Synthesis and characterization ofSe-adenosyl-L-selenohomocysteine selenoxide

Abstract

Selenium is an essential micronutrient in humans due to the important roles of the selenocysteine-containing selenoproteins. Organoselenium metabolites are generally found to be substrates for the biochemical pathways of their sulfur analogs, and the redox chemistry of selenomethionine and some other metabolites have been previously reported. We now report the first synthesis and characterization of Se-adenosylselenohomocysteine selenoxide (SeAHO) prepared via hydrogen peroxide oxidation of Se-adenosylselenohomocysteine (SeAH). The selenoxide SeAHO, in contrast to its corresponding sulfoxide S-adenosylhomocysteine (SAHO), can form hydrate, has an electrostatic interaction between the α-amino acid moiety and the highly polar selenoxide functional group, and readily oxidizes glutathione (GSH) and cysteine thiols.

MALDI TOF Mass Spectrometry Imaging of Blood Smear: Method Development and Evaluation

“…Dihydroneopterin phosphate accumulates in WBC as expected due to its role in WBC-mediated inflammatory response [ 21 ]. Selenoproteins and molybdate listed in Table 2 are endogenous compounds physiologically present in the blood cells, and their roll in cell metabolism is described in the literature [ 22 , 23 , 24 ]. The association of the rest of the metabolites ( Table 2 ) with different cell types is omitted since they are either redundant or there is a lack of evidence of their cell-specific role and expression.…”