In this study some new compounds have been prepared including thereaction of diazoium salt of 1,4-phenylenediamine with 2-mercaptobenzaldehyde at (0-5)0C[F1]. Then Schiff bases [F2-F6] werethen synthesized through the reaction of compound [F1] withsubstituteds aniline in absolute ethanol, and Schiff bases were convertedinto 2,3 - dihyroquinazolin - 4(1H) - one derivatives [F7-F11] by thereaction with 2-amino benzoic acid in ethanol. The prepared compoundswere characterized by color and melting point, FT-IR, UV-Vis spectralanalysis. Some of the prepared compounds were identified by 1H-NMRspectra and C.H.N analysis. The antibacterial activities were studiedagainst different kinds of bacteria, in addition, the stability of prepareddyes which proved to be of high stability and bright colors was studies,in addition the conformation of prepared compounds at the lower energylevel were studied.