Synthesis and characterization of α-1,3-alt-α-1,4-glucan (nigeran) ester derivatives

Togo, Azusa; Uechi, Keiko; Mizutani, Osamu; Kimura, Satoshi; Iwata, Tadahisa

doi:10.1016/j.polymer.2020.123343

Cited by 4 publications

(1 citation statement)

References 32 publications

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“…Therefore, they have little thermoplasticity and cannot be molded by heat-melting methods such as melt-spinning, but they can be useful thermoplastics by esterification. For example, paramylon esters can be melt-spun, dextran esters can be used as hot-melt adhesives, and nigeran (α-1,3- alt -α-1,4-glucan) esters can be used as melt-press films . However, the biodegradability of polysaccharide ester derivatives decreases as the degree of substitution increases. , This decrease in biodegradability is undesirable because it exacerbates environmental pollution from microplastics.…”

Section: Introductionmentioning

confidence: 99%

High Tensile Strength Regenerated α-1,3-Glucan Fiber and Crystal Transition

et al. 2021

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α-1,3-Glucan is a linear and crystalline polysaccharide which is synthesized by in vitro enzymatic polymerization from sucrose. A previous study reported that regenerated fibers of α-1,3glucan were prepared using a wet-spinning method. However, the mechanical properties were poorer than cellulose regenerated fibers. Then, in this study, the mechanical properties of the regenerated α-1,3-glucan fiber were improved by the transformation of the crystal structure and stretching. The regenerated fiber stretched in water and dehydrated by heating showed high tensile strength (18 cN/tex) that is comparable with that of viscose rayon. Moreover, the crystal structures of the regenerated fibers were investigated using wide-angle X-ray diffraction (WAXD). To date, four crystal polymorphs of α-1,3glucan from polymorph I to IV have been reported. This study revealed that the regenerated α-1,3-glucan fibers had two different polymorphs, polymorph II (hydrated form) and polymorph III (anhydrous form), depending on post-treatment methods of stretching and annealing procedures. Furthermore, the obtained distinctive 2D-WAXD patterns suggested that polymorph III is identical to polymorph IV.

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Section: Introductionmentioning

confidence: 99%

High Tensile Strength Regenerated α-1,3-Glucan Fiber and Crystal Transition

et al. 2021

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Synthesis of paramylon ester-graft-PLA copolymers and its two-step enzymatic degradation by proteinase K and β-1,3-glucanase

Seok

Enomoto-Rogers

Iwata

2022

Polymer Degradation and Stability

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Identification of Genes Involved in the Synthesis of the Fungal Cell Wall Component Nigeran and Regulation of Its Polymerization in Aspergillus luchuensis

Uechi

Yaguchi

Tokashiki

et al. 2021

Appl Environ Microbiol

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Certain Aspergillus and Penicillium spp. produce the fungal cell wall component nigeran, an unbranched D-glucan with alternating α-1,3- and α-1,4-glucoside linkages, under nitrogen starvation. The mechanism underlying nigeran biosynthesis and the physiological role of nigeran in fungal survival are not clear. We used RNA-seq to identify genes involved in nigeran synthesis in the filamentous fungus Aspergillus luchuensis when grown under nitrogen-free conditions. agsB, which encodes a putative α-1,3-glucan synthase, and two adjacent genes ( agtC and gnsA ) were upregulated under conditions of nitrogen starvation. Disruption of agsB in A. luchuensis (Δ agsB ) resulted in the complete loss of nigeran synthesis. Furthermore, overexpression of agsB in an Aspergillus oryzae strain that cannot produce nigeran resulted in nigeran synthesis. These results indicated that agsB encodes a nigeran synthase. Therefore, we have renamed the A . luchuensis agsB as nigeran synthase gene ( nisA ). Nigeran synthesis in an agtC mutant (Δ agtC ) increased to 121%; conversely, that in Δ gnsA and Δ agtCgnsA decreased to 64% and 63%, respectively, compared to that in the wild-type strain. Our results revealed that AgtC and GnsA play an important role in regulating not only the quantity of nigeran but also its polymerization. Collectively, our results demonstrated that nisA ( agsB ) is essential for nigeran synthesis in A . luchuensis, whereas agtC and gnsA contribute to the regulation of nigeran synthesis and its polymerization. This research provides insights into fungal cell wall biosynthesis, specifically the molecular evolution of fungal α-glucan synthase genes and the potential utilization of nigeran as a novel biopolymer. Importance The fungal cell wall is composed mainly of polysaccharides. Under nitrogen-free conditions, some Aspergillus and Penicillium spp. produce significant levels of nigeran, a fungal cell wall polysaccharide composed of alternating α-1,3-/1,4-glucosidic linkages. The mechanisms regulating the biosynthesis and function of nigeran are unknown. Here, we performed RNA sequencing of Aspergillus luchuensis cultured under nitrogen-free or low-nitrogen conditions. A putative α-1,3-glucan synthase gene, whose transcriptional level was upregulated under nitrogen-free conditions, was demonstrated to encode nigeran synthase. Furthermore, two genes encoding an α-glucanotransferase and a hypothetical protein were shown to be involved in controlling nigeran content and molecular weight. This study reveals genes involved in the synthesis of nigeran, a potential biopolymer, and provides a deeper understanding of fungal cell wall biosynthesis.

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Synthesis and characterization of α-1,3-alt-α-1,4-glucan (nigeran) ester derivatives

Cited by 4 publications

References 32 publications

High Tensile Strength Regenerated α-1,3-Glucan Fiber and Crystal Transition

High Tensile Strength Regenerated α-1,3-Glucan Fiber and Crystal Transition

Synthesis of paramylon ester-graft-PLA copolymers and its two-step enzymatic degradation by proteinase K and β-1,3-glucanase

Identification of Genes Involved in the Synthesis of the Fungal Cell Wall Component Nigeran and Regulation of Its Polymerization in Aspergillus luchuensis

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