Synthesis and Characterization of Endophytic Fungi, Cladosporium perangustum Mediated Silver Nanoparticles and their Antioxidant, Anticancer and Nano-toxicological Study

Govindappa, M.; Lavanya, Madhavaraj; Aishwarya, P; Pai, K. S. R.; Lunked, Prathiksha; Hemashekhar, B.; Arpitha, BM; Ramachandra, Y. L.; Raghavendra, Vinay B.

doi:10.1007/s12668-020-00719-z

Cited by 43 publications

(23 citation statements)

References 26 publications

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“…The plausible mechanism of action of the nanoparticles is through mitochondrial apoptosis, DNA impairment and induced detention of cytokinesis (El-Batal et al, 2015). Silver nanoparticles synthesized from the water extract of the endophytic fungi, Cladosporium perangustum has been found to reduce the viability of the MCF-7 cells through enhancement in the levels of caspase-3, caspase-7, caspase-8, and caspase-9 expression (Govindappa et al, 2020). Biocompatible terbium oxide nanoparticles synthesized using the biomass of fungus Fusarium oxysporum were effective in dose-dependent cytotoxicity in MG-63 and Saos-2 cell-lines while being nontoxic to primary osteoblasts; ROS production was enhanced and apoptosis was confirmed with nanoparticle treatment (Iram et al, 2016).…”

Section: Anti-cancer Agentsmentioning

confidence: 99%

Microbial Nano-Factories: Synthesis and Biomedical Applications

et al. 2021

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In the recent times, nanomaterials have emerged in the field of biology, medicine, electronics, and agriculture due to their immense applications. Owing to their nanoscale sizes, they present large surface/volume ratio, characteristic structures, and similar dimensions to biomolecules resulting in unique properties for biomedical applications. The chemical and physical methods to synthesize nanoparticles have their own limitations which can be overcome using biological methods for the synthesis. Moreover, through the biogenic synthesis route, the usage of microorganisms has offered a reliable, sustainable, safe, and environmental friendly technique for nanosynthesis. Bacterial, algal, fungal, and yeast cells are known to transport metals from their environment and convert them to elemental nanoparticle forms which are either accumulated or secreted. Additionally, robust nanocarriers have also been developed using viruses. In order to prevent aggregation and promote stabilization of the nanoparticles, capping agents are often secreted during biosynthesis. Microbial nanoparticles find biomedical applications in rapid diagnostics, imaging, biopharmaceuticals, drug delivery systems, antimicrobials, biomaterials for tissue regeneration as well as biosensors. The major challenges in therapeutic applications of microbial nanoparticles include biocompatibility, bioavailability, stability, degradation in the gastro-intestinal tract, and immune response. Thus, the current review article is focused on the microbe-mediated synthesis of various nanoparticles, the different microbial strains explored for such synthesis along with their current and future biomedical applications.

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Section: Anti-cancer Agentsmentioning

confidence: 99%

Microbial Nano-Factories: Synthesis and Biomedical Applications

et al. 2021

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“…The findings significantly support the use of AgNPs as natural antioxidants for health protection against a wide range of oxidative stressors associated with degenerative diseases. This antioxidant validation is essential for synthesized AgNPs before their utilization against experimental models such as mice or against humans (Manjunath Hulikere & Joshi, 2019; Govindappa et al, 2020; Mistry et al, 2021; Sulaiman et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Biogenic synthesis of silver nanoparticles mediated by the consortium comprising the marine fungal filtrates of Penicillium oxalicum and Fusarium hainanense along with their antimicrobial, antioxidant, larvicidal and anticancer potency

Thakor

Mistry

Patel³

et al. 2022

Journal of Applied Microbiology

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Aim: To biosynthesize silver nanoparticles (AgNPs) using fungal isolates [DS-2 (Penicillium oxalicum) and DW-8 (Fusarium hainanense)] as well as their mixed cellfree filtrate (CFF) acting as a consortium (DSW-28) and their bio-potentials. Methods and Results:The fungi (DS-2 and DW-8) were harvested and CFF was prepared. CFF of each fungus and their mixture were reacted with silver nitrate solution under dark conditions for the synthesis of AgNPs. The UV-Visible spectra determined the surface plasmon resonance at 438, 441 and 437 nm for the AgNPs synthesized by DS-2, DW-8 and DSW-28, respectively. The band gap energy was found between 2.21 and 2.24 eV which depicted their ability to act as a semiconductor. The TEM imaging revealed the spherical shape and small size of AgNPs. The XRD pattern exhibited the crystalline structure corresponding to their peaks. The FTIR spectra indicate the presence of different functional groups present on the surface of AgNPs. The broad-spectrum antimicrobial activity was exhibited by AgNPs. The AgNPs also act as an effective antioxidant by depicting their radical scavenging activity against DPPH. Moreover, the AgNPs also inhibited the growth of fourth instar larvae of Aedes aegypti and Culex quinquefasciatus more efficiently in a dosedependent method. The biosynthesized AgNPs from DSW-28 showed a significant anticancer activity against MCF-7 cells. Conclusion:The silver nanoparticles synthesized by the CFF of two different fungi act synergistically in a consortium leading towards the production of silver nanoparticles with smaller size and higher bioactivity. Significance and Impact of the Study:The impressive bioactivity of the silver nanoparticles synthesized by the mixture of CFF of various fungi acting as a consortium recommends their prospective use in agriculture as well as in biomedical as an antimicrobial, antioxidant, larvicidal and anticancer agents in future.

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“…The DPPH test was used to assess the radical scavenging activity of AgNPs. Various concentrations (10,20,30,40,50,75, and 100 µg/mL) of 1 mL AgNPs were vortexed with 1 mL of freshly prepared 1 mM DPPH solution. The solution was then maintained in the dark for 30 min at room temperature.…”

Section: Antioxidant Activity By 22-diphenyl-1-picrylhydrazyl (Dpph) Methodsmentioning

confidence: 99%

“…The ndings signi cantly support the use of AgNPs as natural antioxidants for health protection against a wide range of oxidative stressors associated with degenerative diseases. This antioxidant validation is essential for synthesized AgNPs before their utilization against experimental models such as mice or against humans [4,24,29,30].…”

Section: Antioxidant Capacity Of Cff Derived Agnpsmentioning

confidence: 99%

Biogenic Synthesis of Silver Nanoparticles Mediated by the Consortium Comprising the Marine Fungal Filtrates of Penicillium Oxalicum and Fusarium Hainanense Along With Their Antimicrobial, Antioxidant and Larvicidal Potency

Thakor

Mistry

Patel

et al. 2021

Preprint

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The silver nanoparticles were biosynthesized using fungal isolates as well as their mixed cell free filtrate acting as a consortium namely, DS-2 (Penicillium oxalicum), DW-8 (Fusarium hainanense) and DSW-28, respectively. The UV-Visible spectra determined the surface plasmon resonance at 438, 441 and 437 nm for the silver nanoparticles synthesized by DS-2, DW-8 and DSW-28, respectively. The Tauc’s plot analysis disclosed the band gap energy between 2.21 eV to 2.24 eV which depicted their ability to act as a semiconductor. The TEM imaging revealed the spherical shape along with the average particle size of DS-2 as 11.14 ± 2.39 nm and DW-8 as 7.59 ± 1.31 nm whereas that of DSW-28 as 5.73 ± 0.4 nm. Thus, the silver nanoparticles synthesized by DSW-28 were smaller in size than the individual isolates. The XRD pattern of the silver nanoparticles exhibited the crystalline structure corresponding to their peaks. The FTIR spectra indicates the presence of different functional groups on the surface of the synthesized silver nanoparticles. The broad-spectrum antimicrobial activity was exhibited by silver nanoparticles synthesized by DSW-28 against Gram positive, Gram negative bacteria and plant pathogen Fusarium oxysporum than the individual fungal isolates. The DSW-28 synthesized silver nanoparticles also acts as an effective antioxidant by depicting their radical scavenging activity against DPPH. Moreover, the silver nanoparticles synthesized by DSW-28 also inhibited the growth of 4th instar larvae of Aedes aegypti and Culex quinquefasciatus more efficiently than DS-2 and DW-8 in a dose-dependent method. The impressive bioactivity of the silver nanoparticles synthesized by the mixture of cell free filtrate of various fungi acting as a consortium recommends their prospective use in agriculture as well as in biomedical as an antimicrobial, antioxidant and larvicidal agents in future.

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Synthesis and Characterization of Endophytic Fungi, Cladosporium perangustum Mediated Silver Nanoparticles and their Antioxidant, Anticancer and Nano-toxicological Study

Cited by 43 publications

References 26 publications

Microbial Nano-Factories: Synthesis and Biomedical Applications

Microbial Nano-Factories: Synthesis and Biomedical Applications

Biogenic synthesis of silver nanoparticles mediated by the consortium comprising the marine fungal filtrates of Penicillium oxalicum and Fusarium hainanense along with their antimicrobial, antioxidant, larvicidal and anticancer potency

Biogenic Synthesis of Silver Nanoparticles Mediated by the Consortium Comprising the Marine Fungal Filtrates of Penicillium Oxalicum and Fusarium Hainanense Along With Their Antimicrobial, Antioxidant and Larvicidal Potency

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