Synthesis and characterisation of copper acetate mediated oxidative coupled product of naturally occurring hydroxy benzoquinone, embelin and its in vitro and in vivo cytotoxic studies in Dalton Lymphoma ascites tumour cells

Abstract

Embelin is an active phytoconstituent known to exhibit a variety of biological activities, especially active against various cancer and tumour cell lines. In the present work, embelin was extracted and isolated from Embelia ribes and was structurally modified by incorporating different fluoro substituted aniline in the quinone motif with a view of enhancing the biological activity. The synthesis was carried out in presence of copper acetate catalyst in a protic solvent, glacial acetic acid to obtain EDFA, ETFA and EOCF and were characterized by various spectral techniques. Embelin and its derivatives were then subjected to in vitro studies in DLA cell lines. Antiangeogenic activities were tested using CAM assay. EOCF was identified as the most active derivative and hence subjected to in vivo studies in tumor induced albino mice. The activity was compared with currently used anticancer drug, cyclophosphamide. The study revealed that EOCF was effective in inhibiting tumour growth.