Synthesis and application of caged peptides and proteins

Shigeri, Yasushi; Tatsu, Yoshiro; Yumoto, Noboru

doi:10.1016/s0163-7258(01)00148-6

Cited by 75 publications

(43 citation statements)

References 53 publications

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“…There is a concern about heating of the specimen caused by the flashlight, but it can be alleviated or mitigated by the use of thick, aluminum-coated grids [31]. The flashphotolysis method is mainly limited by the availability of photoactive substrates, including small molecules, peptides and proteins (reviewed in [32]). …”

Section: Time-resolved Cryo-emmentioning

confidence: 99%

Two promising future developments of cryo-EM: capturing short-lived states and mapping a continuum of states of a macromolecule

Chen

Frank²

2015

Microscopy (Tokyo)

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The capabilities and application range of cryogenic electron microscopy (cryo-EM) method have expanded vastly in the last two years, thanks to the advances provided by direct detection devices and computational classification tools. We take this review as an opportunity to sketch out promising developments of cryo-EM in two important directions: (i) imaging of short-lived states (10-1000 ms) of biological molecules by using timeresolved cryo-EM, particularly the mixing-spraying method and (ii) recovering an entire continuum of coexisting states from the same sample by employing a computational technique called manifold embedding. It is tempting to think of combining these two methods, to elucidate the way the states of a molecular machine such as the ribosome branch and unfold. This idea awaits further developments of both methods, particularly by increasing the data yield of the time-resolved cryo-EM method and by developing the manifold embedding technique into a user-friendly workbench.

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Section: Time-resolved Cryo-emmentioning

confidence: 99%

Two promising future developments of cryo-EM: capturing short-lived states and mapping a continuum of states of a macromolecule

Chen

Frank²

2015

Microscopy (Tokyo)

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“…However, we believe that the methods we applied here should also be applicable to measure reaction kinetics of a variety of biochemical processes. Ever since the first reported synthesis of a caged compound (ATP, [64]) the use of caged substances has been continuously developed, leading to the successful experimental use of more than 60 caged molecules of biological importance [65, 66], many of which are commercially available (e.g., Calbiochem, Invitrogen, Tocris, Probior, Enzo Life Sciences). Initially, only ions and small molecules could be caged such as nucleotides (and analogues), gasses (e.g., O 2 , CO and NO), simple neurotransmitters (glutamate, GABA and 5-HT), and simple intracellular messengers (e.g., DAG and IP 3 ), as it is easier to shield their active domain.…”

Section: The Future Of Flash Photolysis In Biomolecular Kineticsmentioning

confidence: 99%

Measuring the kinetics of calcium binding proteins with flash photolysis

Faas

Módy

2012

Biochimica et Biophysica Acta (BBA) - General Subjects

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Background Calcium-binding proteins (CBPs) are instrumental in the control of Ca2+ signaling. They are the fastest players within the Ca2+ toolkit responding within microseconds to [Ca2+] changes. The CBPs compete for Ca2+ which plays a direct role in modulating Ca2+ transients and the resulting biochemical message. The kinetic properties of the CBPs have to be known to have a good understanding of Ca2+ signaling. Scope of review Most techniques used to measure binding kinetics are too slow to accurately determine the fast kinetics of most CBP. Furthermore, many CBPs bind Ca2+ in a cooperative way, which should be incorporated in the kinetic modeling. Here we will review a new ultra-fast in vitro technique for measuring Ca2+ binding properties of CBPs following flash photolysis of caged Ca2+. Compartmental modeling is used to resolve the kinetics of fast cooperative Ca2+ binding to CBPs. Major conclusions Currently this technique has only been used to quantify the kinetics of three CBPs (calbindin, calretinin and calmodulin), but has already provided remarkable insights into the specific role that these kinetics in Ca2+ signaling. General significance The potential to gain novel insights into Ca2+ signaling by quantifying kinetics of other CBPs using this technique is very promising.

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“…; protection/deprotection in organic synthesis [1][2][3][4] and the uncaging of chemically masked or caged biorelevant molecules). [5][6][7][8][9][10][11][12][13][14] For example, the photolysis of sulfonic esters, [15][16][17][18] sulfonamides, [19][20][21][22] and sulfones 23,24) has been applied to photoresist materials, 16,20) protecting groups, 6,17,21,22) and other chemical reactions. 15,24,25) We previously reported that substituted 8-quinolinyl sulfonates such as 1a, e and f undergo photochemical S-O bond cleavage reactions to afford the corresponding 8-quinolinols (2a, e, f) and sulfonate (3) by photoirradiation of aqueous solutions at ca.…”

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confidence: 99%

Photochemical Cleavage Reaction of 8-Quinolinyl Sulfonates That Are Halogenated and Nitrated at the 7-Position

Ariyasu

Hanaya

Tsunoda

et al. 2011

CHEMICAL & PHARMACEUTICAL BULLETIN

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Photochemical bond-cleavage reactions are potentially useful in chemistry, bioorganic chemistry and medicinal chemistry. We previously reported on a photochemical cleavage reaction of 8-quinolinyl sulfonate (8-QS) derivatives in aqueous solution at neutral pH, which we proposed to proceed via an excited triplet state. In this report, we report on the synthesis of some new photocleavable 8-QS derivatives, in which halogen atoms or a nitro group was introduced at the 7-position, in an attempt to improve photoreactive properties and to produce a red-shift in the irradiation wavelength. The introduction of bromine and iodine resulted in an acceleration in the photoreaction by about 1.5 times, possibly due to a heavy atom effect. It was also found that 7-nitro-8-QS absorbs at >360 nm, and, as a result, the S-O bond of this compound can be cleaved by photoirradiation with a fluorescent lamp in aqueous solution and on silicon surface.

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Synthesis and application of caged peptides and proteins

Cited by 75 publications

References 53 publications

Two promising future developments of cryo-EM: capturing short-lived states and mapping a continuum of states of a macromolecule

Two promising future developments of cryo-EM: capturing short-lived states and mapping a continuum of states of a macromolecule

Measuring the kinetics of calcium binding proteins with flash photolysis

Photochemical Cleavage Reaction of 8-Quinolinyl Sulfonates That Are Halogenated and Nitrated at the 7-Position

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