Synthesis and Characterization of Dual Stimuli Responsive Macromers Based on Poly(<i>N</i>-isopropylacrylamide) and Poly(vinylphosphonic acid)

Kretlow, James D.; Hacker, Michael C.; Klouda, Leda; Ma, Brandy B; Mikos, Antonios G.

doi:10.1021/bm9014182

Cited by 39 publications

(27 citation statements)

References 82 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The latter involves the functionalization of hydrogels with negatively charged side chains, or anionic phosphate, carboxyl, or hydroxyl groups that can bind calcium, mimicking the natural process in bone tissue [35]. Both vinylphosphonic acid and silanol groups have been copolymerized with PNiPAAm to create hydrogels exhibiting calcium-binding abilities and apatite formation in vitro [37, 38]. However, with these considerations in mind, the presence of anionic chemical groups is not dominantly affecting mineralization in this study since the carboxyl and hydroxyl presenting-TGM/DBA hydrogels presented less bone formation and mineralization than the TGM only hydrogels.…”

Section: Discussionmentioning

confidence: 99%

In vitro and in vivo evaluation of self-mineralization and biocompatibility of injectable, dual-gelling hydrogels for bone tissue engineering

Ekenseair

Spicer

et al. 2015

Journal of Controlled Release

Self Cite

View full text Add to dashboard Cite

In this study, we investigated the mineralization capacity and biocompatibility of injectable, dual-gelling hydrogels in a rat cranial defect as a function of hydrogel hydrophobicity from either the copolymerization of a hydrolyzable lactone ring or the hydrogel polymer content. The hydrogel system comprised a poly(N-isopropylacrylamide)-based thermogelling macromer (TGM) and a polyamidoamine crosslinker. The thermogelling macromer was copolymerized with (TGM/DBA) or without (TGM) a dimethyl-γ-butyrolactone acrylate (DBA)-containing lactone ring that modulated the lower critical solution temperature and thus, the hydrogel hydrophobicity, over time. Three hydrogel groups were examined: (1) 15 wt% TGM, (2) 15 wt% TGM/DBA, and (3) 20 wt% TGM/DBA. The hydrogels were implanted within an 8 mm critical size rat cranial defect for 4 and 12 weeks. Implants were harvested at each timepoint and analyzed for bone formation, hydrogel mineralization and tissue response using microcomputed tomography (microCT). Histology and fibrous capsule scoring showed a light inflammatory response at 4 weeks that was mitigated by 12 weeks for all groups. MicroCT scoring and bone volume quantification demonstrated similar bone formation at 4 weeks that was significantly increased for the more hydrophobic hydrogel formulations - 15 wt% TGM and 20 wt% TGM/DBA - from 4 weeks to 12 weeks. A complementary in vitro acellular mineralization study revealed that the hydrogels exhibited calcium binding properties in the presence of serum-containing media, which was modulated by the hydrogel hydrophobicity. The tailored mineralization capacity of these injectable, dual-gelling hydrogels with hydrolysis-dependent hydrophobicity presents an exciting property for their use in bone tissue engineering applications.

show abstract

Section: Discussionmentioning

confidence: 99%

In vitro and in vivo evaluation of self-mineralization and biocompatibility of injectable, dual-gelling hydrogels for bone tissue engineering

Ekenseair

Spicer

et al. 2015

Journal of Controlled Release

Self Cite

View full text Add to dashboard Cite

show abstract

“…Thermoresponsive polymers are a type of “smart” materials that respond to temperature and can be used in several biomedical applications such as tissue engineering, as wound dressings and in drug, radinuclide, and protein delivery . A special type of thermoresponsive polymers are ABA triblock copolymers.…”

Section: Introductionmentioning

confidence: 99%

Thermoresponsive gels based on ABA triblock copolymers: Does the asymmetry matter?

Ward

Georgiou

2013

J. Polym. Sci. Part A: Polym. Chem.

View full text Add to dashboard Cite

The aim of this study was to investigate the effect of the asymmetry of the triblock copolymers on their thermoresponsive self‐assembly behavior. To this end, nine ABA‐type triblock copolymers with n‐butyl methacrylate and 2‐(dimethylamino)ethyl methacrylate (DMAEMA) consisting of the A and the B blocks, respectively, were synthesized. Polymers of three different DMAEMA contents (50, 60, and 70 wt %) were synthesized while varying the length ratio of the two hydrophobic A blocks. Specifically, one symmetric ABA triblock copolymer and two asymmetric ABA′ triblock copolymers with the length of the second A block to be twice or four times bigger than the length of the first A block (AB2A and AB4A triblock copolymer) were synthesized for each DMAEMA composition. Three statistical copolymers were also synthesized for comparison. The thermoresponsive behavior of the copolymers was studied and it was found that the cloud point and rheological properties of the polymers were strongly affected by the architecture (statistical vs. block) and less strongly by the DMAEMA composition and the asymmetry of the polymers. Nevertheless, interestingly the asymmetry of the ABA triblock copolymers did influence the thermoresponsive behavior with the more symmetric polymers presenting a sol–gel transition at lower temperatures. © 2013 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2013, 51, 2850–2859.

show abstract

“…By using stimuli responsive polymers, NC hydrogels with enhanced mechanical, optical, and swelling properties as well as environmental‐susceptibility have been obtained . Poly( N ‐isopropylacrylamide) (PNIPA or PNIPAM) is the most commonly used stimuli‐responsive polymer for the preparation of responsive NC hydrogels . PNIPA shows a well‐defined coil‐to‐globule transition at its lower critical solution temperature (LCST 32 °C) because it contains both hydrophilic and hydrophobic groups in one monomer unit.…”

Section: Nanocomposite Hydrogels Of Nanomaterials As Biomaterialsmentioning

confidence: 99%

Self-assembled Monolayers and Nanocomposite Hydrogels of Functional Nanomaterials for Tissue Engineering Applications

2014

View full text Add to dashboard Cite

In biotechnology, SAMs and NC hydrogels of functional nanomaterials are of high interest as 2D and 3D cell culture systems, respectively, to mimic natural ECM and to control cell behaviors. Advanced biotechnological approaches often use nanoscale topography together with suitable surface functionalization, as a model of ECM, to control cell behaviors and tissue formation. SAMs of NMs are effective ECM models as 2D surfaces due to their larger nanostructured surface areas which provide higher molecular density by functionalization on a planar substrate than molecular SAMs. Additionally, NC hydrogels, produced by cross-linking of organic polymers with NMs, are excellent candidates as 3D cell culture systems owing to their optical, cell-compatibility and the extraordinary mechanical properties.

show abstract

Synthesis and Characterization of Dual Stimuli Responsive Macromers Based on Poly(N-isopropylacrylamide) and Poly(vinylphosphonic acid)

Cited by 39 publications

References 82 publications

In vitro and in vivo evaluation of self-mineralization and biocompatibility of injectable, dual-gelling hydrogels for bone tissue engineering

In vitro and in vivo evaluation of self-mineralization and biocompatibility of injectable, dual-gelling hydrogels for bone tissue engineering

Thermoresponsive gels based on ABA triblock copolymers: Does the asymmetry matter?

Self-assembled Monolayers and Nanocomposite Hydrogels of Functional Nanomaterials for Tissue Engineering Applications

Contact Info

Product

Resources

About