Synthesis and characterization of hapten-quantum dots bioconjugates: Application to development of a melamine fluorescentimmunoassay

Costa‐Fernández, José M.; Pereiro, Rosario; Sanz‐Medel, Alfredo

doi:10.1016/j.talanta.2013.01.027

Cited by 16 publications

(12 citation statements)

References 19 publications

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“…Denatured/precipitated proteins/macromolecules are naturally absorbents, and thus can adsorb small molecules like melamine, resulting in the loss of melamine in supernatant for the SERS detection. In spite of the relatively lower recovery, the maximum of melamine content of 2.5 ppm (20 μM) in milk regulated by many international agencies can be easily detected (Wu et al, 2012 ; Trapiella-Alfonso et al, 2013 ). These results have demonstrated that the wax/AuNPs-analyte/AuNPs based SERS sensing strategy holds great potential in real applications for food safety and environment protection.…”

Section: Resultsmentioning

confidence: 99%

A Simple SERS-Based Trace Sensing Platform Enabled by AuNPs-Analyte/AuNPs Double-Decker Structure on Wax-Coated Hydrophobic Surface

Luo

Huang

et al. 2018

Front. Chem.

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In this work, a simple and versatile SERS sensing platform enabled by AuNPs-analyte/AuNPs double-decker structure on wax-coated hydrophobic surface was developed using a portable Raman spectrometer. Wax-coated silicon wafer served as a hydrophobic surface to induce both aggregation and concentration of aqueous phase AuNPs mixed with analyte of interest. After drying, another layer of AuNPs was drop-cast onto the layer of AuNPs-analyte on the substrate to form double-decker structure, thus introducing more “hot spots” to further enhance the Raman signal. To validate the sensing platform, methyl parathion (pesticide), and melamine (a nitrogen-enrich compound illegally added to food products to increase their apparent protein content) were employed as two model compounds for trace sensing demonstration. The as-fabricated sensor showed high reproducibility and sensitivity toward both methyl parathion and melamine detection with the limit of detection at the nanomolar and sub-nanomolar concentration level, respectively. In addition, remarkable recoveries for methyl parathion spiked into lake water samples were obtained, while reasonably good recoveries for melamine spiked into milk samples were achieved. These results demonstrate that the as-developed SERS sensing platform holds great promise in detecting trace amount of hazardous chemicals for food safety and environment protection.

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Section: Resultsmentioning

confidence: 99%

A Simple SERS-Based Trace Sensing Platform Enabled by AuNPs-Analyte/AuNPs Double-Decker Structure on Wax-Coated Hydrophobic Surface

Luo

Huang

et al. 2018

Front. Chem.

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“…Sanz-Medel's group developed the melamine-BSA-CdSe/ZnS QDs (BSA = Bovine Serum Albumin) conjugates and demonstrated their use in melamine detection via complementary optical spectroscopy and molecular mass spectrometry procedures [47]. In this work, a competitive immunoassay tactic was engaged for detection of melamine, where the melamine and the immunoprobe [QDs:Mel-BSA:EDC (conjugation buffer) at 2:1:1500 ratio] compete for the binding sites of the immobilized antibody.…”

Section: Carbon and Quantum Dots For Melamine Detectionmentioning

confidence: 99%

Review on Nanomaterial-Based Melamine Detection

Shellaiah

Sun

2019

Chemosensors

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Illegal adulteration of milk products by melamine and its analogs has become a threat to the world. In 2008, the misuse of melamine with infant formula caused serious effects on babies of China. Thereafter, the government of China and the US Food and Drug Administration (FDA) limited the use of melamine of 1 mg/kg for infant formula and 2.5 mg/kg for other dairy products. Similarly, the World Health Organization (WHO) has also limited the daily intake of melamine of 0.2 mg/kg body weight per day. Many sensory schemes have been proposed by the scientists for carrying out screening on melamine poisoning. Among them, nanomaterial-based sensing techniques are very promising in terms of real-time applicability. These materials uncover and quantify the melamine by means of diverse mechanisms, such as fluorescence resonance energy transfer (FRET), aggregation, inner filter effect, surface-enhanced Raman scattering (SERS), and self-assembly, etc. Nanomaterials used for the melamine determination include carbon dots, quantum dots, nanocomposites, nanocrystals, nanoclusters, nanoparticles, nanorods, nanowires, and nanotubes. In this review, we summarize and comment on the melamine sensing abilities of these nanomaterials for their suitability and future research directions.

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“…Quantum dots (QDs) had been widely reported as fluorescent labels for biosensing developments and bio‐imaging . More recently, new emerging nanoparticles such as graphene quantum dots (GQDs) appears as promising fluorescent probes for the development of luminescent sensing assays .…”

Section: Introductionmentioning

confidence: 99%

“…Quantum dots (QDs) had been widely reported as fluorescent labels for biosensing developments and bio-imaging. [17][18][19] More recently, new emerging nanoparticles such as graphene quantum dots (GQDs) appears as promising fluorescent probes for the development of luminescent sensing assays. [19,20] GQDs are a kind of zerodimensional (0D) material that exhibit extraordinary optical and electronic properties due to their quantum confinement and edge effects.…”

Section: Introductionmentioning

confidence: 99%

Quenching of graphene quantum dots fluorescence by alkaline phosphatase activity in the presence of hydroquinone diphosphate

et al. 2018

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In this work, a turn-off photoluminescent sensing proof-of-concept based on blue luminescent graphene quantum dots (GQDs) as the fluorescent probe was developed. For that purpose, GQDs optical response was related with the catalytic enzymatic activity of alkaline phosphatase (ALP), in the presence of hydroquinone diphosphate (HQDP). The hydrolysis of HQDP by ALP generated hydroquinone (HQ). The oxidation of HQ, enzymatically produced, to p-benzoquinone (BQ) resulted in the quenching of GQDs fluorescence (FL). Therefore, the developed luminescent sensing mechanism allowed the FL quenching with ALP activity to be related and thus quantified the concentration of ALP down to 0.5 nM of enzyme. This innovative design principle appears as a promising tool for the development of enzymatic sensors based on ALP labeling with fluorescent detection or even for direct ALP luminescent quantification in an easy, fast and sensitive manner.

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Synthesis and characterization of hapten-quantum dots bioconjugates: Application to development of a melamine fluorescentimmunoassay

Cited by 16 publications

References 19 publications

A Simple SERS-Based Trace Sensing Platform Enabled by AuNPs-Analyte/AuNPs Double-Decker Structure on Wax-Coated Hydrophobic Surface

A Simple SERS-Based Trace Sensing Platform Enabled by AuNPs-Analyte/AuNPs Double-Decker Structure on Wax-Coated Hydrophobic Surface

Review on Nanomaterial-Based Melamine Detection

Quenching of graphene quantum dots fluorescence by alkaline phosphatase activity in the presence of hydroquinone diphosphate

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