Burns injuries induce a state of immunodepression that predisposes to a bacterial infectious complication that leads to several comorbid diseases and high mortality rate. Previous studies about anti-infl ammatory, antimicrobial and antioxidant properties of Aloe vera (L.) Burm., Calendula offi cinalis L. and Matricaria recutita L. are acknowledge by antimicrobial effects. Previous studies about anti-infl ammatory, antimicrobial and antioxidant properties of Aloe vera (L.) Burm., Calendula offi cinalis L. and Matricaria recutita L. are knowledge by antimicrobial effects. Bacterial cellulose membrane (nature BCM) is a potential carrier as a drug delivery system in the wound and burn treatment. The present study aimed to evaluate the antibacterial activity of extracts of A. vera, C. offi cinalis, and M. recutita incorporated in BCM against bacterial strains commonly present in wound and burns. The agar-dilution susceptibility testing was used to determine the minimum inhibitory concentration (MIC) for S. aureus, E. coli, and P. aeruginosa. The standardized extracts of A. vera, M. recutita, and C. offi cinalis were, respectively, used at 3.25% of total polysaccharides, 1% of apigenin 7-O-glucoside and 0.084% of total fl avonoids expressed in quercetin. The BCM incorporated with A. vera extract was effi cient to prevent the growth of P. aeruginosa and S. aureus. BCM loaded with C. offi cinalis inhibited the growth of S. aureus. The BCM loaded with A. vera and C. offi cinalis extract showed better antibacterial activities against P. aeruginosa and S. aureus and, consequently, properties to prevent infectious disease in the wound or burn caused by these bacteria.