Synthesis and<i>in vitro</i>evaluation of thermosensitive hydrogel scaffolds based on (PNIPAAm-PCL-PEG-PCL-PNIPAAm)/Gelatin and (PCL-PEG-PCL)/Gelatin for use in cartilage tissue engineering

Saghebasl, Solmaz; Davaran, Soodabeh; Rahbarghazi‬, Reza; Montaseri, Azadeh; Salehi, Roya; Ramazani, Ali

doi:10.1080/09205063.2018.1447627

Cited by 64 publications

(42 citation statements)

References 38 publications

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“…Size-exclusion chromatography (SEC) is normally employed to determine the molecular weight and molecular weight distribution of PNIPAAm and derivatives [64,65]. Static light scattering (SLS) enables to measure the average molecular weight of macromolecules in PNIPAAm solution [66], but this technique requires the system to be sensitive and exceptionally stable.…”

Section: Molecular Weightmentioning

confidence: 99%

“…The optical transmittances of the solutions are monitored at 500 nm wavelength via UV-Vis spectrometer with incremental temperature interval. The inflection point of turbidity plots is regarded as the LCST of the synthetic polymers [65].…”

Section: Volume Phase Transition Temperature/lower Critical Solution mentioning

confidence: 99%

“…Many techniques used for the evaluation of thermoresponsive substrates are also capable of determining the chemical structures of thermoresponsive scaffolds, which are frequently characterized using ATR-FTIR, NMR, XPS and SEM/EDS systems [65,[102][103][104][105]. Grafting yield of PNIPAAm mediated scaffolds can be assessed by analyzing the ratio of peak intensities of characteristic and reference peaks from ATR-FTIR spectra.…”

Section: Chemical Featuresmentioning

confidence: 99%

“…Scaffold microstructure (surface and cross-section) is commonly evaluated by SEM, AFM and light microscope like thermoresponsive macrogels [102][103][104][106][107][108]. Pore diameter and distribution or fiber size and alignment are often analyzed by randomly picking at least dozens of various segments by means of image J software [65,109]. Individual scaffold dimensions can be measured with digital calipers [108,110].…”

Section: Morphology and Structurementioning

confidence: 99%

“…The criteria for excellent displacement liquid is that it can easily penetrate into the scaffold pores and does not cause a change in volume, and the solvent will not dissolve the prepared scaffolds. The density quantities give useful information concerning aperture, dispersity, penetrability and structural deficiency of scaffolds [38,65].…”

Section: Morphology and Structurementioning

confidence: 99%

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Preparation and Characterization of Thermoresponsive Poly(N-Isopropylacrylamide) for Cell Culture Applications

et al. 2020

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Poly(N-isopropylacrylamide) (PNIPAAm) is a typical thermoresponsive polymer used widely and studied deeply in smart materials, which is attractive and valuable owing to its reversible and remote "on-off" behavior adjusted by temperature variation. PNIPAAm usually exhibits opposite solubility or wettability across lower critical solution temperature (LCST), and it is readily functionalized making it available in extensive applications. Cell culture is one of the most prospective and representative applications. Active attachment and spontaneous detachment of targeted cells are easily tunable by surface wettability changes and volume phase transitions of PNIPAAm modified substrates with respect to ambient temperature. The thermoresponsive culture platforms and matching thermal-liftoff method can effectively substitute for the traditional cell harvesting ways like enzymatic hydrolysis and mechanical scraping, and will improve the stable and high quality of recovered cells. Therefore, the establishment and detection on PNIPAAm based culture systems are of particular importance. This review covers the important developments and recommendations for future work of the preparation and characterization of temperature-responsive substrates based on PNIPAAm and analogues for cell culture applications.

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Section: Molecular Weightmentioning

confidence: 99%

Section: Volume Phase Transition Temperature/lower Critical Solution mentioning

confidence: 99%

Section: Chemical Featuresmentioning

confidence: 99%

Section: Morphology and Structurementioning

confidence: 99%

Section: Morphology and Structurementioning

confidence: 99%

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Preparation and Characterization of Thermoresponsive Poly(N-Isopropylacrylamide) for Cell Culture Applications

et al. 2020

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Regulation and Reconstruction of Cell Phenotype Gradients Along the Tendon‐Bone Interface

Dang

Qin

Wan

et al. 2022

Adv Funct Materials

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Tendon-bone interface is prevalent in the human body. It is divided into four zones: tendon (soft tissue), unmineralized fibrocartilage, mineralized fibrocartilage, and bone (hard tissue). Tendon-bone interface is characterized by a cell phenotype gradient that appears in the different zones. The cell phenotype gradients at the tendon-bone interface are orchestrated by specific intracellular molecular mechanisms, extracellular factors, immune signals, and neurovascular factors. These features have inspired scientists to design systems that mimic natural cell phenotype gradients. These biomimetic systems include the construction of cell sheets, regulation of cellular microenvironments, and the design of gradient functional scaffolds. Exploration of methods to mimic cell phenotype gradients is instructional for future clinical applications in reconstituting the tendon-bone interface. The present review elucidates the gradient composition of the tendon-bone interface. The associated regulatory mechanisms and applications are discussed, with the anticipation of creating a mise en scène for future research in interface tissue engineering.

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Progress of Microfluidic Hydrogel‐Based Scaffolds and Organ‐on‐Chips for the Cartilage Tissue Engineering

et al. 2023

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Cartilage degeneration is among the fundamental reasons behind disability and pain across the globe. Numerous approaches have been employed to treat cartilage diseases. Nevertheless, none have shown acceptable outcomes in the long run. In this regard, the convergence of tissue engineering and microfabrication principles can allow developing more advanced microfluidic technologies, thus offering attractive alternatives to current treatments and traditional constructs used in tissue engineering applications. Herein, the current developments involving microfluidic hydrogel‐based scaffolds, promising structures for cartilage regeneration, ranging from hydrogels with microfluidic channels to hydrogels prepared by the microfluidic devices, that enable therapeutic delivery of cells, drugs, and growth factors, as well as cartilage‐related organ‐on‐chips are reviewed. Thereafter, cartilage anatomy and types of damages, and present treatment options are briefly overviewed. Various hydrogels are introduced, and the advantages of microfluidic hydrogel‐based scaffolds over traditional hydrogels are thoroughly discussed. Furthermore, available technologies for fabricating microfluidic hydrogel‐based scaffolds and microfluidic chips are presented. The preclinical and clinical applications of microfluidic hydrogel‐based scaffolds in cartilage regeneration and the development of cartilage‐related microfluidic chips over time are further explained. The current developments, recent key challenges, and attractive prospects that should be considered so as to develop microfluidic systems in cartilage repair are highlighted.

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Synthesis andin vitroevaluation of thermosensitive hydrogel scaffolds based on (PNIPAAm-PCL-PEG-PCL-PNIPAAm)/Gelatin and (PCL-PEG-PCL)/Gelatin for use in cartilage tissue engineering

Cited by 64 publications

References 38 publications

Preparation and Characterization of Thermoresponsive Poly(N-Isopropylacrylamide) for Cell Culture Applications

Preparation and Characterization of Thermoresponsive Poly(N-Isopropylacrylamide) for Cell Culture Applications

Regulation and Reconstruction of Cell Phenotype Gradients Along the Tendon‐Bone Interface

Progress of Microfluidic Hydrogel‐Based Scaffolds and Organ‐on‐Chips for the Cartilage Tissue Engineering

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