Synthesis and Characterization of Serendipitous Dioxovanadates and Their DNA/BSA Interaction Studies and In Vitro Cytotoxic activity

Kothandan, Saraswathi; Sheela, Angappan

doi:10.1007/s10904-020-01815-z

Cited by 5 publications

(2 citation statements)

References 62 publications

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“…The number of binding sites n and binding constant K b were also calculated by employing the modified Stern–Volmer equation, Scatchard equation , (eq ) The emission spectra for ctDNA were recorded in the wavelength range of 300–500 nm by fixing the excitation wavelength at 290 nm in the absence and presence of 3i . The emission spectra of DNA were quenched by the successive addition of varying concentrations of 3i .…”

Section: Methodsmentioning

confidence: 99%

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Visible-Light-Prompted Synthesis and Binding Studies of 5,6-Dihydroimidazo[2,1-b]thiazoles with BSA and DNA Using Biophysical and Computational Methods

et al. 2022

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Fused heterocyclic systems containing a bridgehead nitrogen atom have emerged as imperative pharmacophores in the design and development of new drugs. Among these heterocyclic moieties, the imidazothiazole scaffold has long been used in medicinal chemistry for the treatment of various diseases. In this study, we have established a simplistic and environmentally safe regioselective protocol for the synthesis of 5,6-dihydroimidazo[2,1-b]thiazole derivatives from easily available reactants. The reaction proceeds through in situ formation of the α-bromodiketones ensuing trap with imidazolidine-2-thione to provide these versatile bicyclic heterocycles in excellent yields. The synthesized compounds were screened through the molecular docking approach for the most stable complex formation with bovine serum albumin (BSA) and calf thymus deoxyribonucleic acid (ctDNA). The selected compound was further studied using ex vivo binding studies, which revealed moderate interactions with BSA and ctDNA. The binding studies were performed using biophysical approaches including UV–visible spectroscopy, steady-state fluorescence, circular dichroism (CD), and viscosity parameters.

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Section: Methodsmentioning

confidence: 99%

“…The number of binding sites n and binding constant K b were also calculated by employing the modified Stern−Volmer equation, Scatchard equation 54,55 (eq 4)…”

Section: ■ Experimental Sectionmentioning

confidence: 99%