Synthesis and Characterization of Ramipril Embedded Nanospheres of Biodegradable Poly-&lt;I&gt;D&lt;/I&gt;,&lt;I&gt;L&lt;/I&gt;-Lactide-co-Glycolide and Their Kinetic Release Study

Basu, Tanushree; Pal, Bonamali; Singh, Satnam

doi:10.1166/asem.2016.1878

Cited by 8 publications

(4 citation statements)

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“…These observations are consistent with the reported literature, with peaks at 1550 cm −1 and 1630 cm −1 [23,37]. The poly (D, L-lactide-co-glycolide) grade 75:25 (PLGA) polymer (red) [24] showed, as expected, small but visible peaks at 2954 cm −1 , 2833 cm −1 , 1758 cm −1 , and 1093 cm −1 . Interestingly, the Zika MPs spectra (blue) displayed peaks similar to the PLGA polymer at 3348 cm −1 (OH bond), 2930 cm −1 (CH), 2855 cm −1 , 1745 cm −1 (carbonyl bond), 1085 cm −1 (CO bond), and 1550 cm −1 .…”

Section: Discussionsupporting

confidence: 92%

“…The intranasal (IN) route of vaccine delivery has gained increased popularity over the years. Vaccine delivery via the nasal cavity [ 24 ] is beneficial since the mucosa has an abundance of lymphatic tissue or nasal-associated lymphoid tissue (NALT) and specialized cells called microfold (M) cells ( Figure 2 ). This route is attractive because it induces a systemic and local mucosal immune response [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

“…These observations are consistent with the reported literature [ 23 ]. Small, but visible peaks at 2954 cm −1 , 2833 cm −1 , 1758 cm −1 , and 1093 cm −1 are representative of the poly (D, L-lactide-co-glycolide) grade 75:25 (PLGA) polymer [ 24 ]. In the Zika microparticle spectra (blue), the main peaks observed at 2930 cm −1 , 2855 cm −1 , 1745 cm −1 , 1085 cm −1 , 1550 cm −1 ).…”

Section: Figurementioning

confidence: 99%

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Intranasal Immunization for Zika in a Pre-Clinical Model

Shah,

Patel,

Bagwe

et al. 2024

Viruses

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Humans continue to be at risk from the Zika virus. Although there have been significant research advancements regarding Zika, the absence of a vaccine or approved treatment poses further challenges for healthcare providers. In this study, we developed a microparticulate Zika vaccine using an inactivated whole Zika virus as the antigen that can be administered pain-free via intranasal (IN) immunization. These microparticles (MP) were formulated using a double emulsion method developed by our lab. We explored a prime dose and two-booster-dose vaccination strategy using MPL-A® and Alhydrogel® as adjuvants to further stimulate the immune response. MPL-A® induces a Th1-mediated immune response and Alhydrogel® (alum) induces a Th2-mediated immune response. There was a high recovery yield of MPs, less than 5 µm in size, and particle charge of −19.42 ± 0.66 mV. IN immunization of Zika MP vaccine and the adjuvanted Zika MP vaccine showed a robust humoral response as indicated by several antibodies (IgA, IgM, and IgG) and several IgG subtypes (IgG1, IgG2a, and IgG3). Vaccine MP elicited a balance Th1- and Th2-mediated immune response. Immune organs, such as the spleen and lymph nodes, exhibited a significant increase in CD4+ helper and CD8+ cytotoxic T-cell cellular response in both vaccine groups. Zika MP vaccine and adjuvanted Zika MP vaccine displayed a robust memory response (CD27 and CD45R) in the spleen and lymph nodes. Adjuvanted vaccine-induced higher Zika-specific intracellular cytokines than the unadjuvanted vaccine. Our results suggest that more than one dose or multiple doses may be necessary to achieve necessary immunological responses. Compared to unvaccinated mice, the Zika vaccine MP and adjuvanted MP vaccine when administered via intranasal route demonstrated robust humoral, cellular, and memory responses. In this pre-clinical study, we established a pain-free microparticulate Zika vaccine that produced a significant immune response when administered intranasally.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

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Intranasal Immunization for Zika in a Pre-Clinical Model

Shah,

Patel,

Bagwe

et al. 2024

Viruses

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“…Na região de 220-270 nm também, pode haver contribuição do ligação -C=O do PLGA(50:50), onde, neste caso, o maior comprimento de onda está relacionado a nanopartículas(110,111), a região de entre 400-500 nm, também pode sugerir interação do PLGA(50:50) com o ativo (112). …”

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