Synthesis and characterization of a lipase-friendly DES based on cholinium dihydrogen phosphate

Álvarez, María S.; Longo, María A.; Deive, Francisco J.; Rodríguez, Ana

doi:10.1016/j.molliq.2021.117230

Cited by 5 publications

(3 citation statements)

References 43 publications

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“…In the present study, the presence of 10% v/v EAC-based DESs resulted in diminished hydrolytic activity compared to the results of another work [44]. In the case of the Chol DHP-based DESs, Álvarez et al studied the activity of CALB in the hydrolysis of p-nitrophenyl laurate in such DESs and found it to be >60% at the concentration of 10% v/v [45].…”

Section: Effect Of Dess On the Activity Of Immobilized Calbsupporting

confidence: 38%

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Development of 3D Printed Enzymatic Microreactors for Lipase-Catalyzed Reactions in Deep Eutectic Solvent-Based Media

et al. 2022

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In this study, 3D printing technology was exploited for the development of immobilized enzyme microreactors that could be used for biocatalytic processes in Deep Eutectic Solvent (DES)-based media. 3D-printed polylactic acid (PLA) microwell plates or tubular microfluidic reactors were modified with polyethylenimine (PEI) and lipase from Candida antarctica (CALB) was covalently immobilized in the interior of each structure. DESs were found to have a negligible effect on the activity and stability of CALB, and the system proved highly stable and reusable in the presence of DESs for the hydrolysis of p-nitrophenyl butyrate (p-NPB). A kinetic study under flow conditions revealed an enhancement of substrate accessibility in the presence of Betaine: Glycerol (Bet:Gly) DES, while the system was not severely affected by diffusion limitations. Incubation of microreactors in 100% Bet:Gly preserved the enzyme activity by 53% for 30 days of storage at 60 °C, while the buffer-stored sample had already been deactivated. The microfluidic enzyme reactor was efficiently used for the trans-esterification of ethyl ferulate (EF) with glycerol towards the production of glyceryl ferulate (GF), known for its antioxidant potential. The biocatalytic process under continuous flow conditions exhibited 23 times higher productivity than the batch reaction system. This study featured an effective and robust biocatalytic system with immobilized lipase that can be used both in hydrolytic and synthetic applications, while further optimization is expected to upgrade the microreactor system performance.

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Section: Effect Of Dess On the Activity Of Immobilized Calbsupporting

confidence: 38%

“…CALB in the hydrolysis of p-nitrophenyl laurate in such DESs and found it to be >60% at the concentration of 10% v/v [45].…”

Section: Effect Of Dess On the Activity Of Immobilized Calbmentioning

confidence: 99%

Development of 3D Printed Enzymatic Microreactors for Lipase-Catalyzed Reactions in Deep Eutectic Solvent-Based Media

et al. 2022

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“…At the same time, the amino acid residues on the surface of CALB can form hydrogen bonds with DESs to stabilize the secondary structure of the enzyme and enhance the stability of lipases. Álvarez et al [ 83 ] concluded that the activity level of CALB in glycol-based DESs was higher than that in other DESs, which may be due to the fact that the hydrogen bonds formed between these types of solvents and CALB are weaker and do not result in a structural change that causes the loss of lipase activity. Coincidentally, Hollenbach et al [ 84 ] found that the ester exchange activity of lipases in urea-based DESs was significantly higher than that of glucose-based DESs due to similar reasons.…”

Section: Effect Of Different Solvents On Stability and Activity Of Li...mentioning

confidence: 99%

Solvent Tolerance Improvement of Lipases Enhanced Their Applications: State of the Art

Chen,

Jin,

Nian

et al. 2024

Molecules

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Lipases, crucial catalysts in biochemical synthesis, find extensive applications across industries such as food, medicine, and cosmetics. The efficiency of lipase-catalyzed reactions is significantly influenced by the choice of solvents. Polar organic solvents often result in a decrease, or even loss, of lipase activity. Conversely, nonpolar organic solvents induce excessive rigidity in lipases, thereby affecting their activity. While the advent of new solvents like ionic liquids and deep eutectic solvents has somewhat improved the activity and stability of lipases, it fails to address the fundamental issue of lipases’ poor solvent tolerance. Hence, the rational design of lipases for enhanced solvent tolerance can significantly boost their industrial performance. This review provides a comprehensive summary of the structural characteristics and properties of lipases in various solvent systems and emphasizes various strategies of protein engineering for non-aqueous media to improve lipases’ solvent tolerance. This study provides a theoretical foundation for further enhancing the solvent tolerance and industrial properties of lipases.

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New natural deep eutectic solvent for vortex-assisted liquid–liquid microextraction for the determination of bisphenols in beverages

Li,

Lai

et al. 2024

Microchemical Journal

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Synthesis and characterization of a lipase-friendly DES based on cholinium dihydrogen phosphate

Cited by 5 publications

References 43 publications

Development of 3D Printed Enzymatic Microreactors for Lipase-Catalyzed Reactions in Deep Eutectic Solvent-Based Media

Development of 3D Printed Enzymatic Microreactors for Lipase-Catalyzed Reactions in Deep Eutectic Solvent-Based Media

Solvent Tolerance Improvement of Lipases Enhanced Their Applications: State of the Art

New natural deep eutectic solvent for vortex-assisted liquid–liquid microextraction for the determination of bisphenols in beverages

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