Synthesen verschiedenartig <sup>3</sup>H‐markierter putrescine

Fischer, Hartmut; Seiler, Nikolaus; Werner, G.

doi:10.1002/jlcr.2590070213

Cited by 14 publications

(3 citation statements)

References 5 publications

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“…radioactivity 23Ci/mol) and y-amino[1-14C]butyric acid (sp. radioactivity 3.6Ci/mol) were purchased from New England Nuclear Corp., Boston, Mass., U.S.A.; [2,3-3H]putrescine dihydrochloride was synthesized as described by Fischer et al (1971). Monoacetyl[2, hydrochloride.…”

Section: Materials and Methods Materialsmentioning

confidence: 99%

Putrescine catabolism in mammalian brain

Seiler

Al‐Therib

1974

Biochemical Journal

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197

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In contrast with putrescine (1,4-diaminobutane), which is a substrate of diamine oxidase, monoacetylputrescine is oxidatively deaminated both in vitro and in vivo by monoamine oxidase. The product of this reaction is N-acetyl-gamma-aminobutyrate. The existence of a degradative pathway in mammalian brain for putrescine is shown, which comprises acetylation of putrescine, oxidative deamination of monoacetylputrescine to N-acetyl-gamma-aminobutyrate, transformation of N-acetyl-gamma-aminobutyrate to gamma-aminobutyrate and degradation of gamma-aminobutyrate to CO(2) via the tricarboxylic acid cycle.

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Section: Materials and Methods Materialsmentioning

confidence: 99%

Putrescine catabolism in mammalian brain

Seiler

Al‐Therib

1974

Biochemical Journal

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197

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“…With the use of a system described previously (10), we demonstrated axonal transport of putrescine and compared its characteristics to axonal transport of protein. We synthesized [2,3-3H]putrescine dihydrochloride, specific activity 744 mc/ mmole, by catalytic tritiation of 1,4diaminobutine (11). About 0.005 ,ul (5 nc) of a solution of 5 mg of the labeled putrescine in 1 ml of Sorensen phosphate buffer, pH 7.4, was injected into one eye of 7-day-old Brachydanio rerio (zebrafish) embryos through a glass micropipette with a micromanipulator.…”

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confidence: 99%

Axonal Transport of Tritium-Labeled Putrescine in the Embryonic Visual System of Zebrafish

Fischer

Schmatolla

1972

Science

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The transport of [(3)H]putrescine is demonstrated by autoradiography in the retino-tectal tract of Brachydanio rerio embryos. Transport of [(3)H]putrescine appears to be more rapid than that of tritium-labeled protein and is not inhibited by a colchicine effect on axonal neurotubules as is protein transport. The radioactivity transported to the brain is found, on electrophoresis, in the putrescine fraction.

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“…Sato et al (10) have found the internal ionic concentrations of Aplysia neurons to be similar to those of other cells, and Brown and his co-workers (11,12) have shown with ion-specific electrodes that the internal activities of K+ and Clare not very different from those that would be expected from the known internal concentrations. Therefore, ions are present and are not bound.…”

Section: Frankfurtim Germanymentioning

confidence: 93%