Synthesis and characterization of indolicidin, a tryptophan‐rich antimicrobial peptide from bovine neutrophils *

Abel, Robert J. Van; Tang, Yueming; Rao, Vanga S.; Dobbs, Craig; Tran, Dat Q.; Bárány, George; Selsted, Michael E.

doi:10.1111/j.1399-3011.1995.tb01055.x

Cited by 48 publications

(3 citation statements)

References 23 publications

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“…The experiments listed above have also been used to study the antibacterial activity of natural and synthetic peptides. For example, the antibacterial activity of peptides such as LL-37, various defensins, indolicidin, and cecropins have all been studied using broth dilution, radial diffusion, hemolysis, NPN uptake, and ONPG influx assays. ,− …”

Section: Systems For Studying Membrane Permeabilizing Peptidesmentioning

confidence: 99%

Mechanistic Landscape of Membrane-Permeabilizing Peptides

et al. 2019

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Membrane permeabilizing peptides (MPPs) are as ubiquitous as the lipid bilayer membranes they act upon. Produced by all forms of life, most membrane permeabilizing peptides are used offensively or defensively against the membranes of other organisms. Just as nature has found many uses for them, translational scientists have worked for decades to design or optimize membrane permeabilizing peptides for applications in the laboratory and in the clinic ranging from antibacterial and antiviral therapy and prophylaxis to anticancer therapeutics and drug delivery. Here, we review the field of membrane permeabilizing peptides. We discuss the diversity of their sources and structures, the systems and methods used to measure their activities, and the behaviors that are observed. We discuss the fact that “mechanism” is not a discrete or a static entity for an MPP but rather the result of a heterogeneous and dynamic ensemble of structural states that vary in response to many different experimental conditions. This has led to an almost complete lack of discrete three-dimensional active structures among the thousands of known MPPs and a lack of useful or predictive sequence-structure–function relationship rules. Ultimately, we discuss how it may be more useful to think of membrane permeabilizing peptides mechanisms as broad regions of a mechanistic landscape rather than discrete molecular processes.

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Section: Systems For Studying Membrane Permeabilizing Peptidesmentioning

confidence: 99%

Mechanistic Landscape of Membrane-Permeabilizing Peptides

et al. 2019

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“…Because of Bill Wimley's work on leakage from vesicles induced by human defensins, Mike Selsted suggested that our two labs collaborate further on mechanisms of action of antimicrobial peptides. Importantly, his lab had sufficient financial resources to recruit two new postdocs to work on defensins and a novel 13AA peptide, indolicidin, that Mike had discovered (133). I had met Alex Ladokhin and Kalina Hristova at the 1994 Biophysical Society meeting in New Orleans.…”

Section: Fruits Of Collaborationmentioning

confidence: 99%

Fifty Years of Biophysics at the Membrane Frontier

White

2023

Annu. Rev. Biophys.

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The author first describes his childhood in the South and the ways in which it fostered the values he has espoused throughout his life, his development of a keen fascination with science, and the influences that supported his progress toward higher education. His experiences in ROTC as a student, followed by two years in the US Army during the Vietnam War, honed his leadership skills. The bulk of the autobiography is a chronological journey through his scientific career, beginning with arrival at the University of California, Irvine in 1972, with an emphasis on the postdoctoral students and colleagues who have contributed substantially to each phase of his lab's progress. White's fundamental findings played a key role in the development of membrane biophysics, helping establish it as fertile ground for research. A story gradually unfolds that reveals the deeply collaborative and painstakingly executed work necessary for a successful career in science. Expected final online publication date for the Annual Review of Biophysics, Volume 52 is May 2023. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

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“…Indolicidinbelongs to the family of cathelicidin AMPs. It was first discovered in bovine as tridecapeptide (13 amino acid long) amide from neutrophils showing bactericidal effect (Selsted et al, 1992) including its action on Staphylococcus aureus (ABEL et al, 1995). So far, seven protein coding cathelicidin gene have been identified which are traced to single cluster on chromosome 22 (Tomasinsig and Zanetti, 2005).…”

Section: Indolicidinmentioning

confidence: 99%

Antimicrobial Peptides in Farm animals: An updated review on its diversity, function, mode of action and therapeutic prospects

Kumar¹,

Ali²,

Singh³

et al. 2020

Preprint

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Antimicrobial peptides (AMPs) are the arsenals of the innate host defense system exhibiting the ancient evolutionarily conserved characteristics that is present in practically all forms of life. Recent years have witnessed emergence of antibiotic resistant bacteria compounded with a slow discovery rate for new antibiotics that has necessitated scientific efforts to search for alternatives to antibiotics. Research on the identification of AMPs has generated very encouraging evidences that they curb infectious pathologies and are also useful as novel biologics to function as immunotherapeutic agents. Being innate, they exhibit least toxicity to the host and exert wide spectrum of biological activity including low resistance among microbes, and increased wound healing actions. Notably, in veterinary science, the constant practice of massive doses of antibiotics with inappropriate withdrawal programs led to the high risk of livestock-associated antimicrobial resistance. Therefore, the world faces tremendous pressure for designing and devising strategies to mitigate the use of antibiotics in animals and keep it safe for the posterity. In this review, we illustrate the diversity of farm animals specific AMPs, their biochemical foundations, mode of action and prospective application in clinics. Subsequently, we present the data for their systematic classification by the major and minor groups, antipathogenic action, and allied bioactivities in the host. Finally, we address the limitations to their clinical implementation and envision areas for further advancement.

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Synthesis and characterization of indolicidin, a tryptophan‐rich antimicrobial peptide from bovine neutrophils *

Cited by 48 publications

References 23 publications

Mechanistic Landscape of Membrane-Permeabilizing Peptides

Mechanistic Landscape of Membrane-Permeabilizing Peptides

Fifty Years of Biophysics at the Membrane Frontier

Antimicrobial Peptides in Farm animals: An updated review on its diversity, function, mode of action and therapeutic prospects

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