast incorporates [ 3 H] thymidine into the fibrillar structure, thus confirming the presence of DNA. Further studies using electron microscope autoradiography, performed by Burton and Dusanic (1968) as well as Anderson and Hill (1969), showed the dynamics of 3 H thymidine incorporation during kinetoplast replication.…”

Section: The Kinetoplastsupporting

confidence: 50%

Electron microscopy of trypanosomes: a historical view

Souza

2008

Mem. Inst. Oswaldo Cruz

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Since the discovery of the electron microscope and the development of the initial techniques for the processing of biological samples for electron microscopy, the protozoan Trypanosoma cruzi has been the subject of intense investigation. This review analyzes the results obtained by observation of whole trypanosomes as well as thin sections and replicas using several microscopic approaches. Micrographs detailing the appearance of T. cruzi using several methods illustrate the evolution of electron microscopic techniques as well as its contribution to understanding the structural organization of the protozoan.

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Section: The Kinetoplastsupporting

confidence: 50%

Electron microscopy of trypanosomes: a historical view

Souza

2008

Mem. Inst. Oswaldo Cruz

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“…The T. cruzi kinetoplast lamellar, almost spiral-like structure is retained in a membrane lining a vacuole (378). The remarkable molecular configuration of the kDNA network (279,326,360) continues directly into a typical mitochondrion canal system, as seen for any tissue cell (309).…”

Section: Kinetoplast Dnamentioning

confidence: 99%

Pathogenesis of Chagas' Disease: Parasite Persistence and Autoimmunity

et al. 2011

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SUMMARY Acute Trypanosoma cruzi infections can be asymptomatic, but chronically infected individuals can die of Chagas' disease. The transfer of the parasite mitochondrial kinetoplast DNA (kDNA) minicircle to the genome of chagasic patients can explain the pathogenesis of the disease; in cases of Chagas' disease with evident cardiomyopathy, the kDNA minicircles integrate mainly into retrotransposons at several chromosomes, but the minicircles are also detected in coding regions of genes that regulate cell growth, differentiation, and immune responses. An accurate evaluation of the role played by the genotype alterations in the autoimmune rejection of self-tissues in Chagas' disease is achieved with the cross-kingdom chicken model system, which is refractory to T. cruzi infections. The inoculation of T. cruzi into embryonated eggs prior to incubation generates parasite-free chicks, which retain the kDNA minicircle sequence mainly in the macrochromosome coding genes. Crossbreeding transfers the kDNA mutations to the chicken progeny. The kDNA-mutated chickens develop severe cardiomyopathy in adult life and die of heart failure. The phenotyping of the lesions revealed that cytotoxic CD45, CD8 + γδ, and CD8α + T lymphocytes carry out the rejection of the chicken heart. These results suggest that the inflammatory cardiomyopathy of Chagas' disease is a genetically driven autoimmune disease.

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“…Examples are the kinetoplasts of Trypanosoma (22), chloroplasts of Chlamydomonas (20), and 0.3 to 0.5 # particles in Amoeba (18).…”

Section: N O T E S M I T O C H O N D R I a L I N C O R P O R A T I O mentioning

confidence: 99%