Synthesis and application of new microcarriers for animal cell culture. Part I: Design of polystyrene based microcarriers

Zühlke, Anita; Röder, Bettina; Widdecke, Hartmut; Klein, Joachim

doi:10.1163/156856294x00653

Cited by 13 publications

(5 citation statements)

References 24 publications

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“…Surfaces bearing polar functional groups offer enhanced biological performance by improving cell attachment to the surface 28,33 as well as cell growth. 34 Regarding the reduction of PHB powder, which leads to obtaining oligodiols, the functionalization of the surface of polymer devices with much smaller surface area could generate hydroxyl groups on the outermost face without causing any visible destruction in the modied material. In fact, using lithium borohydride as the reducing agent generates free hydroxyl groups on the outermost face of PHB devices.…”

Section: Electrospray Ionization Mass Spectrometry (Esi-ms)mentioning

confidence: 99%

The heterogeneous selective reduction of PHB as a useful method for preparation of oligodiols and surface modification

et al. 2017

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Section: Electrospray Ionization Mass Spectrometry (Esi-ms)mentioning

confidence: 99%

The heterogeneous selective reduction of PHB as a useful method for preparation of oligodiols and surface modification

et al. 2017

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“…In general, the geometry, size, and the surface property of microcarriers have been shown to significantly affect stem cell expansion and differentiation . Among the commercially available microcarriers, one major category is the solid, spherical or disc‐shaped particles made of dextran, cellulose or polystyrene, which provide a large external surface for cell attachment . The surface of microcarriers can be modified to enhance cell attachment or induce differentiation of stem cells .…”

Section: Bioreactor Systems For Adherent Culture Of Stem Cellsmentioning

confidence: 99%

Stem cell engineering in bioreactors for large‐scale bioprocessing

Liu

Zang

Yang

et al. 2013

Engineering in Life Sciences

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Stem cells are promising cell sources for many biomedical applications including cell therapy, regenerative medicine, and drug discovery. However, the commonly used static tissue culture vessels can only generate a low number of cells. To provide an adequate number of stem cells for clinical applications, a scalable process based on bioreactors is needed. Stem cells can be either cultured as free cells/aggregates in suspension or as adherent cells on the solid substrates. Based on the cell property, different bioreactor configurations are developed to better expand stem cells while maintaining their differentiation capacity. In this review, several major types of bioreactor systems and their applications in stem cell engineering are discussed. Continued advancements in bioprocess and bioreactor research and development are important to engineer stem cells for their use in biomedical applications.

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“…In 1967, the first microcarrier Sephadex A-50 for cell culture was developed by Van Wezel (van Wezel, 1967) using 2-diethylaminoethyl (DEAE) then, several microcarrier materials have been developed, including glucan (van Wezel, 1967), cellulose (Paris et al, 1983), chitosan (Chen et al, 2006), glucomannan (Sun et al, 2010) and polymer molecules (Reuveny et al, 1983;Gumusderelioglu et al, 2013;Zuhlke et al, 1993;Cer et al, 2007). The commercial microcarrier products in the market are GE Cytodex I, II and III, Cytopore, and Cultispher G. All these products are expensive, limits their application in the pharmaceutical industries.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of DEAE-Soybean Starch Microspheres for Adhere Animal Cell Culture

Zhang¹,

Li²,

Ma³

et al. 2017

JAS

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The present study outlines the synthesis of a new microcarrier for anchorage-dependent animal cell cultures. The new microcarriers were synthesized from the cross-linking soybean starch microspheres followed by modification with 2-diethylaminoethyl (DEAE). Furthermore, 5 g/100 mL of wet microspheres DEAE-soybean starch microspheres were applied in the adhere cell culture, with an inoculation density 2.0 × 10 5 cells/mL of BHK-21, Marc-145, and MDCK cells. The cells were shown to grow well in the DEAE-soybean starch microcarrier, with BHK-21 cells showing a higher cell density after 144 h (2.5 × 10 6 cells/mL) compared to cells grown on the commercial product Cytodex 1 (2.2 × 10 6 cells/mL). These starch microcarriers have a potential application in anchorage-dependent animal cells culture, due to its low cost and its simple process.

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Synthesis and application of new microcarriers for animal cell culture. Part I: Design of polystyrene based microcarriers

Cited by 13 publications

References 24 publications

The heterogeneous selective reduction of PHB as a useful method for preparation of oligodiols and surface modification

The heterogeneous selective reduction of PHB as a useful method for preparation of oligodiols and surface modification

Stem cell engineering in bioreactors for large‐scale bioprocessing

Synthesis of DEAE-Soybean Starch Microspheres for Adhere Animal Cell Culture

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