Synthesis and bio-evaluation of aryl hydrazono esters for oviposition responses in Aedes albopictus

Bandyopadhyay, Prabal; Guha, Lopamudra; Seenivasagan, T.; Sathe, Manisha; Sharma, Pratibha; Parashar, B. D.; Kaushik, M. P.

doi:10.1016/j.bmcl.2010.11.101

Cited by 24 publications

(14 citation statements)

References 25 publications

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“…The need to consider the design of these bioassays for species that lay single eggs and exhibit skip oviposition has been well shown by Chadee and Corbet [ 37 ] who proposed a new study design for Aedes : one that entailed “recording the distribution of eggs by individual females initially provided with an array of identical sites”. However, their work has been ignored in consequent studies with this genus [ 38 – 42 ]. The present study therefore aimed to present new approaches for: (1) optimizing egg laying in test insects; (2) implementing tests to compare two substrates; and, (3) analysing and reporting finding of egg-count experiments.…”

Section: Introductionmentioning

confidence: 99%

Analysing the oviposition behaviour of malaria mosquitoes: design considerations for improving two-choice egg count experiments

Okal

Lindh

Torr

et al. 2015

Malar J

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BackgroundChoice egg-count bioassays are a popular tool for analysing oviposition substrate preferences of gravid mosquitoes. This study aimed at improving the design of two-choice experiments for measuring oviposition substrates preferences of the malaria vector Anopheles gambiae senso lato, a mosquito that lays single eggs.MethodsIn order to achieve high egg-laying success of female An. gambiae sensu stricto (s.s.) and Anopheles arabiensis mosquitoes in experiments, four factors were evaluated: (1) the time provided for mating; (2) the impact of cage size, mosquito age and female body size on insemination; (3) the peak oviposition time; and, (4) the host sources of blood meal. Choice bioassays, with one mosquito released in each cage containing two oviposition cups both with the same oviposition substrate (100 ml water), were used to measure and adjust for egg-laying characteristics of the species. Based on these characteristics an improved design for the egg-count bioassay is proposed.ResultsHigh oviposition rates [84%, 95% confidence interval (CI) 77–89%] were achieved when 300 male and 300 blood-fed female An. gambiae s.s. were held together in a cage for 4 days. The chances for oviposition dropped (odds ratio 0.30; 95% CI 0.14–0.66) when human host source of blood meal was substituted with a rabbit but egg numbers per female were not affected. The number of eggs laid by individual mosquitoes was overdispersed (median = 52, eggs, interquartile range 1–214) and the numbers of eggs laid differed widely between replicates, leading to a highly heterogeneous variance between groups and/or rounds of experiments. Moreover, one-third of mosquitoes laid eggs unequally in both cups with similar substrates giving the illusion of choice. Sample size estimations illustrate that it takes 165 individual mosquitoes to power bioassays sufficiently (power = 0.8, p = 0.05) to detect a 15% shift in comparative preferences of two treatments.ConclusionTwo-choice egg count bioassays with Anopheles are best done with a two-tier design that (1) implements a parallel series of experiments with mosquitoes given a choice of two identical substrates choices and, (2) uses a single mosquito in each test cage rather than groups of mosquitoes to assess the preference of a test or control solution. This approach, with sufficient replication, lowers the risk detecting pseudopreferences.

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Section: Introductionmentioning

confidence: 99%

Analysing the oviposition behaviour of malaria mosquitoes: design considerations for improving two-choice egg count experiments

Okal

Lindh

Torr

et al. 2015

Malar J

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“…Thereafter, basic hydrolysis was carried out, affording the acids 33 and 34 . During the synthesis of a series of analogs of the screened molecule, 35 , with an IC 50 value of 192 μM, an issue ensued regarding the assignment of the geometry of the C=N double bond for 31 and 32 [ 68 , 69 , 70 , 71 ]. Through a comparison of the 1 H NMR spectroscopic information for the reference molecules, the obtained materials, 31 and 32 , were confirmed to be E and Z, respectively [ 72 , 73 , 74 ].…”

Section: Resultsmentioning

confidence: 99%

Design and Synthesis of Small Molecules as Potent Staphylococcus aureus Sortase A Inhibitors

Paek

2020

Antibiotics

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The widespread and uncontrollable emergence of antibiotic-resistant bacteria, especially methicillin-resistant Staphylococcus aureus, has promoted a wave of efforts to discover a new generation of antibiotics that prevent or treat bacterial infections neither as bactericides nor bacteriostats. Due to its crucial role in virulence and its nonessentiality in bacterial survival, sortase A has been considered as a great target for new antibiotics. Sortase A inhibitors have emerged as promising alternative antivirulence agents against bacteria. Herein, the structural and preparative aspects of some small synthetic organic compounds that block the pathogenic action of sortase A have been described.

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“…Bandyopadhyay et al 13 have performed the synthesis of arylhydrazone esters (Scheme 1). Initially, it was found better attractant results in the case of unsymmetrical arylhydrazone esters than that of the symmetrical structures.…”

Section: Arylhydrazone Ester Derivativesmentioning

confidence: 99%

“…3 Additionally, mosquito control is of great importance throughout the world, especially in tropical and subtropical areas. 2,4,5,[12][13][14][15][16][17] Based on this, CHIKV can be transmitted through an urban cycle, man to mosquito to man, or through a sylvatic cycle, animal to mosquito to man. However, the use of insecticides to kill mosquitoes has led to the development of resistance.…”

Section: Introductionmentioning

confidence: 99%

The medicinal chemistry of Chikungunya virus

Silva-Júnior

Leoncini

Rodrigues

et al. 2017

Bioorganic & Medicinal Chemistry

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Arthropod-borne viruses (arboviruses) are an important threat to human and animal health globally. Among these, zoonotic diseases account for billions of cases of human illness and millions of deaths every year, representing an increasing public health problem. Chikungunya virus belongs to the genus Alphavirus of the family Togariridae, and is transmitted mainly by the bite of female mosquitoes of the Aedes aegypti and/or A. albopictus species. The focus of this review will be on the medicinal chemistry of Chikungunya virus, including synthetic and natural products, as well as rationally designed compounds.

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Synthesis and bio-evaluation of aryl hydrazono esters for oviposition responses in Aedes albopictus

Cited by 24 publications

References 25 publications

Analysing the oviposition behaviour of malaria mosquitoes: design considerations for improving two-choice egg count experiments

Analysing the oviposition behaviour of malaria mosquitoes: design considerations for improving two-choice egg count experiments

Design and Synthesis of Small Molecules as Potent Staphylococcus aureus Sortase A Inhibitors

The medicinal chemistry of Chikungunya virus

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