Synthesis and characterization of Ag-containing calcium phosphates with various Ca/P ratios

Gokcekaya, Ozkan; Ueda, Kyosuke; Narushima, Takayuki; Ergun, Celaletdin

doi:10.1016/j.msec.2015.04.025

Cited by 36 publications

(33 citation statements)

References 40 publications

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“…Besides, specific surface area has a significant promotion on cell adhesion and proliferation, as well as osteogenic differentiation. The larger the specific surface area of the material is, the greater the amount of protein it can adsorb [32][33][34][35]. As a consequence, the as-described properties of -CO 3 2 -containing BCP is expected to be a desirable candidate for biomedical application.…”

Section: Resultsmentioning

confidence: 99%

Design and construction of a microporous CO 3 2 − -containing HA/β-TCP biphasic ceramic as a novel bone graft material

Dou

Chen

et al. 2020

Mater. Res. Express

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Biphasic calcium phosphate (BCP) ceramics, viewed as the first-line therapeutic materials in clinical practice, are still confronted with challenges such as undesirable biocompatibility and bioactivity given their structural and chemical deficiency. In this study, we designed a novel microporous -CO 3 2 -containing BCP ceramic from Salmo salar bone in a CO 2 atmosphere via two-step hightemperature sintering, and its physicochemical properties and biocompatibility were explored. A without -CO 3 2 -BCP bioceramic material prepared in a single gas atmosphere was used as a comparison. The two scaffolds were characterized by x-ray diffraction (XRD), fourier transform infrared (FTIR) spectroscopy, and scanning electron microscopy (SEM) , measured the size of its porosity and specific surface area by an instrument and in vitro cell experiment was used to test its biocompatibility. Such synthetic strategy endowed the BCP products with increased β-tricalcium phosphate (β-TCP) percentage from 37.18wt% to 45.3wt% (compared to BCP without -CO 3 2 ), with the -CO 3 2 content calculated to be 1.98wt%, which is close to that in human bone tissue. The amount of -CO 3 2 incorporation, the a-axes of the lattice parameters have decreased significantly from 9.4845 nm to 9.2942 nm by Rietveld structure refinement. Especially, the novel -CO 3 2 containing BCP exhibits a lower degree of crystallinity than that without CO3 2-. In addition, the natural microporosity remarkably increased from 49.6% for BCP without -CO 3 2 to 64.06% for -CO 3 2 -containing BCP, and the specific surface area increased from 5.829 m 2 g −1 to 17.161 m 2 g −1 . Besides, two materials were non-toxic to human bone marrow mesenchymal stem cells (hBMSCs), and the -CO 3 2 -containing BCP had more factor expression in the early stage of osteogenesis induction, such as Runx2 and Smad 4. Overall, microporous -CO 3 2 -containing BCP were prepared via a simple preparation method from natural bone, combining the structural and chemical preponderance, as well as in vitro performance it should be considered as a promising biomaterial for further explorations in the fields of bone graft materials from natural bone to satisfy versatile clinical requirements.

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Section: Resultsmentioning

confidence: 99%

Design and construction of a microporous CO 3 2 − -containing HA/β-TCP biphasic ceramic as a novel bone graft material

Dou

Chen

et al. 2020

Mater. Res. Express

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“…Rameshbabu et al, used microwave radiation and fabricated composites of Ag 3 (PO 4 ) and HA, wherein the Ag‐doping content is above three atomic percentage (Rameshbabu et al, ). Similarly, Ag 3 (PO 4 ) was formed when Ag‐doped CaP powders were coprecipitated at room temperature from all the varying Ca/P (1.33–1.67) solutions containing the highest silver nitrate concentration (Gokcekaya, Ueda, Narushima, & Ergun, ). The unstable phase of Ag 3 (PO 4 ) finally transformed into stable Ag when the as‐synthesized powders were exposed to high sintering temperatures.…”

Section: Discussionmentioning

confidence: 99%

“…▲ indicates statistically significant with respect to third exchange. CFU, colony forming units; NB, newberyite nitrate concentration (Gokcekaya, Ueda, Narushima, & Ergun, 2015).…”

Section: Discussionmentioning

confidence: 99%

Silver (Ag) doped magnesium phosphate microplatelets as next‐generation antibacterial orthopedic biomaterials

et al. 2019

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This article reports for the first time our successful result in the synthesis of antibacterial single‐phase newberyite (NB, MgHPO4.3H2O), an important magnesium phosphate (MgP) bioceramic. The prime novelty lies in the fact that we explore novel MgPs as next‐generation orthopedic biomaterials as opposed to conventional calcium phosphates (CaP). While NB has already shown great promise, unlike its competitor struvite (ST, MgNH4PO4.6H2O), NB is not intrinsically antibacterial. Given the havoc created by surgical site infections (SSI) in orthopedics, it would be worthwhile to explore if antibacterial NB can be synthesized cost‐effectively. To accomplish that central goal, we used silver ion (Ag+) containing precursor solutions and exposed those to microwave irradiation. This action resulted in the rapid synthesis of NB microplatelets. Besides, three other specific objectives are addressed. First, Ag‐doping was optimized to preserve the single‐phase nature for sustained dopant release. Second, Ag+ release kinetics against common infection causing bacterial strains was analyzed. Finally, we inspected for any harmful effect of Ag‐doped NB on MC3T3 preosteoblasts. Interestingly, the single‐phase nature of NB microplatelets can be retained until 2 wt % Ag‐doping and they exhibit good antibacterial and cytocompatible properties. Even though 3 wt % Ag‐doped compositions (composites) were 100% antibacterial; they were cytotoxic.

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“…Previous studies have reported that, during the solution co‐precipitation process, Ag 3 PO 4 , NH 4 H 2 PO 4 and other impurities were often detected as minor phases in the synthesized calcium phosphate . Specifically, for calcium phosphate co‐precipitated from solution with pH values higher than 4.2, hydroxyapatite (HA) rather than other calcium phosphates such as tricalcium phosphate (TCP) was usually found as the main phase .…”

Section: Discussionmentioning

confidence: 99%

“…Silver‐doped‐calcium phosphate (silver‐CaP) powders were synthesized using a solution co‐precipitation method modified from previous reports . Among the different methods to prepare calcium phosphate, the solution precipitation approach was used in this study due to its low cost and ease of application for industrial production .…”

Section: Methodsmentioning

confidence: 99%

Industrial‐scale fabrication of an osteogenic and antibacterial PLA/silver‐loaded calcium phosphate composite with significantly reduced cytotoxicity

2018

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In this study, we report an industrial-scale fabrication method of a multifunctional polymer composite as a scaffold material for bone tissue engineering. This study successfully demonstrated the potential of applying industrial polymer processing technologies to produce specially functionalized tissue engineering scaffolds. With the inclusion of a newly synthesized multifunctional additive, silver-doped-calcium phosphate (silver-CaP), the composite material exhibited excellent osteogenic inducibility of human adipose-derived stem cells (hASC) and satisfactory antibacterial efficacy against Escherichia coli and Staphylococcus aureus. Also, relative to previously reported methods of direct loading silver particles into polymeric materials, our composite exhibited significantly reduced silver associated cytotoxicity. The enhanced biocompatibility could be a significant advantage for materials to be used for regenerative medicine applications where clinical safety is a major consideration. The impact of different silver loading methodologies on hASC' osteogenic differentiation was also studied. Overall, the results of this study indicate a promising alternative approach to produce multifunctional scaffolds at industrial-scale with higher throughput, lower cost, and enhanced reproducibility. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res B Part B: Appl Biomater, 2018.

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Synthesis and characterization of Ag-containing calcium phosphates with various Ca/P ratios

Cited by 36 publications

References 40 publications

Design and construction of a microporous CO 3 2 − -containing HA/β-TCP biphasic ceramic as a novel bone graft material

Design and construction of a microporous CO 3 2 − -containing HA/β-TCP biphasic ceramic as a novel bone graft material

Silver (Ag) doped magnesium phosphate microplatelets as next‐generation antibacterial orthopedic biomaterials

Industrial‐scale fabrication of an osteogenic and antibacterial PLA/silver‐loaded calcium phosphate composite with significantly reduced cytotoxicity

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