1991
DOI: 10.1080/07328319108046455
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Synthesis and Biophysical and Biological Evaluation of 2′-Modified Antisense Oligonucleotides

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Cited by 34 publications
(20 citation statements)
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“…Oligonucleotide Synthesis-2Ј-alkoxy and 2Ј-fluoro monomers were synthesized as described previously (31,32). Synthesis of phosphorothioate and phosphodiester oligonucleotides (deoxy and 2Ј-modified) were performed using an Applied Biosystems 380B automated DNA synthesizer as described previously (27).…”
Section: Methodsmentioning
confidence: 99%
“…Oligonucleotide Synthesis-2Ј-alkoxy and 2Ј-fluoro monomers were synthesized as described previously (31,32). Synthesis of phosphorothioate and phosphodiester oligonucleotides (deoxy and 2Ј-modified) were performed using an Applied Biosystems 380B automated DNA synthesizer as described previously (27).…”
Section: Methodsmentioning
confidence: 99%
“…Purification of oligonucleotides was also as described previously (29,30). Purified oligonucleotides were greater than 90% full-length material as determined by capillary gel electrophoretic analysis.…”
Section: Materials-t4 Polynucleotide Kinase Was Purchased From Promegmentioning
confidence: 99%
“…Oligonucleotide Synthesis-Synthesis of 2Ј-methoxy, 2Ј-fluoro, 2Ј-propoxy, and deoxy chimeric oligonucleotides was performed using an Applied Biosystems 380B automated DNA synthesizer as described previously (29,30). Purification of oligonucleotides was also as described previously (29,30).…”
Section: Materials-t4 Polynucleotide Kinase Was Purchased From Promegmentioning
confidence: 99%
“…These site-specific cleavage rates (V 0/s ) were measured by quantitating the amount of RNase H digestion for each individual cleavage site as a function of time. For example, site-specific cleavage rates were determined for residues G 26 , G 27 , A 29 , and G 31 of the 22-13 pseudo-half-knot substrate (Fig. 2, A and B).…”
mentioning
confidence: 99%