2007
DOI: 10.1002/chin.200728105
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Synthesis and Biological Evaluation of Dialkylsubstituted Maleic Anhydrides as Novel Inhibitors of Cdc25 Dual Specificity Phosphatases.

Abstract: Phosphatases. -10 Dialkyl-substituted maleic anhydrides of type (VII) are prepared and tested as novel inhibitors of Cdc25 dual specificity phosphatases. The optimum activity is reached with a C17 chain length as substituent R. -(BRAULT, L.; DENANCE, M.; BANASZAK, E.; EL MAADIDI, S.; BATTAGLIA, E.; BAGREL, D.; SAMADI*, M.; Eur.

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Cited by 7 publications
(11 citation statements)
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“…Recombinant CDC25-GST proteins of the three isoforms were obtained as previously described by Brault et al [26]. Briefly, E. coli strain BL21-DE3 pLys S was transformed by a plasmidic vector (pGEX 2T, Amersham, GE Healthcare, V elizy, France) containing the sequences encoding full length CDC25A, B, and C or only the catalytic site of CDC25B.…”
Section: Production and Purification Of Recombinant Human Cdc25 Phospmentioning
confidence: 99%
“…Recombinant CDC25-GST proteins of the three isoforms were obtained as previously described by Brault et al [26]. Briefly, E. coli strain BL21-DE3 pLys S was transformed by a plasmidic vector (pGEX 2T, Amersham, GE Healthcare, V elizy, France) containing the sequences encoding full length CDC25A, B, and C or only the catalytic site of CDC25B.…”
Section: Production and Purification Of Recombinant Human Cdc25 Phospmentioning
confidence: 99%
“…Human glutathione‐ S ‐transferase (GST)‐Cdc25 recombinant enzymes were used to evaluate the inhibitory potential of the compounds. Each isoenzyme was prepared as described previously 19,20. Briefly, the GST‐tagged Cdc25s were expressed in a bacterial expression system through isopropyl β‐ D ‐1‐thiogalactopyranoside induction.…”
Section: Resultsmentioning
confidence: 99%
“…For each compound, each concentration was separately tested in 3 independent microplates, at the rate of 3 wells per microplate. The statistical evaluation of IC 50 was made with software specially designed for calculating the median inhibitory concentration for toxicity tests 20…”
Section: Resultsmentioning
confidence: 99%
“…Recently, a series of maleic anhydrides bearing various simple alkyl side chains at C-4 position have been evaluated [61]. Recently, a series of maleic anhydrides bearing various simple alkyl side chains at C-4 position have been evaluated [61].…”
Section: Compounds Acting As Phosphate Mimicsmentioning
confidence: 99%