Synthesis and catalytic evaluation of Fe₃O₄/MWCNTs nanozyme as recyclable peroxidase mimetics: Biochemical and physicochemical characterization

Abstract

In this research, the nanocomposite of multiwalled carbon nanotubes and magnetic metal oxide nanoparticles (Fe3O4/MWCNTs), as enzyme mimetic, was synthesized using an in situ chemical reduction method. The structure, composition and morphology of the prepared Fe3O4/MWCNT nanocomposite materials were characterized using X‐ray diffraction, FT‐IR and scanning electron microscopy with energy dispersive X‐ray spectroscopy, respectively. The magnetic properties of the nanocomposite were investigated by the vibrating sample magnetometer. A colorimetric system involving nanozyme, phenol/4‐aminoantipyrine and H2O2 was utilized for the determination of peroxidase mimetic catalytic assay. The obtained results confirmed that the synthesis of Fe3O4/MWCNTs nanostructures was successful. It was found that Fe3O4/MWCNTs nanohybrid exhibited peroxidase‐like activity without any pH limitation. Colorimetric data demonstrated that the prepared nanocatalyst had higher catalytic activity toward H2O2 than MWCNTs. The kinetic parameters of the nanozyme, Km and Vmax, were estimated to be 8.3 mm and 1.4 mm min−1, respectively. The Fe3O4/MWCNTs nanostructures were also successfully applied for glucose detection. In addition, peroxidase‐like activity of the nanozyme increased in the presence of butyl‐imidazolium bromide ionic liquid. These biomimetic catalysts have some advantages, such as simplicity, stability, reusability and cost effectiveness, which makes them great candidates to be used in various fields of biotechnology applications.

Recent Advances in Nanozyme-Mediated Strategies for Pathogen Detection and Control

“…Their robust detection capabilities and potential for future-oriented applications make them particularly attractive for utilization in this sector [43]. In contrast to their natural enzyme counterparts, nanozymes possess several advantageous features including cost effectiveness [44], facile scalability for mass production [45], resilience towards harsh environmental conditions [46], prolonged storage capability [47], exceptional stability, and the ability to customize their activities as per specific requirements [45]. Nanozymes have demonstrated tremendous potential in surpassing the limitations of conventional enzymes within the field of analyses.…”

Single‐Atom Nanozymes for Biomedical Applications: Recent Advances and Challenges

“…As glucose and oxygen react enzymatically to create H 2 O 2 , nanozymes with peroxidase activity can then be applied to indirectly determine glucose levels [69] . Such as MoS 2 nanosheets/N‐atom‐doped graphene oxide, [70] Fe 3 O 4 /MW carbon nanotubes (CNTs), [71] etc., whereas SAzymes as more active nanozymes have also been investigated for application in more sensitive assays of glucose. Min Chen group [14] designed a single iron site nanozymes (Fe‐SSN) by a sacrificial carrier approach, where the porous structure of nitrogen‐doped carbon allows for a more dispersed but guaranteed number of iron sites to achieve higher peroxidase activity.…”

Perylene diimide‐modified magnetic γ‐Fe2O3/CeO2 nanoparticles as peroxidase mimics for highly sensitive colorimetric detection of Vitamin C

“…In the past decade, the synthesis of magnetic iron oxide nanoparticles has been intensively developed not only for magnetic storage media but also for biosensing applications . To achieve a better performance, the magnetic bases were always decorated by other highly active substrates to realize a synergistic effect, such as Fe 3 O 4 @SiO 2 nanoparticles, Fe 3 O 4 /CeO 2 composite, Fe 3 O 4 /nitrogen‐doped carbon hybrid nanofibers, prussian blue‐modified Fe 2 O 3 nanoparticles, Fe 3 O 4 ‐Pt/core‐shell nanoparticles, and carbon dots/Fe 3 O 4 hybrid nanofibers .…”