Synthesis and binding of new polyfluorinated aryl azides to .alpha.-chymotrypsin. New reagents for photoaffinity labeling

Abstract

The preparation of several new, water-soluble, polyfluorinated aryl azide reagents for use in photoaffinity labeling studies is described. The ability of some of these reagents and their nonfluorinated controls to noncovalently bind to alpha-chrymotrypsin is reported. It is found that polyfluorination does not interfere with ligand binding.

A Multidimensional Approach to Explore the Use of a Small Heterobifunctional Crosslinker based on a Metabolite of the Kynurenine Pathway

“…The difficulty lies in increasing the rate of intermolecular reaction. In this regard, heterobifunctional crosslinkers, based on fluorinated [20] and those based on 'long lived transients', have proved to be very useful for this purpose. This has been highlighted in recent papers from our laboratory [21][22][23].…”