Synthesis and Application of Amine Functionalized Iron Oxide Nanoparticles on Menaquinone-7 Fermentation: A Step towards Process Intensification

Abstract

Industrial production of menaquione-7 by Bacillus subtilis natto is associated with major drawbacks. To address the current challenges in menaquione-7 fermentation, studying the effect of magnetic nanoparticles on the bacterial cells can open up a new domain for intensified menqainone-7 process. This article introduces the new concept of production and application of l-lysine coated iron oxide nanoparticles (l-Lys@IONs) as a novel tool for menaquinone-7 biosynthesis. l-Lys@IONs with the average size of 7 nm were successfully fabricated and were examined in a fermentation process of l-Lys@IONs decorated Bacillus subtilis natto. Based on the results, higher menaquinone-7 specific yield was observed for l-Lys@IONs decorated bacterial cells as compared to untreated bacteria. In addition, more than 92% removal efficacy was achieved by using integrated magnetic separation process. The present study demonstrates that l-Lys@IONs can be successfully applied during a fermentation of menaquinone-7 without any negative consequences on the culture conditions. This study provides a novel biotechnological application for IONs and their future role in bioprocess intensification.

Green Synthesis of Silver Nanoparticles using Mediterranean Cypress (<i>Cupressus sempervirens</i>) Leaf Extract

“…5b) (Ebrahiminezhad et al, 2016f;2015b). The main diffraction peaks appeared at 38 and 44° of 2è degrees (Ebrahiminezhad et al, 2016b;2016c;2016e).…”

Cyclic voltammetry, photocatalytic and antimicrobial comparative studies of fabrication Fe3O4 and Fe3O4/PAN nanofibers

“…Functionalization of different polymers with different nanoparticles to form their corresponding nanocomposites has been reported. Iron oxide (Fe 3 O 4 ) nanoparticles and its nanocomposites have shown great applications in magnetic recording [19], toners [20], wastewater treatment [21][22][23][24], medicine [25], bioprocesses [26,27], magnetic resonance imaging [28][29][30], magnetic data storage devices [19], and xerography ink [20]. Furthermore, polymeric nanofibers and their nanocomposites are formed by electrospinning process [31].…”

Characterization of biogenic Fe (III)‐binding exopolysaccharide nanoparticles produced by Ralstonia sp. SK03

“…9,11,14 In this regard, new strategies include using natural resources, such as polysaccharides, biodegradable polymers, amino acids, and plant extracts or direct production by microorganisms, plants, and human cells. [15][16][17] Among microorganisms, bacteria have a significant ability to reduce metal ions and synthesize nanoparticles with desirable properties such as high stability, controlled size and shape, and monodispersity in ambient temperatures and pressures and at a neutral pH. 9,14 Examples include formation of Fe (III)-binding exopolysaccharide (Fe-EPS) nanoparticles by K. oxytoca, 18 synthesis of ZnS nanoparticles by sulfate reducing bacteria, 19 production of silver nanoparticles by culture supplement of iron reducing bacteria (E. coli and K. pneumoniae), 20 and production of magnetosome (magnetite nanoparticles surrounded by a lipid bilayer) using magnetotactic bacteria.…”