Synthesis and Characterization of <sup>18</sup>F-Interleukin-8 Using a Cell-Free Translation System and 4-<sup>18</sup>F-Fluoro-l-Proline

Harada, Ryuichi; Furumoto, Shozo; Yoshikawa, Takeo; Ishikawa, Yoichi; Shibuya, Kazuhiko; Okamura, Nobuyuki; Ishiwata, Kiichi; Iwata, Ren; Yanai, Kazuhiko

doi:10.2967/jnumed.115.162602

Cited by 10 publications

(4 citation statements)

References 37 publications

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“…Due to the relaxed amino acid specificity of aaRS, a few ncAAs that are structurally similar to canonical amino acids can be charged to native tRNAs by corresponding aaRS. Examples include selenomethionine ( Kigawa et al, 2002 ), fluorinated tryptophan analogs ( Mat et al, 2010 ), chlorinated tyrosine analogs ( Singh-Blom et al, 2014 ), 4- 18 F-fluoro- L -proline and 3,4-dihydroxy-l-phenylalanine ( Harada et al, 2016 ). Thus, structurally similar ncAAs can be incorporated in place of the corresponding native amino acids.…”

Section: Strategies For Eliminating Competitionmentioning

confidence: 99%

Emerging Methods for Efficient and Extensive Incorporation of Non-canonical Amino Acids Using Cell-Free Systems

Wang

Qiao

et al. 2020

Front. Bioeng. Biotechnol.

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Cell-free protein synthesis (CFPS) has emerged as a novel protein expression platform. Especially the incorporation of non-canonical amino acids (ncAAs) has led to the development of numerous flexible methods for efficient and extensive expression of artificial proteins. Approaches were developed to eliminate the endogenous competition for ncAAs and engineer translation factors, which significantly enhanced the incorporation efficiency. Furthermore, in vitro aminoacylation methods can be conveniently combined with cell-free systems, extensively expanding the available ncAAs with novel and unique moieties. In this review, we summarize the recent progresses on the efficient and extensive incorporation of ncAAs by different strategies based on the elimination of competition by endogenous factors, translation factors engineering and extensive incorporation of novel ncAAs coupled with in vitro aminoacylation methods in CFPS. We also aim to offer new ideas to researchers working on ncAA incorporation techniques in CFPS and applications in various emerging fields.

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Section: Strategies For Eliminating Competitionmentioning

confidence: 99%

Emerging Methods for Efficient and Extensive Incorporation of Non-canonical Amino Acids Using Cell-Free Systems

Wang

Qiao

et al. 2020

Front. Bioeng. Biotechnol.

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“…Due to the relaxed amino acid specificity of aaRS, a few ncAAs that are structurally similar to canonical amino acids can be charged to native tRNAs by corresponding aaRS. Examples include selenomethionine [71], fluorinated tryptophan analogs [72], chlorinated tyrosine analogs [73], 4-18F-fluoro-L-proline and 3,4-dihydroxy-l-phenylalanine [74]. Thus, structurally similar ncAAs can be incorporated in place of the corresponding native amino acids.…”

Section: Amino Acid Replacementmentioning

confidence: 99%

Emerging Methods for Efficient and Extensive Incorporation of Non Canonical Amino Acids using Cell-free Systems

Wu¹,

Wang²,

Qiao³

et al. 2020

Prime Archives in Biotechnology

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“…Introduction of ncAAs has also made its mark in the synthesis of radiolabeled proteins for PET imaging. In vitro translation in the presence of 18 F-4-fluoro-proline led to radiolabeled interleukin-8, which was employed by Yanai to detect tumors bearing IL8-receptors in mouse xenografts [28]. The inclusion of ncAAs can also contribute to the understanding of protein mechanisms and interactions.…”

Section: In Vitro Synthesis Of Modified Proteinsmentioning

confidence: 99%

In vitro genetic code reprogramming and expansion to study protein function and discover macrocyclic peptide ligands

Richardson

Dods

Abrigo

et al. 2018

Current Opinion in Chemical Biology

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The ability to introduce non-canonical amino acids into peptides and proteins is facilitated by working within in vitro translation systems. Non-canonical amino acids can be introduced into these systems using sense codon reprogramming, stop codon suppression, and by breaking codon degeneracy. Here, we review how these techniques have been used to create proteins with novel properties and how they facilitate sophisticated studies of protein function. We also discuss how researchers are using in vitro translation experiments with non-canonical amino acids to explore the tolerance of the translation apparatus to artificial building blocks. Finally, we give several examples of how non-canonical amino acids can be combined with mRNA-displayed peptide libraries for the creation of protease-stable, macrocyclic peptide libraries for ligand discovery.

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Synthesis and Characterization of ¹⁸F-Interleukin-8 Using a Cell-Free Translation System and 4-¹⁸F-Fluoro-l-Proline

Cited by 10 publications

References 37 publications

Emerging Methods for Efficient and Extensive Incorporation of Non-canonical Amino Acids Using Cell-Free Systems

Emerging Methods for Efficient and Extensive Incorporation of Non-canonical Amino Acids Using Cell-Free Systems

Emerging Methods for Efficient and Extensive Incorporation of Non Canonical Amino Acids using Cell-free Systems

In vitro genetic code reprogramming and expansion to study protein function and discover macrocyclic peptide ligands

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Synthesis and Characterization of 18F-Interleukin-8 Using a Cell-Free Translation System and 4-18F-Fluoro-l-Proline

Cited by 10 publications

References 37 publications

Emerging Methods for Efficient and Extensive Incorporation of Non-canonical Amino Acids Using Cell-Free Systems

Emerging Methods for Efficient and Extensive Incorporation of Non-canonical Amino Acids Using Cell-Free Systems

Emerging Methods for Efficient and Extensive Incorporation of Non Canonical Amino Acids using Cell-free Systems

In vitro genetic code reprogramming and expansion to study protein function and discover macrocyclic peptide ligands

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Synthesis and Characterization of ¹⁸F-Interleukin-8 Using a Cell-Free Translation System and 4-¹⁸F-Fluoro-l-Proline