Synthesis and characterisation of doxorubicin-loaded functionalised cobalt ferrite nanoparticles and their &lt;i&gt;in vitro&lt;/i&gt; anti-tumour activity under an AC-magnetic field

Mushtaq, Muhammad; Kanwal, Farah; Islam, Atif; Ahmed, Khalil; Haq, Ziaul; Jamil, Tahir; Imran, Muhammad; Abbas, Syed Mustansar

doi:10.4314/tjpr.v16i7.27

Cited by 13 publications

(4 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Spinel cobalt ferrites (CoFe 2 O 4 ) MNPs are being extensively investigated and have been proposed for biomedical applications [14,15]. Nanoparticles of CoFe 2 O 4 are of great importance for their inimitable optical, magnetic, electronic and physical properties which include large magnetocrystalline anisotropy, large magnetostrictive coefficient, high Curie temperature and exceptional chemical stability [16].…”

Section: Introductionmentioning

confidence: 99%

Aminodextran Coated CoFe2O4 Nanoparticles for Combined Magnetic Resonance Imaging and Hyperthermia

et al. 2020

View full text Add to dashboard Cite

Aminodextran (AMD) coated magnetic cobalt ferrite nanoparticles are synthesized via electrostatic adsorption of aminodextran onto magnetic nanoparticles and their potential theranostic application is evaluated. The uncoated and aminodextran-coated nanoparticles are characterized to determine their hydrodynamic size, morphology, chemical composition, zeta potential and magnetization. The aminodextran containing cobalt ferrite nanoparticles of nanometer size are positively charged in the pH range from 3 to 9 and exhibit saturation magnetization of 50 emu/g. The magnetic resonance imaging (MRI) indicates capability for diagnostics and a reduction in intensity with an increase in nanoparticle amount. The hyperthermia capability of the prepared particles shows their potential to generate suitable local heat for therapeutic purposes. There is a rise of 7 °C and 9 °C at 327 kHz and 981 kHz respectively and specific absorption rates (SAR) of aminodextran-coated nanoparticles are calculated to be 259 W/g and 518 W/g at the given frequencies larger than uncoated nanoparticles (0.02 W/g). The development of novel aminodextran coated magnetic cobalt ferrite nanoparticles has significant potential to enable and improve personalized therapy regimens, targeted cancer therapies and ultimately to overcome the prevalence of nonessential and overdosing of healthy tissues and organs.

show abstract

Section: Introductionmentioning

confidence: 99%

Aminodextran Coated CoFe2O4 Nanoparticles for Combined Magnetic Resonance Imaging and Hyperthermia

et al. 2020

View full text Add to dashboard Cite

show abstract

“…However, magnetic NPs present inherent tendencies to aggregate, which results in short-term stability in biological suspensions [11]- [13]. Hence, modifications of the NPs using polymers such as polyethylene glycol (PEG) and chitosan (CS) often resolve these challenges [14]. Recently, there have been reports on the synthesis of biocompatible and stable polymercoated magnesium-doped ferrite derivatives using PEG, PVA, and chitosan, and showed their potential in the delivery of the anti-cancer drugs, doxorubicin and 5-fluorouracil [15]- [17].…”

Section: Introductionmentioning

confidence: 99%

Design and synthesis of biocompatible magnetic ferrite nanoparticles conjugated with Angiopep for LRP-1 receptor-mediated delivery

MOKHOSI,

MDLALOSE,

SINGH

2023

NANOCON Conference Proeedings

View full text Add to dashboard Cite

Disorders and diseases of the brain present an enormous challenge due to the physical and physiological properties of the blood-brain barrier (BBB), making entry for therapeutics difficult. In recent years, increased research has focused on using magnetic ferrite nanoparticles (NPs) in various biomedical applications. In this study, we report on the design of ferrite NPs for traversing the BBB. Core ferrite CoFe2O4 and Mg0.5Co0.5Fe2O4 NPs were synthesized using the glycol-thermal route and coated with chitosan (CS). A BBB-targeting peptide, Angiopep-2 (ANG), was covalently conjugated to the coated NPs. The physical, chemical, and magnetic properties of the core and derivative ferrite NPs were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), high-resolution transmission electron microscopy (HR-TEM), nanoparticle tracking analysis (NTA) and vibrating sample magnetometer (VSM). Docking studies were conducted using Autodock Vina software for binding. XRD, HR-TEM and VSM results revealed spinel crystalline ferrite core NPs exhibiting superparamagnetic behaviour. Particle core sizes of the ferrite NPs were below 13.6 nm. The FTIR spectra showed characteristic peak shifts upon CS-coating and conjugation with Angiopep-2, thus confirming functionalization. Hydrodynamic sizes of 90.4 nm and 152. nm were reported for the ANG-CS-CoFe2O4, and ANG-CS-Mg0.5Co0.5Fe2O4, respectively. ANG-CS-Mg0.5Co0.5Fe2O4 presented the most stability with a zeta potential (+29.0 mV), while ANG-CS-CoFe2O4 has +19.2 mV. Molecular docking investigations revealed binding energies for the CS-conjugated ANG of -5.67 kCal/mol, suggesting that the LRP-1 receptor was not significantly hindered. These NPs exhibit potential for targeted CNS delivery and can be explored for in vitro gene and drug delivery.

show abstract

“…Furthermore, in order to be exploited in biomedical applications, NPs need to fulfill certain criteria which include water solubility, excellent colloidal stability, biocompatibility, and high saturation magnetization which enables controlled and nontoxic biological interactions [ 18 ]. The hydrophilicity of the nanocarriers is important, as native hydrophobic surfaces of NPs are rapidly opsonized by hydrophobic serum proteins [ 19 ]. For this, surface functionalization has a major role [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Biocompatibility and cytotoxicity in vitro of surface-functionalized drug-loaded spinel ferrite nanoparticles

Mushtaq¹,

Shahzad²,

Saeed³

et al. 2021

Beilstein J. Nanotechnol.

View full text Add to dashboard Cite

In this study, poly(isobutylene-alt-maleic anhydride) (PMA)-coated spinel ferrite (MFe2O4, where M = Fe, Co, Ni, or Zn) nanoparticles (NPs) were developed as carriers of the anticancer drugs doxorubicin (DOX) and methotrexate (MTX). Physical characterizations confirmed the formation of pure cubic structures (14–22 nm) with magnetic properties. Drug-loaded NPs exhibited tumor specificity with significantly higher (p < 0.005) drug release in an acidic environment (pH 5.5). The nanoparticles were highly colloidal (zeta potential = −35 to −26 mV) in deionized water, phosphate buffer saline (PBS), and sodium borate buffer (SBB). They showed elevated and dose-dependent cytotoxicity in vitro compared to free drug controls. The IC50 values ranged from 0.81 to 3.97 μg/mL for HepG2 and HT144 cells, whereas IC50 values for normal lymphocytes were 10 to 35 times higher (18.35–43.04 µg/mL). Cobalt ferrite (CFO) and zinc ferrite (ZFO) NPs were highly genotoxic (p < 0.05) in cancer cell lines. The nanoparticles caused cytotoxicity via oxidative stress, causing DNA damage and activation of p53-mediated cell cycle arrest (significantly elevated expression, p < 0.005, majorly G1 and G2/M arrest) and apoptosis. Cytotoxicity testing in 3D spheroids showed significant (p < 0.05) reduction in spheroid diameter and up to 74 ± 8.9% of cell death after two weeks. In addition, they also inhibited multidrug resistance (MDR) pump activity in both cell lines suggesting effectivity in MDR cancers. Among the tested MFe2O4 NPs, CFO nanocarriers were the most favorable for targeted cancer therapy due to excellent magnetic, colloidal, cytotoxic, and biocompatible aspects. However, detailed mechanistic, in vivo cytotoxicity, and magnetic-field-assisted studies are required to fully exploit these nanocarriers in therapeutic applications.

show abstract

Synthesis and characterisation of doxorubicin-loaded functionalised cobalt ferrite nanoparticles and their <i>in vitro</i> anti-tumour activity under an AC-magnetic field

Cited by 13 publications

References 21 publications

Aminodextran Coated CoFe2O4 Nanoparticles for Combined Magnetic Resonance Imaging and Hyperthermia

Aminodextran Coated CoFe2O4 Nanoparticles for Combined Magnetic Resonance Imaging and Hyperthermia

Design and synthesis of biocompatible magnetic ferrite nanoparticles conjugated with Angiopep for LRP-1 receptor-mediated delivery

Biocompatibility and cytotoxicity in vitro of surface-functionalized drug-loaded spinel ferrite nanoparticles

Contact Info

Product

Resources

About