Synthesis and effect of modification on methacylate - acrylate microspheres for Trametes versicolor laccase enzyme immobilization

Mazlan, Siti Zulaikha; Hanifah, Sharina Abu

doi:10.1063/1.4895206

Cited by 4 publications

(2 citation statements)

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“…The prepared polymer microspheres were used for laccase immobilization via covalent linkage. Among the great variety of enzymes, laccase has gained much attention for being capable of catalyzing a wide range of popular reactions such as delignification, waste detoxification and decontamination, decolourization of dyes, degradation of polycyclic aromatic hydrocarbons, and bioremediation and as bioreceptor in biosensors application [17,18]. Thus, laccase was chosen as an enzyme to understand the interaction of epoxy-immobilized laccase conjugated methacrylate-acrylate copolymer microspheres.…”

Section: Introductionmentioning

confidence: 99%

Effects of Temperature and pH on Immobilized Laccase Activity in Conjugated Methacrylate-Acrylate Microspheres

Mazlan

Hanifah

2017

International Journal of Polymer Science

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Immobilization of laccase on the functionalized methacrylate-acrylate copolymer microspheres was studied. Poly(glycidyl methacrylate-co-n-butyl acrylate) microspheres consisting of epoxy groups were synthesized using facile emulsion photocuring technique. The epoxy groups in poly(GMA-co-nBA) microspheres were then converted to amino groups. Laccase immobilization is based on covalent binding via amino groups on the enzyme surface and aldehyde group on the microspheres. The FTIR spectra showed peak at 1646 cm −1 assigned to the conformation of the polymerization that referred to GMA and nBA monomers, respectively. After modification of the polymer, intensity of FTIR peaks assigned to the epoxy ring at 844 cm −1 and 904 cm −1 was decreased. The results obtained from FTIR exhibit a good agreement with the epoxy content method. The activity of laccaseimmobilized microspheres increased upon increasing the epoxy content. Furthermore, poly(GMA-co-nBA) microspheres revealed uniform size below 2 m that contributes to large surface area of the microspheres to be used as a matrix, thus increasing the enzyme capacity and enzymatic reaction. Immobilized enzyme also shifted to higher pH and temperature compared to free enzyme.

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Section: Introductionmentioning

confidence: 99%

Effects of Temperature and pH on Immobilized Laccase Activity in Conjugated Methacrylate-Acrylate Microspheres

Mazlan

Hanifah

2017

International Journal of Polymer Science

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“…In this work, laccase enzyme was immobilized on poly(glycidyl methacrylate- co -n-butyl acrylate) (poly(GMA- co -nBA)) microspheres, which we have reported recently [ 18 ]. Poly (n-butyl acrylate) has a hydrophobic property and thus the microspheres are hydrophobic where the laccase immobilization will be confined to the surface of the spheres; this allows the enzymatic reaction with tartrazine to occur at the surface and diffusion limitation within the polymer matrix is eliminated [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

A New Laccase Based Biosensor for Tartrazine

Mazlan

Lee

Hanifah

2017

Sensors

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Laccase enzyme, a commonly used enzyme for the construction of biosensors for phenolic compounds was used for the first time to develop a new biosensor for the determination of the azo-dye tartrazine. The electrochemical biosensor was based on the immobilization of laccase on functionalized methacrylate-acrylate microspheres. The biosensor membrane is a composite of the laccase conjugated microspheres and gold nanoparticles (AuNPs) coated on a carbon-paste screen-printed electrode. The reaction involving tartrazine can be catalyzed by laccase enzyme, where the current change was measured by differential pulse voltammetry (DPV) at 1.1 V. The anodic peak current was linear within the tartrazine concentration range of 0.2 to 14 μM (R2 = 0.979) and the detection limit was 0.04 μM. Common food ingredients or additives such as glucose, sucrose, ascorbic acid, phenol and sunset yellow did not interfere with the biosensor response. Furthermore, the biosensor response was stable up to 30 days of storage period at 4 °C. Foods and beverage were used as real samples for the biosensor validation. The biosensor response to tartrazine showed no significant difference with a standard HPLC method for tartrazine analysis.

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Recent Progress in Intensifying Synthesis of Acrylic Microspheres for Catalysis

Gaddale

Kale

Srinivasan

et al. 2023

Adv Materials Inter

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Over the past decades, there has been an escalating rise in the need for chemicals and catalytic materials to keep up with global demands. Addressing those issues by conventional methods often becomes inefficient, with myriad operational risks. Process intensification methods through procedural and equipment‐based modifications have been considered greener, have higher heat and mass transfer rates, and operate with lower costs. In this review, research using ultrasonic reactors and microreactors, along with developments through an integrated external energy source, for synthesizing acrylic microspheres is covered extensively. Acrylic microspheres have garnered much interest for their biocompatibility, affinity toward functionalization, and wide range of applications. Core–shell, composite, functional‐group modified, and porous acrylic microspheres are used for enzyme immobilization and as catalyst carriers. The use of acrylic support has provided huge improvements in catalytic activity, reusability, recyclability, and overall stability. Finally, various other process intensification methods and alternate support materials are covered to help enhance future developments in the field of catalysis.

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Synthesis and effect of modification on methacylate - acrylate microspheres for Trametes versicolor laccase enzyme immobilization

Cited by 4 publications

References 0 publications

Effects of Temperature and pH on Immobilized Laccase Activity in Conjugated Methacrylate-Acrylate Microspheres

Effects of Temperature and pH on Immobilized Laccase Activity in Conjugated Methacrylate-Acrylate Microspheres

A New Laccase Based Biosensor for Tartrazine

Recent Progress in Intensifying Synthesis of Acrylic Microspheres for Catalysis

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