Synthesis, accumulation and hydrolysis of trehalose during growth of peanut rhizobia in hyperosmotic media

Dardanelli, Marta Susana; González, Pablo J.; Bueno, Miguel Ángel Beltrán; Ghittoni, Nora E.

doi:10.1002/1521-4028(200007)40:3<149::aid-jobm149>3.0.co;2-y

Cited by 11 publications

(1 citation statement)

References 31 publications

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“…Free-living rhizobia were shown to synthesize trehalose in response to phenanthrene, hyperosmolarity, high temperature, salt stress, very low oxygen levels, and desiccation (Hoelzle and Streeter, 1990;Ghittoni and Bueno, 1996;Dardanelli et al, 1997Dardanelli et al, , 2000Cytryn et al, 2007;Essendoubi et al, 2007;McIntyre et al, 2007;Keum et al, 2008). Furthermore, the resistance of Rhizobium etli to salt stress was improved in a strain overexpressing a trehalose 6-phosphate synthase gene and decreased in a strain mutated in the same gene (Suarez et al, 2008).…”

Section: Trehalose: a Disaccharide Required For Root Hair Colonizationmentioning

confidence: 99%

Soybean Metabolites Regulated in Root Hairs in Response to the Symbiotic BacteriumBradyrhizobium japonicum

et al. 2010

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Nodulation of soybean (Glycine max) root hairs by the nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum is a complex process coordinated by the mutual exchange of diffusible signal molecules. A metabolomic study was performed to identify small molecules produced in roots and root hairs during the rhizobial infection process. Metabolites extracted from roots and root hairs mock inoculated or inoculated with B. japonicum were analyzed by gas chromatography-mass spectrometry and ultraperformance liquid chromatography-quadrupole time of flight-mass spectrometry. These combined approaches identified 2,610 metabolites in root hairs. Of these, 166 were significantly regulated in response to B. japonicum inoculation, including various (iso)flavonoids, amino acids, fatty acids, carboxylic acids, and various carbohydrates. Trehalose was among the most strongly induced metabolites produced following inoculation. Subsequent metabolomic analyses of root hairs inoculated with a B. japonicum mutant defective in the trehalose synthase, trehalose 6-phosphate synthase, and maltooligosyltrehalose synthase genes showed that the trehalose detected in the inoculated root hairs was primarily of bacterial origin. Since trehalose is generally considered an osmoprotectant, these data suggest that B. japonicum likely experiences osmotic stress during the infection process, either on the root hair surface or within the infection thread.

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Section: Trehalose: a Disaccharide Required For Root Hair Colonizationmentioning

confidence: 99%

Soybean Metabolites Regulated in Root Hairs in Response to the Symbiotic BacteriumBradyrhizobium japonicum

et al. 2010

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Physiology Of Root-Nodule Bacteria

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Hynes

Johnston

et al.

Nitrogen-Fixing Leguminous Symbioses

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Stress Physiology in Azospirillum and Other PGPRs

Gallarato

Paulucci

Chiappero

et al. 2015

Handbook for Azospirillum

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Rhizobacteria are constantly faced with environmental stimuli stresses and should be responding to a wide range of factors through signal transduction pathways that convert extracellular information into intracellular forms. The cytoplasmic membrane of bacteria is permeable to water but forms an effective barrier for most solute present in the cytoplasmic. To survive osmotic stresses, rhizobacteria need to adapt by accumulating specifi c solutes under hyperosmotic conditions and releasing them under hyposmotic conditions. These solutes are referred to compatible solutes and glutamate, K + , trehalose, betaine, among others solutes, are used to response. But new studies to proteins, phospholipids, and polysaccharides and their pattern in response to stress are necessary.

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Synthesis, accumulation and hydrolysis of trehalose during growth of peanut rhizobia in hyperosmotic media

Cited by 11 publications

References 31 publications

Soybean Metabolites Regulated in Root Hairs in Response to the Symbiotic BacteriumBradyrhizobium japonicum

Soybean Metabolites Regulated in Root Hairs in Response to the Symbiotic BacteriumBradyrhizobium japonicum

Physiology Of Root-Nodule Bacteria

Stress Physiology in Azospirillum and Other PGPRs

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