Synthesis and characterization of chitosan–multiwalled carbon nanotubes/hydroxyapatite nanocomposites for bone tissue engineering

Abstract

Chitosan-multiwalled carbon nanotubes/hydroxyapatite nanocomposites were synthesized by a novel in situ precipitation method. The electrostatic adsorption between multiwalled carbon nanotubes and chitosan was investigated and explained by Fourier transform infrared spectroscopy analysis. Morphology studies showed that uniform distribution of hydroxyapatite particles and multiwalled carbon nanotubes in the polymer matrix was observed. In chitosan-multiwalled carbon nanotubes/hydroxyapatite nanocomposites, the diameters of multiwalled carbon nanotubes were about 10 nm. The mechanical properties of the composites were evaluated by measuring their compressive strength and elastic modulus. The elastic modulus and compressive strength increased sharply from 509.9 to 1089.1 MPa and from 33.2 to 105.5 MPa with an increase of multiwalled carbon/chitosan weight ratios from 0 to 5 %, respectively. Finally, the cell biocompatibility of the composites was tested in vitro, which showed that they have good biocompatibility. These results suggest that the chitosan-multiwalled carbon nanotubes/hydroxyapatite nanocomposites are promising biomaterials for bone tissue engineering.

Albumin adsorption study onto hydroxyapatite-multiwall carbon nanotube based composites

“…When fMWCNT and chitosan is added to HAP the peak intensity decreased, which suggests a poorer crystallinity state [45,49]. These crystallographic structures of the CS-fMWCNT-HAP and CS-fMWCNT-HAPSi nanocomposites were closer to the natural bone mineral (biological apatite) in terms of the degree of crystallinity [50]. …”

In Situ Biomimetic Synthesis of Gelatin-Carbon Nanotube-Hydroxyapatite Biocomposites as Bone Filler

“…In the last 8 years, we have prepared different composites via in situ biomimetic methods using biopolymers such as . In this work, we designed new composites of gelatin-MWCNTs-HA by leveraging the suitable bioactivity and biocompatibility properties of gelatin-HA nanocomposites 2,5 , the excellent mechanical properties of CNTs 25 and the positive effects of functionalized CNTs on cell growth, biocompatibility, bio mineralization and bone formation 13,25 . The aim was using negative functional groups of MWCNTs and gelatin as active binding sites for in situ nucleation of HA in polymeric matrix template that leads to biocomposites formation.…”

“…The aim was using negative functional groups of MWCNTs and gelatin as active binding sites for in situ nucleation of HA in polymeric matrix template that leads to biocomposites formation. Ultrasonic method has been exploited the dispersion of MWCNTs in the gelatin matrix to conquer van der Waals interactions between CNTs and create positive charges on the carboxyl groups of MWCNTs that can act as electrostatic attraction sites for negative functional groups of gelatin 13 . As it is shown schematically in Fig.…”

“…2 shows the FT-IR spectra of MWCNT, gelatin, HA and composite samples (S-1, S-2 and S-3). In MWCNT, bands at 1120, 1720, can be attributed to the C-O , C=O stretching of carboxylic acids whereas bending and stretching vibration of hydroxyl group presented peaks at 672 and 3729 cm -1 , respectively [13]. Gelatin spectra showed the bands at 3438 cm "1 and 3423 cm "1 assigned to the OH and -NH stretching of secondary amide while peaks at 2922 and 1640 cm -1 corresponded to the C-H and the C=O stretching.…”

In vivo Study of the Osteoregenerative Potential of Polymer Membranes Consisting of Chitosan and Carbon Nanotubes

“…The interest in CNTs has been growing because of their ability to induce and conduct Hydroxyapatite is another widely used product because it permits the phenotypic expression of bone cells. In addition, hydroxyapatite accounts for up to 65% of the extracellular matrix composition of bone tissue [8][9][10] . In view of the beneficial properties of hydroxyapatite in bone regeneration, many studies have been conducted to obtain materials with characteristics that can increase the formation of hydroxyapatite in bone tissues 11 .…”