Syntheses and structures of bismuth(III) complexes with nitrilotriacetic acid, ethylenediaminetetraacetic acid, and diethylenetriaminepentaacetic acid

Periodic Iron Nanomineralization in Human Serum Transferrin Fibrils

“…Fe(NTA) and Bi(NTA) were synthesized as reported. [26][27][28][29] Metal reloading: To reload with iron or bismuth, apo-hTf (1 mm) was incubated at 37 8C with Fe(NTA) or Bi(NTA) (3 mol equiv) in 10 mm HEPES and 5 mm NaHCO 3 solution at pH 7.4 for two days. These solutions were then extensively dialyzed (using Sigma-Aldrich (Z368 474-10EA) disposable dialyzers, MWCO 8000 Da, size 1 mL, diameter 5 mm, regenerated cellulose membrane) against 5 mm sodium bicarbonate solution and used for transmission electron microscopy.…”

Indirect detection of protein-Metal binding: Interaction of serum transferrin with In3+ and Bi3+

“…In 3ϩ · NTA was prepared by mixing the appropriate quantity of the metal (10,000 g/ml In 3ϩ in 5% HNO 3 , plasma standard solution from Alfa Aesar, Ward Hill, MA) with Na 2 HNTA (Sigma Chemical Co.). Bi 3ϩ · NTA was prepared from bismuth subcarbonate (BiO) 2 CO 3 (Sigma Chemical Co.) using a procedure described elsewhere [14]. Metal binding to hTf in solution was monitored by measuring difference UV absorption spectra with a UV-160PC (Shimadzu, Tokyo, Japan) UV-Vis spectrophotometer.…”

Competitive Binding of Bismuth to Transferrin and Albumin in Aqueous Solution and in Blood Plasma

“…NaHCO 3 , KCl, 5,5Ј-dithiobis(2-nitrobenzoic acid), and other chemicals were purchased either from Aldrich or Sigma with the highest quality and used as received. Crystalline [Bi(NTA)] was synthesized according to a literature procedure (30) M) was added to the samples to give a final concentration of 10 mM, and pH* values were adjusted using NaOD or DCl. For the intact blood plasma experiments, blood from a male healthy volunteer was collected by venipuncture into lithiumheparinized vacutainers, and the plasma was separated by centrifugation at 6000 rmp for 20 min at 277 K, and stored frozen until used for NMR measurements.…”