Synthesis and characterization of cerium, silver and copper oxide nanoparticles and their anticancer potential of hepatocellular carcinoma HepG2 cancer cells

Younas, Madiha; Zubaır, Muhammad; Rizwan, Muhammad; Khan, Muhammad Asaf; Hussaini, Khalid Mahmud; Mumtaz, Rabia; Azeem, Muhammad; Abbas, Tahir; Irshad, Muhammad; Ali, Shafaqat

doi:10.1016/j.molstruc.2023.135756

Cited by 10 publications

(1 citation statement)

References 52 publications

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“… 22 The anticancer potency of ZnO NPs is mainly through zinc-mediated protein activity disequilibrium and oxidative stress. 23 Recently, it has been reported that the enhanced anticancer efficacy of CuO NPs is against breast cancer, 24 hepatocellular carcinoma, 25 lung cancer, 26 and cervical cancer. 27 The anticancer mechanism of CuO NPs is mainly through genotoxicity and apoptotic death in cancerous cells due to their ability to generate enhanced ROS.…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis of Artemisia abyssinica Leaf Extract-Mediated Bimetallic ZnO–CuO Nanoparticles: Antioxidant, Anticancer, and Molecular Docking Studies

Orshiso,

Zereffa,

Murthy

et al. 2023

ACS Omega

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Currently, plant extract-mediated synthesized metal oxide nanoparticles (MO NPs) have played a substantial role in biological applications. Hence, this study focused on the eco-benign one-pot synthesis of bimetallic ZnO−CuO nanoparticles (ZC NPs) using the leaf extract of Artemisia abyssinica (LEAA) and evaluations of their anticancer, antioxidant, and molecular binding efficacy. The optical absorption peak at 380 nm from UV−visible (UV−vis) analysis revealed the formation of ZC NPs. X-ray diffraction (XRD) results revealed the fabrication of mixed-phase crystals with hexagonal and monoclinic structures of ZC NPs with an average crystallite size of 14 nm. Moreover, the biosynthesis of ZC NPs with a spherical morphology and an average particle size of 13.09 nm was confirmed by scanning electron microscopy (SEM), energy-dispersive X-ray (EDX), and transmission electron microscopy (TEM) results. Fourier transform infrared (FTIR) and thermogravimetric analysis (TGA/DTA) spectroscopy confirmed the involvement of functional groups from LEAA during the synthesis of ZC NPs. ZC NPs have exhibited the ferric ion reducing power (FRAP) with an absorbance of 1.826 ± 0.00 at 200 μg/ mL and DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) radical scavenging activity of 95.71 ± 0.02% at 200 μg/mL with an IC50 value of 3.28 μg/mL. Moreover, ZC NPs had shown a promising in vitro anticancer activity of 89.20 ± 0.038 at 500 μg/mL with an IC50 value of 33.12 μg/mL against breast cancer (MCF-7) cell lines. Likewise, ZC NPs have shown strong binding affinity (−8.50 kcal/mol) against estrogen receptor α (ERα) in molecular docking simulations. These findings suggested that the biosynthesized ZC NPs could be used as promising antioxidant and anticancer drug candidates, particularly for breast cancer ailments. However, the in vivo cytotoxicity test will be recommended to ensure further use of ZC NPs.

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Section: Introductionmentioning

confidence: 99%

Biosynthesis of Artemisia abyssinica Leaf Extract-Mediated Bimetallic ZnO–CuO Nanoparticles: Antioxidant, Anticancer, and Molecular Docking Studies

Orshiso,

Zereffa,

Murthy

et al. 2023

ACS Omega

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Investigating the Anticancer and Antioxidant Potentials of a Polymer-Grafted Sodium Alginate Composite Embedded with CuO and TiO2 Nanoparticles

Nemr,

Abdel-wahab,

Hamza

et al. 2024

J Polym Environ

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In this study, we conducted the synthesis of a composite material by grafting an acrylonitrile-co-styrene (AN-co-St) polymer into sodium alginate and incorporating CuO (copper oxide) and TiO2 (titanium dioxide) nanoparticles. The primary objective was to investigate the potential anticancer and antioxidant activities of the composite material. First, CuO and TiO2 nanoparticles were synthesized and characterized for their size, morphology, and surface properties. Subsequently, these nanoparticles were integrated into the sodium alginate matrix, which had been grafted with the AN-co-St polymer, resulting in the formation of the composite material. To confirm successful nanoparticle incorporation and assess the structural integrity of the composite, various techniques such as X-ray diffraction analysis (XRD), scanning electron microscopy-energy dispersive X-ray analysis (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA) were employed. The composite material’s anticancer and antioxidant activities were then evaluated. In vitro cell viability assays using the HepG-2 cell line were performed to assess potential cytotoxic effects, while antioxidant (DPPH) assays were conducted to determine the composite’s ability to scavenge free radicals and protect against oxidative stress. Preliminary results indicate that the composite material demonstrated promising anticancer and antioxidant activities. The presence of CuO and TiO2 nanoparticles within the composite contributed to these effects, as these nanoparticles are known to possess anticancer and antioxidant properties. Furthermore, the grafting of the AN-co-St polymer into sodium alginate enhanced the overall performance and stability of the composite material.

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One-Pot Biopreparation of Trimetallic ZnO–MgO–CuO Nanoparticles: Enhanced Cytotoxicity, Antibacterial Activities and Molecular Docking Studies

Orshiso,

Zereffa,

Murthy

et al. 2024

Chemistry Africa

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Nowadays, metal oxide nanoparticles (MO NPs) are powerful tools for biological applications due to their distinctive features. Moreover, the biological efficacy of multimetallic NPs is more fascinating because of their structural modifications and synergistic effects. This study utilized the one-pot green route to fabricate trimetallic ZnO-MgO-CuO (ZMC) NPs employing a greener reducing agent from Artemisiaabyssinica leaf extract (AALE). The crystal structure, size, compositions, shapes, and external topology of ZMC NPs were characterized by Fourier transform infrared (FTIR), UV–Visible (UV–vis), X-ray diffraction (XRD), scanning electron microscopy (SEM), energy-dispersive X-ray (EDX), and transmission electron microscopy combined with selected area electron diffraction (TEM/HRTEM-SAED). The outcomes suggested that the bio-prepared ZMC NPs are highly crystalline and have hexagonal structures lattice with monoclinic symmetry and spherical morphology with average crystalline and particle sizes of 14.67 and 15.13 nm, respectively. Using MTT assay, the bio-prepared ZMC NPs demonstrated high inhibition percentage (94.37 ± 0.14 at 250 mg/mL) with an IC50 value of 24.83 mg/mL for MCF-7 cell lines. The in-vitro antibacterial potential of ZMC NPs has been evaluated against four bacterial (Gram-positive and Gram-negative) strains and has demonstrated the highest inhibition zone (35 ± 0.03 mm) against the S. aureus strain and the lowest inhibition zone (31 ± 0.11) against the E. coli strain. Moreover, ZMC NPs have also shown strong molecular binding interactions with amino acids of estrogen receptor (ERα), S. aureus, and E. coli with binding energies of − 9.85, − 12.31, and − 6.04 kcal/mole, respectively. Graphical Abstract

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Synthesis and characterization of cerium, silver and copper oxide nanoparticles and their anticancer potential of hepatocellular carcinoma HepG2 cancer cells

Cited by 10 publications

References 52 publications

Biosynthesis of Artemisia abyssinica Leaf Extract-Mediated Bimetallic ZnO–CuO Nanoparticles: Antioxidant, Anticancer, and Molecular Docking Studies

Biosynthesis of Artemisia abyssinica Leaf Extract-Mediated Bimetallic ZnO–CuO Nanoparticles: Antioxidant, Anticancer, and Molecular Docking Studies

Investigating the Anticancer and Antioxidant Potentials of a Polymer-Grafted Sodium Alginate Composite Embedded with CuO and TiO2 Nanoparticles

One-Pot Biopreparation of Trimetallic ZnO–MgO–CuO Nanoparticles: Enhanced Cytotoxicity, Antibacterial Activities and Molecular Docking Studies

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