Synthesis and Biocompatibility of Silicone Hydrogel Functionalized with Polysaccharide

Jeong, Keun-Seung; Kim, Ho‐Joong; Hwa‐Lim, Lim; Ryu, Geun-Chang; Seo, Eun-Sun; You, Nam‐Ho; Jun, Ji Eun

doi:10.1002/bkcs.10315

Cited by 6 publications

(4 citation statements)

References 26 publications

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“…Following polymerization reaction, the hydrogel materials were soaked in milli‐Q water for 24 h for the removal of unreacted components. Swollen hydrogels were cut into discs (diameter: 5/16″, thickness: 1 mm) then dried under ambient conditions and stored at room temperature until further analysis, as done previously . Immediately prior to analysis described below, hydrogels were then incubated in phosphate‐buffered saline (PBS, pH 7.4) at room temperature for 24 h, until swollen to equilibrium.…”

Section: Methodsmentioning

confidence: 99%

Hyaluronan incorporation into model contact lens hydrogels as a built‐in lubricant: Effect of hydrogel composition and proteoglycan 4 as a lubricant in solution

et al. 2017

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Contact lens friction significantly correlates with subjective comfort. Hyaluronan (HA) and proteoglycan 4 (PRG4) are natural boundary lubricants present in the body. The objective of this study was to assess the effect of crosslinked HA into the bulk of model contact lens materials pHEMA, pHEMA/TRIS, and DMAA/TRIS on surface wettability, protein sorption, and boundary lubricating properties at a material-cornea biointerface, both alone and synergistically with PRG4 in solution. Surface wettability was assessed by water contact angle measurement, protein sorption by lysozyme sorption assay, and boundary lubricating properties using an in vitro friction test method. HA incorporation (HA ) increased the surface wettability of all materials, and reduced protein sorption for pHEMA and DMAA/TRIS. HA increased friction for pHEMA, and DMAA/TRIS, whereas a decrease was observed for pHEMA/TRIS. A combination of HA and PRG4 had a synergistic effect of reducing friction only for pHEMA/TRIS. This combination had similar friction reduction compared with PRG4 alone for DMAA/TRIS. These results indicate HA incorporation could be an effective internal wetting agent, antiadhesive, and boundary lubricant for pHEMA/TRIS silicone hydrogels. In conclusion, HA incorporation can reduce friction of hydrogels alone and in combination with PRG4 in solution, though in a hydrogel composition-dependent (e.g., TRIS) manner. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1818-1826, 2018.

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Section: Methodsmentioning

confidence: 99%

Hyaluronan incorporation into model contact lens hydrogels as a built‐in lubricant: Effect of hydrogel composition and proteoglycan 4 as a lubricant in solution

et al. 2017

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“…In addition, the surface density of lysozyme was consistently higher than the surface density of HSA (∼65%, p < 0.0001) on all samples. This was likely due to the differences in MW, conformational stability, and net charge of the two proteins, as these parameters were previously found to contribute to the protein deposition profile . When compared to surface-modified HA-grafted pHEMA hydrogels, the amount of lysozyme and HSA deposited on the these SiHy materials was lower.…”

Section: Resultsmentioning

confidence: 75%

“…This was likely due to the differences in MW, conformational stability, and net charge of the two proteins, as these parameters were previously found to contribute to the protein deposition profile. 38 When compared to surfacemodified HA-grafted pHEMA hydrogels, 50 the amount of lysozyme and HSA deposited on the these SiHy materials was lower. SiHy materials are known to attract less proteins than conventional hydrogel CLs.…”

Section: ■ Results and Discussionmentioning

confidence: 97%

“…The incorporation of HA into the bulk of model SiHy materials either as an internal or a releasable wetting agent or in the structure of an interpenetrating network led to enhanced wettable surfaces, which not only showed reduced nonspecific protein deposition − but also reduced lysozyme denaturation . Surface immobilization of CLs with HA binding peptides was found to locally attract and concentrate exogenous HA, creating a thin HA coating that is able to retain moisture on the surface of the material .…”

Section: Introductionmentioning

confidence: 99%

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Impact of a Hyaluronic Acid-Grafted Layer on the Surface Properties of Model Silicone Hydrogel Contact Lenses

2018

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The introduction of high oxygen transmissibility silicone hydrogel lenses ameliorated hypoxia-related complications, making them the most prescribed type of contact lens (CL). Despite the progress made over the last 2 decades to improve their clinical performance, symptoms of ocular dryness and discomfort and a variety of adverse clinical events are still reported. Consequently, the rate of CL wear discontinuation has not been appreciably diminished by their introduction. Aiming to improve the interfacial interactions of silicone hydrogel CLs with the ocular surface, a biomimetic layer of hydrophilic glycosaminoglycan hyaluronic acid (HA) (100 kDa) was covalently attached to the surface of model poly(2-hydroxyethyl methacrylate-co-3-methacryloxypropyl-tris-(trimethylsiloxy)silane) (pHEMA-co-TRIS) silicone hydrogel materials via UV-induced thiol-ene “click” chemistry. The surface structural changes after each modification step were studied by Fourier transform infrared spectroscopy–attenuated total reflectance and X-ray photoelectron spectroscopy (XPS). Successful grafting of a homogeneous HA layer to the surface of the model silicone hydrogels was confirmed by the consistent appearance of N (1s) and the significant decrease of the Si (2p) peaks, as determined by low-resolution angle-resolved XPS. The HA-grafted surfaces demonstrated reduced contact angles, dehydration rate, and nonspecific deposition of lysozyme and albumin, while maintaining their optical transparency (>90%). In vitro studies demonstrated that the HA-grafted pHEMA-co-TRIS materials did not show any toxicity to human corneal epithelial cells. These results suggest that surface immobilization of HA via thiol-ene “click” chemistry can be used as a promising surface treatment for silicone hydrogel CLs.

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Multi-Responsive Hydrogels Functionalized with a Photochromic Spiropyran-Conjugated Chitosan Network

2018

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Synthesis and Biocompatibility of Silicone Hydrogel Functionalized with Polysaccharide

Cited by 6 publications

References 26 publications

Hyaluronan incorporation into model contact lens hydrogels as a built‐in lubricant: Effect of hydrogel composition and proteoglycan 4 as a lubricant in solution

Hyaluronan incorporation into model contact lens hydrogels as a built‐in lubricant: Effect of hydrogel composition and proteoglycan 4 as a lubricant in solution

Impact of a Hyaluronic Acid-Grafted Layer on the Surface Properties of Model Silicone Hydrogel Contact Lenses

Multi-Responsive Hydrogels Functionalized with a Photochromic Spiropyran-Conjugated Chitosan Network

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