Synthesis and characterization of nitrogen-functionalized graphene oxide in high-temperature and high-pressure ammonia

Baldovino, Fritzie Hannah B.; Quitain, A. T.; Dugos, Nathaniel P.; Roces, Susan A.; Koinuma, Masayoshi; Yuasa, Masayoshi; Kida, Tetsuya

doi:10.1039/c6ra22885b

Cited by 23 publications

(17 citation statements)

References 50 publications

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“…The

for the bare electrode was 0.6 while it increased to 0.7 for the aminated electrode suggesting the presence of more defect sites in the aminated rGO sample. These results are consistent with the results from Wei et al [ 27 ] and Baldovino et al [ 28 ]. Moreover, the full width at half maximum (FWHM) of the G peak of the Raman spectra is often used along with the

ratio and gives further information about the level of defects of the sample [ 29 ].…”

Section: Results and Discussionsupporting

confidence: 94%

“…Moreover, the full width at half maximum (FWHM) of the G peak of the Raman spectra is often used along with the

ratio and gives further information about the level of defects of the sample [ 29 ]. The FWHM of the G peak increased from 60.5 to 78.2 after the amination of rGO samples, which confirms the presence of N atoms on the surface [ 28 ].…”

Section: Results and Discussionmentioning

confidence: 83%

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A Label-Free DNA-Immunosensor Based on Aminated rGO Electrode for the Quantification of DNA Methylation

et al. 2021

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In this work, we developed a sandwich DNA-immunosensor for quantification of the methylated tumour suppressor gene O-6-methylguanine-DNA methyltransferase (MGMT), which is a potential biomarker for brain tumours and breast cancer. The biosensor is based on aminated reduced graphene oxide electrode, which is achieved by ammonium hydroxide chemisorption and anti-5-methylcytosine (anti-5mC) as a methylation bioreceptor. The target single-strand (ss) MGMT oligonucleotide is first recognised by its hybridisation with complementary DNA to form double-stranded (ds) MGMT, which is then captured by anti-5mC on the electrode surface due to the presence of methylation. Raman spectroscopy, X-ray photoelectron spectroscopy (XPS) and Scanning electron microscopy (SEM) techniques were used to characterise the electrode surface. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques were used for electrochemical measurements. Under optimised conditions, the proposed biosensor is able to quantify a linear range of concentrations of the MGMT gene from 50 fM to 100 pM with a limit of detection (LOD) of 12 fM. The sandwich design facilitates the simultaneous recognition and quantification of DNA methylation, and the amination significantly improves the sensitivity of the biosensor. This biosensor is label-, bisulfite- and PCR-free and has a simple design for cost-efficient production. It can also be tailor-made to detect other methylated genes, which makes it a promising detection platform for DNA methylation-related disease diagnosis and prognosis.

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“…The

ratio and gives further information about the level of defects of the sample [ 29 ].…”

Section: Results and Discussionsupporting

confidence: 94%

“…Moreover, the full width at half maximum (FWHM) of the G peak of the Raman spectra is often used along with the

Section: Results and Discussionmentioning

confidence: 83%

A Label-Free DNA-Immunosensor Based on Aminated rGO Electrode for the Quantification of DNA Methylation

et al. 2021

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“…Nitrogen-doped reduced graphene oxide is reported to contain three most desired, nitrogen bonding configurations namely pyrrolic-N, pyridinic-N, and graphitic N with different components of N 1s at 399.8–401.2 eV, 398.1–99.3 eV and 401.1–402.7 eV, respectively [76,77]. However, these N 1s positions vary in comparatively wide ranges in different studies.…”

Section: Resultsmentioning

confidence: 99%

Effect of Doping Temperatures and Nitrogen Precursors on the Physicochemical, Optical, and Electrical Conductivity Properties of Nitrogen-Doped Reduced Graphene Oxide

2019

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The greatest challenge in graphene-based material synthesis is achieving large surface area of high conductivity. Thus, tuning physico-electrochemical properties of these materials is of paramount importance. An even greater problem is to obtain a desired dopant configuration which allows control over device sensitivity and enhanced reproducibility. In this work, substitutional doping of graphene oxide (GO) with nitrogen atoms to induce lattice–structural modification of GO resulted in nitrogen-doped reduced graphene oxide (N-rGO). The effect of doping temperatures and various nitrogen precursors on the physicochemical, optical, and conductivity properties of N-rGO is hereby reported. This was achieved by thermal treating GO with different nitrogen precursors at various doping temperatures. The lowest doping temperature (600 °C) resulted in less thermally stable N-rGO, yet with higher porosity, while the highest doping temperature (800 °C) produced the opposite results. The choice of nitrogen precursors had a significant impact on the atomic percentage of nitrogen in N-rGO. Nitrogen-rich precursor, 4-nitro-ο-phenylenediamine, provided N-rGO with favorable physicochemical properties (larger surface area of 154.02 m2 g−1) with an enhanced electrical conductivity (0.133 S cm−1) property, making it more useful in energy storage devices. Thus, by adjusting the doping temperatures and nitrogen precursors, one can tailor various properties of N-rGO.

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“…The thermal behavior of GO has been consistent with the previous reports. 34,48 The decomposition temperature of oxygen-containing groups on TGA was shied to a high temperature due to the conversion of GO into CGO (Fig. 3b).…”

Section: Synthesis and Characterization Of Go Cgo Gobcmentioning

confidence: 99%

“…The enhancement of the thermal stability of GOBC was due to the successful attachment of large functional groups over the CGO surface. 42,48 The surface morphology of cross-linkers plays an important role in their functionalities while interacting with the polymer chains. The surface morphologies of GO, CGO, and GOBC were observed by FESEM and the microscopic images are presented in Fig.…”

Section: Synthesis and Characterization Of Go Cgo Gobcmentioning

confidence: 99%

Mechanically tough and highly stretchable poly(acrylic acid) hydrogel cross-linked by 2D graphene oxide

et al. 2020

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Synthesis and characterization of nitrogen-functionalized graphene oxide in high-temperature and high-pressure ammonia

Abstract: Successful N-functionalization of graphene oxide with high-temperature and high-pressure ammonia obtaining over 10% N-doping level.

Cited by 23 publications

References 50 publications

A Label-Free DNA-Immunosensor Based on Aminated rGO Electrode for the Quantification of DNA Methylation

A Label-Free DNA-Immunosensor Based on Aminated rGO Electrode for the Quantification of DNA Methylation

Effect of Doping Temperatures and Nitrogen Precursors on the Physicochemical, Optical, and Electrical Conductivity Properties of Nitrogen-Doped Reduced Graphene Oxide

Mechanically tough and highly stretchable poly(acrylic acid) hydrogel cross-linked by 2D graphene oxide

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