Synthesis and Characterization of Fumaric Acid Functionalized AgCl/Titania Nanocomposite with Enhanced Antibacterial Activity

Abstract

Silver based antimicrobial agents with TiO2 as a host support (AgCl/TiO2) are increasingly being evaluated for disinfection and therapeutic applications. In this work TiO2 supported silver chloride nanocomposites were synthesized and functionalized with fumaric acid. X-ray diffraction results showed that the TiO2 was in anatase phase and AgCI was in the cubic chalcoargyrite phase. The functionalization was confirmed by FTIR analysis. The fumaric acid functionalized AgCl/TiO2 (Fu-AgCl/TiO2) showed uniform particle size distribution in the range of 4-5 nm. The intensity size distribution of Fu-AgCl/TiO2 nanocomposite by DLS showed an average particle diameter of -290 nm with a polydispersity index (P. I.) of 0.47. Further, a high BET surface area of -320.7 m2/g was observed for Fu-AgCl/TiO2 with an average pore size distribution of 3.8 nm. The antimicrobial activity of Fu-AgCl/TiO2 and AgCl/TiO2 was evaluated by determining the MIC and MBC values, and it was observed that Fu-AgCl/TiO2 exhibited better antimicrobial activity as compared to the unfunctionalized nanocomposite. The antimicrobial activity of Fu-AgCl/TiO2 is mainly attributed to its superior physicochemical properties coupled with the synergistic action of both fumaric acid and silver ions. The changes in the cytoplasmic membrane of the cells due to the formation of intracellular ROS were observed by SEM imaging.

Collagen-based silver nanoparticles for biological applications: synthesis and characterization

“…This occurs due to amino group carries a positive charge and is present in AgNPcol [40,41] ( Figure 1D). The hydrodynamic diameter of the nanoparticles was between 64.34 nm and 81.76 nm and PDI value was between [42] showed PDI value of 0.47, which was very close to the result obtained from this study, although other PDI values were also reported [43][44][45]. The value of PDI is one of the major parameters used for selecting a low-polydispersity solution for the subsequent cell viability test.…”

Collagen-based silver nanoparticles for biological applications: synthesis and characterization

“…The hydrodynamic diameter of the nanoparticles was between 64.34 nm and 81.76 nm and PDI value was between 0.40 and 0.77. The AgNPs associated with titanium dioxide synthesized by Desai and Kowshik [ 42 ] showed PDI value of 0.47, which was very close to the result obtained from this study, although other PDI values were also reported [ 43 – 45 ]. The value of PDI is one of the major parameters used for selecting a low-polydispersity solution for the subsequent cell viability test.…”

Synthesis and characterization of TiO2 nanoparticles combined with geraniol and their synergistic antibacterial activity

“…These studies attributed the increase in antimicrobial susceptibility to modifications of TiO 2 with natural elements and non-metallic compounds [45,46,54,55]. Our MIC results are consistent with previous findings reporting MIC against various G-positive and G-negative bacteria in the similar range [45,[56][57][58][59] while other studies reported the MIC against MRSA to be much higher (7.0-13.0 mg/ml) [60,61]. Our study showed that the UV light on TiO 2 had no significant changes in the reduction rate.…”