Trialkylammonium 4-(N)-1,1Ј,2-trisnor-squalenoylgemcitabine monophosphate (SQdFdC-MP) salts were prepared from 1,1Ј,2-trisnor-squalenoylgemcitabine by phosphoramiditetype chemistry. This amphiphilic molecule self-assembled into nanoassemblies of about 100 nm in size in aqueous solutions. Cryo-TEM and small-angle X-ray scattering (SAXS) investigations revealed that SQdFdC-MP molecules selforganized into unilamellar liposomes with a membrane