Synthesis and characterization of SPIO-loaded PEG-b-PS micelles as contrast agent for long-term nanoparticle-based MRI phantom

Abstract

Nanoparticle-based magnetic resonance imaging (MRI) phantom was developed from the suspension of magnetic nanoparticles composing methoxy poly(ethylene glycol)-block-poly(styrene) (PEG-b-PS) micelles and superparamagnetic iron oxide (SPIO) nanoparticles in the core of micelles. The size of SPIO-loaded micelles was determined by dynamic light scattering (DLS) and transmission electron microscopy. Larger-size micelles were found when SPIO loading was increased. The effect of the hydrophobic section of block copolymer on the size of micelles was studied by DLS. Transverse relaxivity was evaluated on both 1.5 and 3 T clinical MRI scanner. Higher SPIO-loading provided higher relaxivity, where the relaxivity of 10% SPIO-loaded PEG(5 kDa)-b-PS(5 kDa) were 144.0 and 174.0 m M −1 s −1 for 1.5 and 3 T MRI, respectively. This formulation showed stability over a 10-week period, and the standard deviations of the relaxivities were 3.0 and 8.0% for 1.5 and 3 T MRI, respectively. Thus, SPIO-loaded PEG-b-PS micelles have a potential to be applied as a contrast agent for nanoparticle-based MRI phantom.

Crosslinked Coating Improves the Signal‐to‐Noise Ratio of Iron Oxide Nanoparticles in Magnetic Particle Imaging (MPI)

“…The hydrodynamic diameter determined by dynamic light scattering (DLS) was around 180 nm at 25 °C, suggesting aggregation . However, higher diameters of SPIOs coated with PF127 than expected were reported previously .…”

“…After phase transfer using PF127DA, conjugation of biocompatible thiol functionalized poly(glycidol)s (PG−SH) to the PF127DA coating on the SPIO surface was achieved by thiol‐acrylate Michael addition, and coating identity was evaluated by Fourier transform infrared spectroscopy (Figure S4).…”

In Vitro Tracking of Sporozoites via Fluorescence Imaging and MRI Using Multifunctional Micelles

“…To enhance the visualization of internal organs, contrasting agents, such as superparamagnetic iron oxide (SPIO) nanoparticles, holmium, gadolinium­(III)-based contrasting agents, and manganese-based contrasting agents, can be used in conjunction with MRI. , These agents work by reducing the relaxation of surrounding water molecules . SPIO nanoparticles, known for their narrow size range of 3–15 nm, are widely utilized in various medical disciplines including imaging, in vitro bioseparation, magnetic hyperthermia, and tissue engineering. , Furthermore, the incorporation of SPIO nanoparticles in drug delivery systems enables accurate diagnosis through MRI utilization. In a study conducted by Mazumder et al, SPIO/doxorubicin-loaded glucose micelles were used to achieve precise cancer diagnosis in MRI images .…”

“…10 SPIO nano-particles, known for their narrow size range of 3−15 nm, are widely utilized in various medical disciplines including imaging, in vitro bioseparation, magnetic hyperthermia, and tissue engineering. 11,12 Furthermore, the incorporation of SPIO nanoparticles in drug delivery systems enables accurate diagnosis through MRI utilization. In a study conducted by Mazumder et al, SPIO/doxorubicin-loaded glucose micelles were used to achieve precise cancer diagnosis in MRI images.…”

Vancomycin conjugated iron oxide nanoparticles for magnetic targeting and efficient capture of Gram-positive and Gram-negative bacteria

“…8 Iron oxide nanoparticles (IONPs) have attracted an extensive attention because of their potential applications in biomedical eld for magnetic resonance imaging (MRI), magnetic hyperthermia, cancer therapy, drug delivery and biomolecule separation. [9][10][11][12][13] However, bare iron oxide nanoparticles have limitations that include colloidal instability, leaching out, toxicity, and degradation in biological medium. 14 Therefore, different efforts have been devoted for coating and surface modication of IONPs with different materials such as polymers, silica, and ligand molecules, dextran, and surfactants.…”