2024
DOI: 10.3390/ijms25052897
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Synthesis and Characterization of Amine and Aldehyde-Containing Copolymers for Enzymatic Crosslinking of Gelatine

Silvana Alfei,
Federica Pintaudi,
Guendalina Zuccari

Abstract: In tissue engineering (TE), the support structure (scaffold) plays a key role necessary for cell adhesion and proliferation. The protein constituents of the extracellular matrix (ECM), such as collagen, its derivative gelatine, and elastin, are the most attractive materials as possible scaffolds. To improve the modest mechanical properties of gelatine, a strategy consists of crosslinking it, as naturally occurs for collagen, which is stiffened by the oxidative action of lysyl oxidase (LO). Here, a novel protoc… Show more

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Cited by 1 publication
(14 citation statements)
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“…When implanted, the scaffold should ideally resist for the time necessary for the growth and remodulation of the new tissue and then should degrade; it should be resorbed and replaced by the proteins produced by the same cells growing on it [7]. Figure 1, from our recent paper, schematically summarizes the main steps of TE reported here [8]. Using TE, it is possible to have available effective substitutes for the liver, kidneys, and pancreas, as well as for tissues such as blood vessels, skin, cartilage, bones, ligaments, and tendons.…”
Section: Introductionmentioning
confidence: 98%
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“…When implanted, the scaffold should ideally resist for the time necessary for the growth and remodulation of the new tissue and then should degrade; it should be resorbed and replaced by the proteins produced by the same cells growing on it [7]. Figure 1, from our recent paper, schematically summarizes the main steps of TE reported here [8]. Using TE, it is possible to have available effective substitutes for the liver, kidneys, and pancreas, as well as for tissues such as blood vessels, skin, cartilage, bones, ligaments, and tendons.…”
Section: Introductionmentioning
confidence: 98%
“…In this context, collagen is naturally stabilized and strengthened by crosslinking reactions through the lysyl oxidase (LO)-assisted oxidation of the amino groups of its lysine and hydroxylysine residues to aldehyde groups. Subsequent aldolic condensation reactions or imine bond formations between the oxidized residues or between the oxidized groups and amine residues, respectively (Scheme 1, in Alfei et al, 2024), lead to a mechanically improved reticulated framework [8].…”
Section: Introductionmentioning
confidence: 99%
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