Synthesis and adsorption of a poly(n‐acetylethyleneimine)‐polyethyleneoxide‐poly (n‐acetylethyleneimine) triblock‐copolymer at a silica/solution interface. influence of its preadsorption on platelet adhesion and fibrinogen adsorption

Abstract

The synthesis of a triblock copolymer poly-(N-acetylethyleneimine)-polyethylenoxide-poly(N-acet ylethyleneimine) includes two successive steps: the first is the functionalization of a poly(ethyleneglycol) precursor by creating sulfonic esters at its chain ends, the second uses these esters to initiate the cationic polymerization of 2-methyl-2-oxazoline. Homopolymers appear in the raw product; hence successive selective extractions of the copolymer with benzene and dioxane are necessary. The final yield in pure copolymer was 11%. The copolymer was characterized by UV and 1H-NMR spectrometry and light scattering. Adsorption isotherms were determined on silica, for varying pH and salt concentration. Optimum conditions for coating silica with the polymer were determined. The efficiency of this precoating to reduce the adsorption of fibrinogen was very high (99.2% reduction with respect to bare silica). Steric exclusion chromatography of a variety of proteins gave a satisfactory calibration curve. Platelet accumulation on copolymer precoated glass was reduced to 10-20% of its value on bare glass, a result superior to that obtained by albumin passivation of the same glass surface.

Polymerization of cyclic imino ethers: From its discovery to the present state of the art

“…A block copolymer of PMeOZO and PEO has been employed for coating the support silica to improve blood compatibility 89. The efficiency of this coating to reduce the adsorption of fibrinogen is very high (99% reduction with respect to bare silica).…”

Deposition of 2‐oxazoline‐based plasma polymer coatings using atmospheric pressure helium plasma jet

“…However, the lengthy and complex synthetic routes, as well as the lack of applications for this class of polymers at the time, inhibited their appeal for commercial mass production. In recent years, the interest in POx has grown substantially, largely due to the discovery that their excellent biocompatibility and “stealth” behaviour are comparable to the gold standard polyethylene glycol . For many applications, especially in the biomedical field, POx are applied onto biomaterials surface as thin film coatings from aqueous solutions .…”

Surface Functionalization and Patterning Techniques to Design Interfaces for Biomedical and Biosensor Applications

“…However, several parameters (chain density, chain length, chain conformation) of the coating need to be involved in the evaluation of the properties of the films 23. Different techniques have been successfully used for producing PEO layers: physical adsorption,24 radiation and chemical cross linking,25 self‐assembled monolayers,26 spin coating stabilized by ion beam treatment,27 chemisorption of PEO thiol on gold surfaces 28. However, these techniques present some drawbacks since they can only be used on a few types of substrate and involve several chemical steps with solvents.…”