Synthesis and fundamental studies of a photoresponsive oligonucleotide-upconverting nanoparticle covalent conjugate

Abstract

A greener route to a photocleavable phosphoramidite was used to synthesize an alkyne-containing oligonucleotide and conjugated to an azide-modified upconverting nanoparticle. The conjugation, energy transfer, and photocleavage were evaluated.

Silica-coated LiYF4:Yb3+, Tm3+ upconverting nanoparticles are non-toxic and activate minor stress responses in mammalian cells

“…This agrees with our previously reported values and the intrinsic negative charge of silica. 60,61 Azidefunctionalized silica produces a more negative surface charge due to the negative charge on the terminal nitrogen atoms in the azide groups. 62 In cell culture medium (DMEM), the surface charges of both NPs were reduced to −4.1 ± 0.1 mV for Si-UCNPs and −9.0 ± 0.5 mV for AzSi-UCNPs.…”