SYNTHESIS AND IMMUNOLOGICAL EFFECT OF THYMIC HUMORAL FACTOR-γ 2 ANALOGUES

Abstract

Nine analogues of thymic humoral factor (THF)-gamma 2 were prepared by the solid-phase method and their in vitro restoring effect on the impaired blastogenic response of phytohemagglutinin(PHA)-stimulated T-lymphocytes of uremic patients with infectious diseases were examined. The results were as follows: [Arg6]-THF-gamma 2 exhibited higher restoring activity than that of our synthetic THF-gamma 2. [Sar4]-, [Val1]-, [Arg3]-, [Gly5]-, and [Asn3]-THF-gamma 2 were also active, but less potent than that of our synthetic THF-gamma 2. Three other peptides, [beta Ala4]-, [Arg2]-, and [Gln2]-THF-gamma 2, did not show any restoring activity on the impaired blastogenic response of uremic patients with infectious disease.

Degradation of thymic humoral factor γ2 in human, rat and mouse blood: An experimental and theoretical study

“…Studies with synthetic analogues of THF have shown the Phe residue at position 7 to be important to its effects on the impaired blastogenic response of phytohemagglutinin(PHA)-stimulated T-lymphocytes from uremic patients with infectious diseases [25,26]. The degradation patterns in each of the species indicate that the facility in which this is removed by cleavage of the Lys-Phe bond, to yield peptides 1-6 and 2-6, may be an important factor in limiting the effectiveness of THF-γ2 in vivo.…”