Synthesis and Characterization of Copper‐Nanoflowers with the Utilization of Medicinal Plant Extracts for Enhanced Various Enzyme Inhibitory Activities

Abstract

In this study, organic-inorganic hybrid nanoflowers were synthesized using methanolic extracts of the medicinal plants Ajuga chamaepitys subsp. chia var. chia, Achillea wilhelmsii, Bongardia chrysogonum, Malva sylvestris, Phlomis grandiflora var. grandiflora, Verbascum sp. together with copper ions (Cu 2 + ). The synthesized plant extract based-inorganic hybrid nanoflowers (PE-ihNFs) of A. chamaepitys subsp. chia var. chia (Ac-ihNFs), A. wilhelmsii (Aw-ihNfs), B. chrysogonum (Bc-ihNFs), M. sylvestris (Ms-ihNFs) P. grandiflora var. grandiflora (Pg-ihNFs), and Verbascum sp. (Vs-ihNFs) were characterized by Scanning Electron Microscopy (SEM), Energy-Dispersive X-ray (EDX), Fourier transform infrared spectrometry (FTIR), and X-Ray Diffraction (XRD). Also, several enzymes were selected to evaluate the enzyme inhibition activities of the synthesized PE-ihNFs. For the first-time, enzymes, tyrosinase, α-amylase and α-glucosidase, acetyl and butyryl cholinesterase inhibition activities of the PE-ihNFs with comparison to their plain plant extracts were evaluated in vitro. Results show that the among all the analyzed PE-ihNFs, demonstrated better α-glucosidase & α-amylase enzyme inhibition activity compared to the plain extracts. These initial studies are promising for the synthesis of these hybrid nanoflowers containing medicinal plant extracts, which might have commercial applications in the pharmaceutical and dermo-cosmetics industries.

Top Edible Wild Plants of Eastern Mediterranean Region. Part III: Antidiabetic Activity

“…But attempts to utilize this plant to treat DM are performed in indirect ways. U. Koca-Caliskan and her colleagues prepared copper nanoflowers using methanolic extract of M. sylvestris as a reductant [119]. These nanoflowers successfully inhibited α-amylase and α-glucosidase.…”

Influence of Metal Ions and Organic Solutions on Activity and Stability of Beta‐Glucosidase Nanoflowers

“…The fluorescent and visual bimodal sensing platform exhibited good sensitivity for beta‐glucosidase activity assay with a detection limit of 0.33 UL −1 under the optimum conditions. Koca‐Caliskan and coworkers have synthesized a plant extract based inorganic hybrid nanoflower, and for the first time, demonstrated superior α‐glucosidase and α‐amylase enzyme inhibition activity compared to plain extracts [20] . Gulmez synthesized β‐glucosidase hybrid nanoflower by using the fast sonication method to investigate glucose tolerance, anti‐protease activity, recyclability, and levels of total antioxidant and total oxidative capacity [21] .…”

“…Koca-Caliskan and coworkers have synthesized a plant extract based inorganic hybrid nanoflower, and for the first time, demonstrated superior α-glucosidase and α-amylase enzyme inhibition activity compared to plain extracts. [20] Gulmez synthesized β-glucosidase hybrid nanoflower by using the fast sonication method to investigate glucose tolerance, antiprotease activity, recyclability, and levels of total antioxidant and total oxidative capacity. [21] Sonochemical methods, with their advantages of faster reaction rates attributed to localized heating and enhanced mass transfer, contrast with conventional methods that often necessitate longer reaction times.…”

Solid‐State Synthesis of Liquorice‐Stabilized Copper‐Based Nanoparticles: Structural and Catalytic Studies

“…The aqueous extract of Mentha Longifolia leaf was used as a reducing/stabilizing agent in the green synthesis of Au nanoparticles [12] . Cu‐nanoflowers were prepared using some medicinal plant extracts [13] . Also, metallic nanocomposites such as Mn−Cu and Ni−Cu−Zn had been synthesized using Vinca rosea [14] and Centella Asiatica , [15] respectively.…”

“…[12] Cu-nanoflowers were prepared using some medicinal plant extracts. [13] Also, metallic nanocomposites such as MnÀ Cu and NiÀ CuÀ Zn had been synthesized using Vinca rosea [14] and Centella Asiatica, [15] respectively.…”