Synthesis and characterization of chitosan-polyacrylamide cryogels for the purification of human IgG by IMAC

Hamacek, Henrique Sepúlveda Del Rio; Bresolin, Igor Tadeu Lazzarotto; Fioravante, Igor Ferreira; Bueno, Sonia Maria Alves

doi:10.1016/j.procbio.2023.06.021

Process Biochemistry

2023

DOI: 10.1016/j.procbio.2023.06.021

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Synthesis and characterization of chitosan-polyacrylamide cryogels for the purification of human IgG by IMAC

Henrique Sepúlveda Del Rio Hamacek,

Igor Tadeu Lazzarotto Bresolin,

Igor Ferreira Fioravante

et al.

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2024

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Cited by 3 publications

References 48 publications

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Purification of pineapple bromelain by IMAC chromatography using chlorophyll-activated macroporous matrices

Porfirio,

Santos,

Alves

et al. 2024

Journal of Chromatography B

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Purification of pineapple bromelain by IMAC chromatography using chlorophyll-activated macroporous matrices

Porfirio,

Santos,

Alves

et al. 2024

Journal of Chromatography B

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Surface functionalized cryogels – characterization methods, recent progress in preparation and application

Behrendt,

Gottschaldt,

Schubert

2024

Mater. Horiz.

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Copper-Chelated Chitosan Microgels for the Selective Enrichment of Small Cationic Peptides

Jacquier,

Duffy,

O’Sullivan

et al. 2024

Gels

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Copper-chelated chitosan microgels were investigated as an immobilized metal affinity chromatography (IMAC) phase for peptide separation. The copper-crosslinked chitosan beads were shown to strongly interact with a range of amino acids, in a wide range of pH and saline conditions. The beads exhibited an affinity that seemed to depend on the isoelectric point of the amino acid, with the extent of uptake increasing with decreasing isoelectric point. This selective interaction with anionic amino acids resulted in a significant relative enrichment of the supernatant solution in cationic amino acids. The beads were then studied as a novel fractionation system for complex milk hydrolysates. The copper chitosan beads selectively removed larger peptides from the hydrolysate aqueous solution, yielding a solution relatively enriched in medium and smaller peptides, which was characterized both quantitatively and qualitatively by size exclusion chromatography (SEC). Liquid chromatography–mass spectrometry (LCMS) work provided comprehensive data on a peptide sequence level and showed that a depletion of the anionic peptides by the beads resulted in a relative enrichment of the cationic peptides in the supernatant solution. It could be concluded that after fractionation a dramatic relative enrichment in respect to small- and medium-sized cationic peptides in the solution, characteristics that have been linked to bioactivities, such as anti-microbial and cell-penetrating properties. The results demonstrate the use of the chitosan copper gel bead system in lab scale fractionation of complex hydrolysate mixtures, with the potential to enhance milk hydrolysate bioactivity.

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Synthesis and characterization of chitosan-polyacrylamide cryogels for the purification of human IgG by IMAC

Cited by 3 publications

References 48 publications

Purification of pineapple bromelain by IMAC chromatography using chlorophyll-activated macroporous matrices

Purification of pineapple bromelain by IMAC chromatography using chlorophyll-activated macroporous matrices

Surface functionalized cryogels – characterization methods, recent progress in preparation and application

Copper-Chelated Chitosan Microgels for the Selective Enrichment of Small Cationic Peptides

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