Synthesis and application of visual AIE fluorescent probe for lipid droplets in vivo

Wu, Lei; Li, Bin; Yan, Deyue; Zhou, Jieyu; Shi, Guangyao; Li, Yi‐Heng; Wang, Xiaoqing; Wu, Shaoping; Zhang, Yongmin; Li, Jianli

doi:10.1016/j.dyepig.2022.110946

Dyes and Pigments

2023

DOI: 10.1016/j.dyepig.2022.110946

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Synthesis and application of visual AIE fluorescent probe for lipid droplets in vivo

Lei Wu

Bin Li

Deyue Yan

et al.

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Cited by 2 publications

References 34 publications

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A Novel Fluorogenic Probe Reveals Lipid Droplet Dynamics in ME/CFS Fibroblasts

Ding,

Sanislav,

Missailidis

et al. 2024

Advanced Sensor Research

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Lipid droplets (LDs) are dynamic cellular organelles that play an essential role in lipid metabolism and storage. LD dysregulation has been implicated in various diseases. However, investigations into the cellular LD dynamics under disease conditions have been rarely reported, possibly due to the absence of high performing LD imaging agents. Here a novel fluorogenic probe, AM‐QTPA, is reported for specific LD imaging. AM‐QTPA demonstrates viscosity sensitivity and aggregation‐induced emission enhancement characteristics. It is live cell permeable and can specifically light up LDs in cells, with low background noise and superior signals that can be quantified. After validation in cell model with LD accumulation induced by oleic acid treatment, AM‐QTPA is applied in a small proof‐of‐concept number of human fibroblast samples derived from people diagnosed with myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS), a complex and debilitating disease with unknown cause. The results indicate the presence of larger but fewer LDs in ME/CFS fibroblasts compared to the healthy counterparts, accompanying with frequent LD‐mitochondria contacts, suggesting potential upregulation of lipolysis in ME/CFS connective tissue like fibroblasts. Overall, AM‐QTPA provides new understanding of the anomalous LD dynamics in disease status, which, potentially, will facilitate in‐depth investigation of the pathogenesis of ME/CFS.

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A Novel Fluorogenic Probe Reveals Lipid Droplet Dynamics in ME/CFS Fibroblasts

Ding,

Sanislav,

Missailidis

et al. 2024

Advanced Sensor Research

View full text Add to dashboard Cite

show abstract

Rational design of a two-photon probe with solvatochromic emission and AIE-activity for lipid droplet specific imaging

Bu,

Guo,

et al. 2023

Dyes and Pigments

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Synthesis and application of visual AIE fluorescent probe for lipid droplets in vivo

Cited by 2 publications

References 34 publications

A Novel Fluorogenic Probe Reveals Lipid Droplet Dynamics in ME/CFS Fibroblasts

A Novel Fluorogenic Probe Reveals Lipid Droplet Dynamics in ME/CFS Fibroblasts

Rational design of a two-photon probe with solvatochromic emission and AIE-activity for lipid droplet specific imaging

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