Synthesis and characterization of gold nanoparticles and their toxicity in alternative methods to the use of mammals

Marcelino, M.; Borges, Felipe Azevedo; Scorzoni, Liliana; Singulani, Júnya de Lacorte; Garms, Bruna Cambraia; Niemeyer, Júlia Carina; Guerra, Nayrim Brizuela; Brasil, Giovana Sant’Ana Pegorin; Mussagy, Cassamo U.; Carvalho, Francisco Adriano de Oliveira; Scontri, Mateus; Mendes‐Giannini, Maria José Soares; Fusco‐Almeida, Ana Marisa; Herculano, Rondinelli Donizetti

doi:10.1016/j.jece.2021.106779

Cited by 17 publications

(5 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, SDS-AuNPs were reported to severely aggregate in culture media and various buffers, while their toxicity and cellular uptake are poorly characterized [12]. In good agreement with this study, the borohydride-citrate-capped AuNPs had a shelf lifetime of about 1 month, while citrate-capped AuNPs were stable for up to 6 months and had higher concentrations [26].…”

Section: Purification Of Core Gold Nanoparticles (Aunps)supporting

confidence: 85%

Experimental and Theoretical Screening of Core Gold Nanoparticles and Their Binding Mechanism to an Anticancer Drug, 2-Thiouracil

Lorenzana-Vázquez,

Adams,

Reyna

et al. 2023

Molecules

View full text Add to dashboard Cite

This study demonstrated the capability of two readily available optical spectroscopy tools, namely UV-Vis absorption spectrophotometry and Raman/surface-enhanced Raman spectroscopy, to select in a rapid and noninvasive manner the most homogenous gold nanoparticle (AuNP) models and to identify their chemical binding mechanism to 2-thiouracil (2-TU). 2-TU is an anticancer drug of great promise in the antiproliferative and photothermal therapies of cancer. The citrate-capped AuNPs emerged as the most stable as well as time- and cost-effective AuNP model out of the three widely used colloidal nanocores (citrate-, borohydride-citrate-, and sodium dodecyl sulfate (SDS)-capped AuNPs) that were examined. 2-TU chemically attached to the relatively monodispersed AuNPs via a chemisorption mechanism. The 2-TU-AuNPs complex formed through the covalent bonding of the S atom of 2-TU to the nanosurface in a vertical orientation. The spectroscopic results were then confirmed with the help of density functional theory (DFT) calculations and other physicochemical characterization tools for nanomaterials such as transmission electron microscopy (TEM), dynamic light scattering (DLS), and zeta potential. Overall, the purified 2-TU-AuNPs were found to be spherical, had an average diameter of 25 ± 2 nm, a narrow size distribution (1–30 nm), a sharp localized surface plasmon resonance (LSPR) peak at 525 nm, and a negative surface charge (−14 mV).

show abstract

Section: Purification Of Core Gold Nanoparticles (Aunps)supporting

confidence: 85%

Experimental and Theoretical Screening of Core Gold Nanoparticles and Their Binding Mechanism to an Anticancer Drug, 2-Thiouracil

Lorenzana-Vázquez,

Adams,

Reyna

et al. 2023

Molecules

View full text Add to dashboard Cite

show abstract

“…According to a study by Fayaz et al [69], the pharmacological properties of some antibiotics increase when combined with AgNPs. The possible mechanism of action of NP may be associated with van der Waals interactions and other weak bonds, leading to the interaction between AgNPs functionalized with antibiotics and the bacterial cell wall, resulting in the cell wall lysis and the NP penetration into the bacteria [70]. In addition to these benefits, the polymeric NRL matrix provides angiogenic activity, so, its use as a dressing is extremely effective in tissue regeneration.…”

Section: Hemolytic Activitymentioning

confidence: 99%

Silver nanoparticles effect on drug release of metronidazole in natural rubber latex dressing

et al. 2021

Self Cite

View full text Add to dashboard Cite

Natural rubber latex (NRL) from Hevea brasiliensis has shown great potential for dermal applications due to its angiogenesis capacity and biocompatibility. Metronidazole (MET) is a synthetic antibiotic used to treat various infections. However, this drug reports dangerous effects when high concentrations are administered. In this study, we used silver nanoparticles (AgNPs) in order to minimize these toxicological effects. For this, the membranes were characterized by physicochemical, molecular modeling, in vitro release and hemocompatibility assays. In the release assays, 14.25% of the MET and 27.28% of the AgNP + MET complex were released simultaneously, indicating that these nanoparticles work as drug carriers. Molecular modeling simulation helped to explain the in vitro release results, showing that AgNPs can interact more efficiently with MET molecules. Moreover, similarities in reactivity between NRL and MET suggested that some drug molecules may remain in the matrix during the release process. The membranes did not present significant hemolytic activity after 24 h of incubation. These results demonstrated that the NRL + AgNP + MET membrane can be used as a dressing in the treatment of infectious processes.

show abstract

“…Biological activities of αM and V, used as free compounds or their dendrimer conjugates, were also evaluated with a model nematode, Caenorhabditis elegans , a cost-effective and powerful model widely used for screening of bioactive compounds prior to be tested with higher animals [ 37 ]. C. elegans has been previously used in studies on the toxicity of drug-loaded nanoparticles, such as glibenclamide-loaded PVM/MA (poly(methylvinyl ether-co-maleic anhydride)) nanoparticles for oral delivery purposes [ 61 ], insulin-loaded PPA (PEG-polu(anhydride) conjugate) nanoparticles with the ability to mucus permeation for the oral administration of insulin [ 62 ], and the toxicity of bare nanoparticles, for example, zinc oxide nanoparticles (ZnO-NPs) [ 63 ] and gold nanoparticles (AuNPs) [ 64 ].…”

Section: Resultsmentioning

confidence: 99%

Synthesis and Properties of α-Mangostin and Vadimezan Conjugates with Glucoheptoamidated and Biotinylated 3rd Generation Poly(amidoamine) Dendrimer, and Conjugation Effect on Their Anticancer and Anti-Nematode Activities

2022

View full text Add to dashboard Cite

α-Mangostin and vadimezan are widely studied potential anticancer agents. Their biological activities may be improved by covalent bonding by amide or ester bonds with the third generation poly(amidoamine) (PAMAM) dendrimer, substituted with α-D-glucoheptono-1,4-lactone and biotin. Thus, conjugates of either ester- (G3gh4B5V) or amide-linked (G32B12gh5V) vadimezan, and equivalents of α-mangostin (G3gh2B5M and G32B12gh5M, respectively), were synthesized, characterized and tested in vitro against cancer cells: U-118 MG glioma, SCC-15 squamous carcinoma, and BJ normal human fibroblasts growth, as well as against C. elegans development. α-Mangostin cytotoxicity, stronger than that of Vadimezan, was increased (by 2.5–9-fold) by conjugation with the PAMAM dendrimer (with the amide-linking being slightly more effective), and the strongest effect was observed with SCC-15 cells. Similar enhancement of toxicity resulting from the drug conjugation was observed with C. elegans. Vadimezan (up to 200 µM), as well as both its dendrimer conjugates, was not toxic against both the studied cells and nematodes. It showed an antiproliferative effect against cancer cells at concentrations ≥100 µM. This effect was significantly enhanced after conjugation of the drug with the dendrimer via the amide, but not the ester bond, with G32B12gh5V inhibiting the proliferation of SCC-15 and U-118 MG cells at concentrations ≥4 and ≥12 μM, respectively, without a visible effect in normal BJ cells. Thus, the drug delivery system based on the PAMAM G3 dendrimer containing amide bonds, partially-blocked amino groups on the surface, larger particle diameter and higher zeta potential can be a useful tool to improve the biological properties of transported drug molecules.

show abstract

Synthesis and characterization of gold nanoparticles and their toxicity in alternative methods to the use of mammals

Cited by 17 publications

References 72 publications

Experimental and Theoretical Screening of Core Gold Nanoparticles and Their Binding Mechanism to an Anticancer Drug, 2-Thiouracil

Experimental and Theoretical Screening of Core Gold Nanoparticles and Their Binding Mechanism to an Anticancer Drug, 2-Thiouracil

Silver nanoparticles effect on drug release of metronidazole in natural rubber latex dressing

Synthesis and Properties of α-Mangostin and Vadimezan Conjugates with Glucoheptoamidated and Biotinylated 3rd Generation Poly(amidoamine) Dendrimer, and Conjugation Effect on Their Anticancer and Anti-Nematode Activities

Contact Info

Product

Resources

About