Synthesis and evaluation of monoclonal antibody against Plasmodium falciparum merozoite surface antigen 2

Khosravi, Afra; Asadollahy, Eghbaleh; Ghafourian, Sobhan; Sadeghifard, Nourkhoda; Mohebi, Reza

doi:10.1016/s1995-7645(13)60141-8

Cited by 3 publications

(1 citation statement)

References 15 publications

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“…Plasmodium berghei antigen was prepared from parasitized Red Blood Cells (pRBCs) obtained from BALB/c mice (12). Four ml of heparinized blood was washed 3 times with PBS at 2000 rpm for 6 minutes.…”

Section: Preparation Of Plasmodium Berghei Antigenmentioning

confidence: 99%

Concomitant infection withLeishmania donovaniandPlasmodium berghei altersclinical and immune responses in BALB/c mice

Mutiso

Macharia

et al. 2021

Preprint

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Malaria and visceral leishmaniasis coexist in the same geographical regions. However, dual co-infection with parasites causing these diseases and their impact on public health is poorly documented. Interactions between these parasites may play a role in disease outcome. The present study set out to evaluate the clinical and immunological parameters following Leishmania donovani and Plasmodium berghei co-infection in BALB/c mice. Mice were divided into four groups; L. donovani- only, L. donovani- P. berghei , P. berghei- only and naïve. Body weight, parasite burden, total IgG, IFN-γ and IL-4 responses were determined. To determine the survival rate, four mice were used from each group. Tissues for histological analysis were taken from spleen, liver and brain. Results indicated significant differences in body weight (P<0.0001), L. donovani parasite load (P< 0.0001 ), L. donovani IgG (P< 0.0001), P. berghei parasitemia (P= 0.0222), P. berghei IgG (P= 0.002), IFN-γ (P<0.0001) and IL-4 (P<0.0001) in dual-infected mice. There was no correlation between L. donovani parasite load and IgG responses in single or dual infections, while there was a positive relationship of P. berghei parasitemia and IgG responses in the dual infection group only. Plasmodium berghei had the highest mortality rate compared to L. donovani - only and L. donovani- P. berghei infected mice groups. Histological analyses showed enlarged red and white pulps and pathological changes in the spleen, liver and brain tissues which were less pronounced in co-infected group. We conclude that L. donovani and P. berghei co-infection reduces disease severity and these changes seem to correlate with variation in serum IgG and cytokines (IFN-γ and IL-4). Therefore, the study recommends the importance of inclusion of early screening of malaria in Visceral Leishmaniasis patients in regions where malaria is co- endemic.

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Section: Preparation Of Plasmodium Berghei Antigenmentioning

confidence: 99%

Concomitant infection withLeishmania donovaniandPlasmodium berghei altersclinical and immune responses in BALB/c mice

Mutiso

Macharia

et al. 2021

Preprint

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A Dual, Systematic Approach to Malaria Diagnostic Biomarker Discovery

Yerlikaya

Owusu

Frimpong

et al. 2021

Clinical Infectious Diseases

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Background The emergence and spread of P. falciparum parasites lacking HRP2/3 proteins and the resulting decreased utility of HRP2-based malaria rapid diagnostic tests (RDTs) prompted the World Health Organization (WHO) and other global health stakeholders to prioritize the discovery of novel diagnostic biomarkers for malaria. Methods To address this pressing need, we adopted a dual, systematic approach by conducting a systematic review of literature for publications on diagnostic biomarkers for uncomplicated malaria and a systematic in silico analysis of P. falciparum proteomics data for Plasmodium proteins with favorable diagnostic features. Results Our complementary analyses led us to two novel malaria diagnostic biomarkers compatible for use in an RDT format: glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and dihydrofolate reductase-thymidylate synthase (DHFR-TS). Conclusion Overall, our results pave the way for the development of next generation malaria RDTs based on new antigens by identifying two lead candidates with favorable diagnostic features and partially de-risked product development prospects.

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Concomitant Infection with <i>Leishmania donovani</i> and <i>Plasmodium berghei </i>Causes Pro-inflammatory Polarization Resulting in Malaria Exacerbation in BALB/c Mice

Ayako¹,

Mutiso²,

Macharia³

et al. 2021

AJIDM

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Synthesis and evaluation of monoclonal antibody against Plasmodium falciparum merozoite surface antigen 2

Abstract: Bringing together all the results of this study it has been confirmed that some clones have recognized both schizont extract and different domains of the MSP-2 recombinant protein and therefore confirming the quality of the MSP-2 domains.

Cited by 3 publications

References 15 publications

Concomitant infection withLeishmania donovaniandPlasmodium berghei altersclinical and immune responses in BALB/c mice

Concomitant infection withLeishmania donovaniandPlasmodium berghei altersclinical and immune responses in BALB/c mice

A Dual, Systematic Approach to Malaria Diagnostic Biomarker Discovery

Concomitant Infection with <i>Leishmania donovani</i> and <i>Plasmodium berghei </i>Causes Pro-inflammatory Polarization Resulting in Malaria Exacerbation in BALB/c Mice

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