Synthesis And Cytogenetic Effect Of Magnetic Nanoparticles

Pandey, B. K.; Shahi, A. K.; Srivastava, Nitisha; Kumar, Girjesh; Gopal, Ram

doi:10.5185/amlett.2015.5956

Cited by 14 publications

(3 citation statements)

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“…Regarding the bactericidal activity of CoNPs, the literature suggests that CoNPs can diffuse inside the cell interacting with the cell membrane, producing oxidative stress and finally causing DNA damage [39]. However, currently there is no knowledge of any associated mechanism of CoNPs against parasites.…”

Section: Discussionmentioning

confidence: 99%

Cobalt nanoparticles as novel nanotherapeutics against Acanthamoeba castellanii

et al. 2019

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Background Species of Acanthamoeba are facultative pathogens which can cause sight threatening Acanthamoeba keratitis and a rare but deadly brain infection, granulomatous amoebic encephalitis. Due to conversion of Acanthamoeba trophozoites to resistant cyst stage, most drugs are found to be ineffective at preventing recurrence of infection. This study was designed to test the antiacanthamoebic effects of different cobalt nanoparticles (CoNPs) against trophozoites and cysts, as well as parasite-mediated host cell cytotoxicity. Methods Three different varieties of CoNPs were synthesized by utilizing hydrothermal and ultrasonication methods and were thoroughly characterized by X-ray diffraction and field emission scanning electron microscopy. Amoebicidal, encystation, excystation, and host cell cytopathogenicity assays were conducted to study the antiacanthamoebic effects of CoNPs. Results The results of the antimicrobial evaluation revealed that cobalt phosphate Co 3 (PO 4 ) 2 hexagonal microflakes, and 100 nm large cobalt hydroxide (Co(OH) 2 ) nanoflakes showed potent amoebicidal activity at 100 and 10 µg/ml against Acanthamoeba castellanii as compared to granular cobalt oxide (Co 3 O 4 ) of size 35–40 nm. Furthermore, encystation and excystation assays also showed consistent inhibition at 100 µg/ml. CoNPs also inhibited amoebae-mediated host cell cytotoxicity as determined by lactate dehydrogenase release without causing significant damage to human cells when treated alone. Conclusions To our knowledge, these findings determined, for the first time, the effects of composition, size and morphology of CoNPs against A. castellanii . Co 3 (PO 4 ) 2 hexagonal microflakes showed the most promising antiamoebic effects as compared to Co(OH) 2 nanoflakes and granular Co 3 O 4 . The results reported in the present study hold potential for the development of antiamoebic nanomedicine.

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Section: Discussionmentioning

confidence: 99%

Cobalt nanoparticles as novel nanotherapeutics against Acanthamoeba castellanii

et al. 2019

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“…The identification of chromosomal aberrations was performed based on the literature [ 92 , 93 , 94 , 95 ] and our previous experience [ 96 , 97 ]. The underlying mechanisms of HA-MNPs’ interaction with the cellular nucleus involve the suspension’s uptake at the level of rootlets, diffusion towards the cell cytoplasm, iron cations’ release from the nanoparticle surface, and their penetration into the cell nucleus, where a catalytic effect is supposed to occur.…”

Section: Discussionmentioning

confidence: 99%

A Study of Hyaluronic Acid’s Theoretical Reactivity and of Magnetic Nanoparticles Capped with Hyaluronic Acid

Răcuciu,

Oancea,

Barbu-Tudoran

et al. 2024

Materials

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Hyaluronic acid (HA) has attracted much attention in tumor-targeted drug delivery due to its ability to specifically bind to the CD44 cellular receptor, which is widely expressed on cancer cells. We present HA-capped magnetic nanoparticles (HA-MNPs) obtained via the co-precipitation method, followed by the electrostatic adsorption of HA onto the nanoparticles’ surfaces. A theoretical study carried out with the PM3 method evidenced a dipole moment of 3.34 D and negatively charged atom groups able to participate in interactions with nanoparticle surface cations and surrounding water molecules. The ATR-FTIR spectrum evidenced the hyaluronic acid binding to the surface of the ferrophase, ensuring colloidal stability in the water dispersion. To verify the success of the synthesis and stabilization, HA-MNPs were also characterized using other investigation techniques: TEM, EDS, XRD, DSC, TG, NTA, and VSM. The results showed that the HA-MNPs had a mean physical size of 9.05 nm (TEM investigation), a crystallite dimension of about 8.35 nm (XRD investigation), and a magnetic core diameter of about 8.31 nm (VSM investigation). The HA-MNPs exhibited superparamagnetic behavior, with the magnetization curve showing saturation at a high magnetic field and a very small coercive field, corresponding to the net dominance of single-domain magnetic nanoparticles that were not aggregated with reversible magnetizability. These features satisfy the requirement for magnetic nanoparticles with a small size and good dispersibility for long-term stability. We performed some preliminary tests regarding the nanotoxicity in the environment, and some chromosomal aberrations were found to be induced in corn root meristems, especially in the anaphase and metaphase of mitotic cells. Due to their properties, HA-MNPs also seem to be suitable for use in the biomedical field.

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“…Based on the literature review, the antibacterial mechanism of cobalt nanoparticles is related to oxidative stress. Cobalt nanoparticles induced the production of reactive oxygen species (ROS), which can diffuse into bacteria through the cell membrane, causing DNA damage to bacteria [36]. This might explain the reason that CHP exhibited the most potent anti-acanthamoebic effects against A. castellanii, as CHP contained the highest oxygen percentage content, thereby inducing harsher oxidation stress.…”

Section: Discussionmentioning

confidence: 99%

Effects of Shape and Size of Cobalt Phosphate Nanoparticles against Acanthamoeba castellanii

et al. 2019

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T4 genotype Acanthamoeba are opportunistic pathogens that cause two types of infections, including vision-threatening Acanthamoeba keratitis (AK) and a fatal brain infection known as granulomatous amoebic encephalitis (GAE). Due to the existence of ineffective treatments against Acanthamoeba, it has become a potential threat to all contact lens users and immunocompromised patients. Metal nanoparticles have been proven to have various antimicrobial properties against bacteria, fungi, and parasites. Previously, different types of cobalt nanoparticles showed some promise as anti-acanthamoebic agents. In this study, the objectives were to synthesize and characterize the size, morphology, and crystalline structure of cobalt phosphate nanoparticles, as well as to determine the effects of different sizes of cobalt metal-based nanoparticles against A. castellanii. Cobalt phosphate octahydrate (CHP), Co3(PO4)2•8H2O, was synthesized by ultrasonication using a horn sonicator, then three different sizes of cobalt phosphates Co3(PO4)2 were produced through calcination of Co3(PO4)2•8H2O at 200 °C, 400 °C and 600 °C (CP2, CP4, CP6). These three types of cobalt phosphate nanoparticles were characterized using a field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDX), and X-ray diffraction (XRD) analysis. Next, the synthesized nanoparticles were subjected to biological assays to investigate their amoebicidal, amoebistatic, anti-encystation, and anti-excystation effects against A. castellanii, as well as cell cytotoxicity. The overall results showed that 1.30 ± 0.70 µm of CHP microflakes demonstrated the best anti-acanthemoebic effects at 100 µg/mL, followed by 612.50 ± 165.94 nm large CP6 nanograins. However, amongst the three tested cobalt phosphates, Co3(PO4)2, the smaller nanoparticles had stronger antiamoebic effects against A. castellanii. During cell cytotoxicity analysis, CHP exhibited only 15% cytotoxicity against HeLa cells, whereas CP6 caused 46% (the highest) cell cytotoxicity at the highest concentration, respectively. Moreover, the composition and morphology of nanoparticles is suggested to be important in determining their anti-acathamoebic effects. However, the molecular mechanisms of cobalt phosphate nanoparticles are still unidentified. Nevertheless, the results suggested that cobalt phosphate nanoparticles hold potential for development of nanodrugs against Acanthamoeba.

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Synthesis And Cytogenetic Effect Of Magnetic Nanoparticles

Cited by 14 publications

References 0 publications

Cobalt nanoparticles as novel nanotherapeutics against Acanthamoeba castellanii

Cobalt nanoparticles as novel nanotherapeutics against Acanthamoeba castellanii

A Study of Hyaluronic Acid’s Theoretical Reactivity and of Magnetic Nanoparticles Capped with Hyaluronic Acid

Effects of Shape and Size of Cobalt Phosphate Nanoparticles against Acanthamoeba castellanii

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