Synthesis and regulation of extracellular keratinase in three fungi isolated from the grounds of a gelatin factory, Jabalpur, India

Malviya, H. K.; Rajak, R. C.; Hasija, S. K.

doi:10.1007/bf00578494

Cited by 32 publications

(15 citation statements)

References 14 publications

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“…This result is in accordance with the previous findings of Son et al (2008) and Malviya et al (1992) for B.…”

Section: Cheng Et Al (1995) Reported Complete Inhibition Of the Kerasupporting

confidence: 94%

Biosynthesis and Control of Keratinase in Recalcitrant Feather-Degrading Bacillus megaterium F7-1

Jeong¹,

Lee²,

Jeon³

et al. 2010

Journal of Environmental Science International

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This study was performed to investigate the nutritional conditions controlling keratinase activity in Bacillus megaterium F7-1. B. megaterium F7-1 produced keratinase using chicken feather as a sole source of carbon, nitrogen and sulfur. Addition of the feather medium with glucose enhanced keratinase production (68.9 U/ml), compared to control without glucose (63.2 U/ml). The synthesis of keratinase was repressed by addition of NH4Cl in B. megaterium F7-1. The highest keratinase production (70.9 U/ml) was obtained with the feather medium containing glucose and MgSO4·7H2O. Keratinase was produced in the absence of feather (4.9 U/ml), indicating its constitutive synthesis. Feather degradation resulted in free SH group formation. B. megaterium F7-1 effectively degraded chicken feather meal (86%), whereas duck feather, human nail, human hair and sheep wool displayed relatively low degradation rates (8-34%).

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“…This result is in accordance with the previous findings of Son et al (2008) and Malviya et al (1992) for B.…”

Section: Cheng Et Al (1995) Reported Complete Inhibition Of the Kerasupporting

confidence: 94%

Biosynthesis and Control of Keratinase in Recalcitrant Feather-Degrading Bacillus megaterium F7-1

Jeong¹,

Lee²,

Jeon³

et al. 2010

Journal of Environmental Science International

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“…Concerning the use of nitrogen sources diVerent from keratin, an inductive eVect of soybean meal has been reported to occur [14]. However, most reports describe a partial or complete repressive eVect of the supplementation of cultures with small nitrogen molecules [8,19,36].…”

Section: Introductionmentioning

confidence: 95%

“…Among fungi, keratinolytic activity from dermatophytes has received more attention due to medical and veterinary implications [28,37]. Although some nondermatophytic fungi have already been described [1,2,10,14,15,19,21,33], for biotechnological purposes, bacterial keratinases have been received more attention, particularly those produced by Bacillus [5,13,20,36]. Keratinolytic enzymes Wnd use in environment friendly biotechnological processes that use keratin-containing wastes from poultry industry [16,29].…”

Section: Introductionmentioning

confidence: 99%

Influence of the carbon and nitrogen sources on keratinase production by Myrothecium verrucaria in submerged and solid state cultures

Gioppo¹,

Moreira-Gasparin²,

Costa³

et al. 2009

J Ind Microbiol Biotechnol

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Myrothecium verrucaria is a nondermatophytic filamentous fungus able to grow and to produce keratinase in submerged (93.0 +/- 19 U/ml) and solid state (98.8 +/- 7.9 U/ml) cultures in which poultry feather powder (PFP) is the only substrate. The purpose of the present work was to verify how different carbon and nitrogen sources can influence the production of keratinase by this fungus. Addition of carbohydrates, such as glucose and sucrose, caused only slight improvements in keratinase production, but the addition of starch caused a significant improvement (135.0 +/- 25 U/ml). The highest levels of keratinase activity, however, were obtained by supplementing the PFP cultures with cassava bagasse, 168.0 +/- 28 U/ml and 189.0 +/- 26 U/ml in submerged and solid state cultures, respectively. Contrarily, the supplementation of PFP medium with organic or inorganic nitrogen sources, such as casein, soy bean protein, gelatin, ammonium nitrate and alanine, decreased the production of keratinase in both types of cultures (around 20 U/ml), showing that the production of keratinase by M. verrucaria is repressed by nitrogen sources. The results obtained in this work suggest that the association of the two residues PFP plus cassava bagasse could be an excellent option as a cheap culture medium for the production of keratinase in submerged and solid state cultures.

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“…This result is congruent with that reported by Cai and Zheng (2009) for the production of keratinase by Bacillus subtilis KD-N2. Similarly, Malviya et al (1992) and El-Naghy et al (1998) reported maximum keratinase production from Chrysosporium queenslandicum and C. georgiae, respectively, in the presence of keratin, which was further stimulated by the addition of glucose. On the contrary, production of keratinase by Streptomyces sp.…”

Section: Influence Of Additional Carbon Source On Keratinase Productionmentioning

confidence: 80%

Optimization of Physico-Chemical Parameters for Hyper Keratinase Production from a Newly Isolated Aspergillus sp. DHE7 using Chicken Feather as Substrate- Management of Biowaste

2017

J App Pharm Sci

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Keratinases have recently gained biotechnological impetus due to their ability in degrading insoluble keratinous solid wastes into simplified forms, which used in the preparation of nitrogenous fertilizers, biodegradable films, glues and foils. Keratinases are widespread in nature and mainly produced by diverse microorganisms including bacteria, fungi and actinomycetes. Therefore, the aim of the present work was to isolate and identify a new potent fungal strain possessing extracellular keratinase production capacity using keratinous solid wastes as the sole carbon and nitrogen sources. Fifteen keratinolytic fungi, isolated from a poultry farm soil using white chicken feathers as substrate, were screened qualitatively and quantitatively for their ability to produce extracellular keratinase. Medium optimization for enzyme production was also studied under submerged fermentation conditions. The results revealed that a new fungus identified as Aspergillus sp. DHE7 was reported as the most potent fungal strain for extracellular keratinase production under submerged shaking conditions (120 rpm). The highest yield of enzyme of 199+4.2 U/mL was observed after four days of incubation at pH 6.0 and temperature 30 °C using 2 % of chicken feathers as substrate. Medium supplementation with 0.5 % sucrose as an additional source of carbon increased keratinase yield to 226+5.4 U/mL. While using additional nitrogen sources could not promote a favorable effect on keratinase productivity as compared with the basal medium. Different keratinous wastes were used as substrate in the basal medium and better keratinase production was obtained with goat hair medium (452+12.3 U/mL) followed by turkey feather (435+9.2 U/mL) and sheep wool (322+13.4 U/mL) media. The present data suggest Aspergillus sp. DHE7 as a potential keratinolytic strain for the bioconversion of keratinous wastes, through a non-polluting process.

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Synthesis and regulation of extracellular keratinase in three fungi isolated from the grounds of a gelatin factory, Jabalpur, India

Cited by 32 publications

References 14 publications

Biosynthesis and Control of Keratinase in Recalcitrant Feather-Degrading Bacillus megaterium F7-1

Biosynthesis and Control of Keratinase in Recalcitrant Feather-Degrading Bacillus megaterium F7-1

Influence of the carbon and nitrogen sources on keratinase production by Myrothecium verrucaria in submerged and solid state cultures

Optimization of Physico-Chemical Parameters for Hyper Keratinase Production from a Newly Isolated Aspergillus sp. DHE7 using Chicken Feather as Substrate- Management of Biowaste

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